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1.
Braz. J. Pharm. Sci. (Online) ; 54(4): e17811, 2018. tab, graf, ilus
Article in English | LILACS | ID: biblio-1001564

ABSTRACT

Seaweeds constitutes an abundant marine reserve that can be harnessed as source of new pharmaceutical agents. Sargassum binderi Sonder ex J. Agardh is a brown seaweed that is predominantly available from December to March in the Red Sea, Jazan, Kingdom of Saudi Arabia (KSA). In this study, three extracts were isolated using three different techniques, and were subjected to antibacterial assay. The petroleum ether extract of Sargassum binderi was more effective against selected human pathogenic bacteria than the other extracts. Therefore, further studies were focused on developing oleic acid vesicles entrapped with the petroleum ether extract of Sargassum binderi, with the aim of enhancing its penetration property. Oleic acid vesicles were prepared by entrapping petroleum ether extract of Sargassum binderi using film hydration technique. The formulated vesicles were in nanoscale, and so were termed phyto-nanovesicles (PNVs). The spectrum of antibacterial activity of PNVs showed that it is a promising formulation against S. aureus, S. pyogenes, B. subtilis, E. coli, K. pneumoniae and P. aeruginosa. The microbial sensitivities to the PNVs was in the order E.coli > B. subtilis > S. aureus > S. pyogenes > K. pneumoniae > P. aeruginosa. Thus, the PNV formulation possesses promising and effective antimicrobial potential against human pathogenic bacteria


Subject(s)
Indian Ocean/ethnology , Sargassum/metabolism , Seaweed/classification , Anti-Bacterial Agents
2.
Neurochem Res ; 39(2): 344-52, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24379109

ABSTRACT

Inflammatory process has a fundamental role in the pathogenesis of Alzheimer's disease and insoluble amyloid beta deposits and neurofibrillary tangles provide the obvious stimuli for inflammation. The present study demonstrate the effect of pretreatment of 1,8-cineole (Cin) on inflammation induced by Aß(25-35) in differentiated PC12 cells. The cells were treated with Cin at different doses for 24 h and then replaced by media containing Aß(25-35) for another 24 h. The cell viability was decreased in Aß(25-35) treated cells which was significantly restored by Cin pretreatment. Cin successfully reduced the mitochondrial membrane potential, ROS and NO levels in Aß(25-35) treated cells. Cin also lowered the levels of proinflammatory cytokines TNF-α, IL-1ß and IL-6 in Aß(25-35) treated cells. Moreover, Cin also succeeded in lowering the expression of NOS-2, COX-2 and NF-κB. This study suggests the protective effects of Cin on inflammation and provides additional evidence for its potential beneficial use in therapy as an anti-inflammatory agent in neurodegenerative disease.


Subject(s)
Alzheimer Disease/pathology , Cyclohexanols/pharmacology , Inflammation/prevention & control , Monoterpenes/pharmacology , Amyloid beta-Peptides/physiology , Animals , Cytokines/metabolism , Eucalyptol , Membrane Potential, Mitochondrial/drug effects , Oxidative Stress , PC12 Cells , Peptide Fragments/physiology , Rats , Reactive Oxygen Species/metabolism
3.
Neuroscience ; 230: 157-71, 2013 Jan 29.
Article in English | MEDLINE | ID: mdl-23103795

ABSTRACT

Oxidative stress and inflammation play an integral role in the pathogenesis of cerebral ischemia that leads to a cascade of events culminating in the death of neurons and their supporting structures. The signaling pathways that link these events are not fully understood. Recent studies have demonstrated a close link between the nuclear factor-κB (NF-κB) signaling pathway and cerebral ischemia/reperfusion (I/R)-induced inflammation. Flavonoids have been suggested to exert human health benefits by anti-oxidant and anti-inflammatory mechanisms. In this study we undertook a pharmacological approach to investigate the ability of naringenin, a potent flavonoid, to prevent oxidative stress and NF-κB-mediated inflammatory brain damage in the rat model of focal cerebral I/R injury. To test this hypothesis, male Wistar rats were pretreated with naringenin once daily for 21 days and then subjected to 1h of middle cerebral artery occlusion followed by 23 h of reperfusion. Naringenin treatment successfully upregulates the antioxidant status, decreases the infarct size and lowers the levels of myeloperoxidase, nitric oxide and cytokines, besides functional recovery returned close to the baseline. Moreover, immunohistochemical and Western blot analyses clearly demonstrated that naringenin treatment limits glial activation and downregulates the NF-κB expression level and their target genes. These results show, prophylactic treatment with naringenin improved functional outcomes and abrogated the ischemic brain injury by suppressing NF-κB-mediated neuroinflammation. The present study suggests that naringenin may be used as a potential neuroprotectant in patients at high risk of ischemic stroke.


Subject(s)
Flavanones/therapeutic use , Infarction, Middle Cerebral Artery/drug therapy , NF-kappa B/metabolism , Neuroprotective Agents/therapeutic use , Signal Transduction/drug effects , Animals , Brain Infarction/etiology , Brain Infarction/prevention & control , Cytokines/metabolism , Disease Models, Animal , Glial Fibrillary Acidic Protein/metabolism , Glutathione/metabolism , Hand Strength/physiology , Infarction, Middle Cerebral Artery/complications , Lipid Peroxidation/drug effects , Male , Motor Activity/drug effects , Nervous System Diseases/etiology , Nervous System Diseases/prevention & control , Nitric Oxide/metabolism , Peroxidase/metabolism , Peroxisome Proliferator-Activated Receptors/metabolism , Psychomotor Performance/drug effects , Rats , Rats, Wistar , Reperfusion , Thiobarbituric Acid Reactive Substances/metabolism
4.
Neuroscience ; 210: 340-52, 2012 May 17.
Article in English | MEDLINE | ID: mdl-22441036

ABSTRACT

The objective of the present study was to assess the neuroprotective role of rutin (vitamin P) and delineate the mechanism of action. Recent evidence indicates that rutin exhibits antioxidant potential and protects the brain against various oxidative stressors. More precisely, the aim of the present study was to examine the modulating impacts of rutin against cognitive deficits and oxidative damage in intracerebroventricular-streptozotocin (ICV-STZ)-infused rats. Rats were injected bilaterally with ICV-STZ (3 mg/kg), whereas sham rats received the same volume of vehicle. After 2 weeks of streptozotocin (STZ) infusion, rats were tested for cognitive performance using Morris water maze tasks and thereafter euthanized for further biochemical, histopathological, and immunohistochemical studies. Rutin pretreatment (25 mg/kg, orally, once daily for 3 weeks) significantly attenuated thiobarbituric acid reactive substances (TBARS), activity of poly ADP-ribosyl polymerase, and nitrite level and decreased level of reduced glutathione (GSH) and activities of its dependent enzymes (glutathione peroxidase [GPx] and glutathione reductase [GR]) and catalase in the hippocampus of ICV-STZ rats. ICV-STZ rats showed significant cognitive deficits, which was improved significantly by rutin supplementation. The results indicate that rutin attenuates STZ-induced inflammation by reducing the expression of cyclooxygenase-2 (COX-2), glial fibrillary acidic protein (GFAP), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), nuclear factor-kB, and preventing the morphological changes in hippocampus. The study thereby suggests the effectiveness of rutin in preventing cognitive deficits and might be beneficial for the treatment of sporadic dementia of Alzheimer type (SDAT).


Subject(s)
Alzheimer Disease/metabolism , Antioxidants/pharmacology , Inflammation/metabolism , Oxidative Stress/drug effects , Rutin/pharmacology , Alzheimer Disease/drug therapy , Animals , Brain/drug effects , Brain/metabolism , Cognition Disorders/metabolism , Cognition Disorders/prevention & control , Disease Models, Animal , Fluorescent Antibody Technique , Immunohistochemistry , Male , Maze Learning/drug effects , Rats , Rats, Wistar
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