Effect of crude oil-induced water repellency on transport of Escherichia coli and bromide through repacked and physically-weathered soil columns.

Knowledge of the transport and fate of pathogenic Escherichia coli, especially in the areas contaminated with crude oil, is required to assess contamination of shallow groundwater resources. The present study aims to investigate the effect of crude oil-mediated water repellency on the movement of nalidixic acid-resistant Escherichia coli strain (E. coli NAR) and bromide (Br) as an inert tracer in two soil types. The soils were contaminated at three levels of 0, 0.5 and 1% w/w of total petroleum hydrocarbons (TPHs) using crude oil. Steady-state saturated flow in the soil columns was controlled using a tension infiltrometer. Leaching experiments were conducted through the columns of repacked (un-weathered) and physically-weathered clay loam (CL) and sandy loam (SL) soils. The columns leachate was sampled at specific times for 4 pore volumes. The shape of breakthrough curves for the E. coli NAR and Br depended on soil texture and structure and the TPHs level. Preferential flow in the crude oil-mediated water-repellent soils facilitated the transport of contaminants especially E. coli NAR. Filtration coefficient and relative adsorption index of bacteria were greatest in the repacked CL soils and were lowest in the weathered SL soils. Discontinuity of soil pores and lower flow velocity resulted in greater filtration of E. coli NAR in the repacked CL soil than other treatments. Physical weathering induced the formation of aggregates which reduced soil particle surfaces available for retention of water-repellent oil and contaminants. Movement of both bacteria and Br tracer in the weathered SL soil with 1% TPHs was higher than other treatments. This finding was attributed to low specific surface area, continuity of the pores and water repellency-mediated preferential pathways in the weathered SL soil columns. Our findings implied that shallow groundwater resources could be very sensitive to microbial contamination particularly in the oil-mediated water-repellent soils.

Significance of physical weathering of two-texturally different soils for the saturated transport of Escherichia coli and bromide.

This study was carried out to investigate the transport of Escherichia coli NAR and bromide (Br) through repacked (R) and weathered (W) soil columns. A suspension containing E. coli NAR and Br were leached and the effluent from the weathered soil columns had greater contaminant concentrations than that from the repacked soil columns. The time to the concentration peak of (C(max)) E. coli NAR and Br increased in the order CL-W < SL-W < SL-R < CL-R. The breakthrough sequence suggests the formation of a heterogeneous soil pore network induced by weathering and the importance of accelerated flow in the weathered columns. The dual-permeability model in HYDRUS-1D software was used to simulate the E. coli NAR and Br transport parameters by inverse modeling. Parameters of the attachment-detachment model were calculated using the dual-permeability model parameters fitted to the BTCs of E. coli NAR. A greater attachment coefficient associated with soil repacking and the finer textured clayey soil demonstrated the importance of adsorbent site and smaller pore spacing in these treatments. Smaller attachment and adsorption isotherm coefficients in weathered soil columns suggest the need for further research to validate this as a predictive model for the risks for vadose zone contaminant transport.