Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
Add more filters










Database
Publication year range
1.
Klin Mikrobiol Infekc Lek ; 28(2): 45-47, 2022 Jun.
Article in Czech | MEDLINE | ID: mdl-36546469

ABSTRACT

This is a case report of sepsis caused by the species Actinobacillus suis/equuli in a male agriculture worker that ended fatally. The article also contains information on identification and results of antibiotic susceptibility testing. This is a rare case of human infection and probably the first case of a human being infected by this species in the Czech Republic.


Subject(s)
Actinobacillus Infections , Actinobacillus equuli , Actinobacillus suis , Actinobacillus , Sepsis , Humans , Adult , Male , Sepsis/diagnosis
2.
Folia Microbiol (Praha) ; 67(1): 121-127, 2022 Feb.
Article in English | MEDLINE | ID: mdl-34590203

ABSTRACT

The isolation of Planococcus glaciei (designed strain CNCTC 7660) from blood of a patient with appendicitis is reported. Species P. glaciei (type strain CGMCC 1.6846 T) was for the first time identified as an environmental bacterium acquired from a glacier in China in 2009. To reveal the identity of the isolate CNCTC 7660, the 16S rDNA sequencing and the whole genome sequencing (Illumina MiSeq, Oxford Nanopore) were performed. The level of 16S rDNA gene sequencing similarity between CNCTC 7660 and CGMCC 1.6846 T was 99.55%. Phylogenetic analysis and average nucleotide analysis (ANI) based on the whole genome sequencing confirmed that the isolate CNCTC 7660 and CGMCC1.6846 T had ANI value above the taxonomic threshold for belonging to the same species (95%). The G + C content of CNCTC 7660 DNA was 46.8% (mol/mol). Except for the growth temperature, strains CGMCC1.6846 T and CNCTC 7660 were distinguished also biochemically. Due to the lack of information about the pathogenicity of P. glaciei, the possibility that it exerts pathogenicity in persons is suggested. But for understanding the nature of this species, further cases are needed.


Subject(s)
Fatty Acids , Bacterial Typing Techniques , Czech Republic , DNA, Bacterial/genetics , Fatty Acids/analysis , Humans , Nucleic Acid Hybridization , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA
4.
Syst Appl Microbiol ; 44(4): 126217, 2021 Jul.
Article in English | MEDLINE | ID: mdl-34107439

ABSTRACT

This study aimed to define the taxonomic position and structure of a novel, taxonomically unique group of 26 Acinetobacter strains, provisionally designated Taxon 24 (T24). The strains were recovered from soil and freshwater ecosystems (n = 21) or animals (n = 5) in Czechia, Scotland, Germany, the Netherlands and Turkey between 1993 and 2015. They were non-glucose-acidifying, nonhemolytic, nonproteolytic, growing at 32 °C and on acetate and ethanol as single carbon sources, but not on 4-hydroxybenzoate and mostly not at 37 °C. Their whole-genome sequences were 3.0-3.7 Mb in size, with GC contents of 39.8-41.3%. Based on core genome phylogenetic analysis, the 26 strains formed a distinct clade within the genus Acinetobacter, with strongly supported subclades termed T24A (n = 11), T24B (n = 8), T24C (n = 2), T24D (n = 3) and T24E (n = 2). The internal genomic ANIb values for these subclades were >94.8%, while the ANIb values between them were <92.5%. The results of MALDI-TOF MS-based analyses agreed with this classification. The five subclades differed from each other in the results of one to six carbon source assimilation tests. Given the genomic and phenotypic distinctness, internal coherence, numbers of available strains and geographically diverse origin of T24A and T24B, we propose the names Acinetobacter terrae sp. nov. and Acinetobacter terrestris sp. nov. for these two taxa, respectively. The type strains are ANC 4282v (= CCM 8986T = CCUG 73811T = CNCTC 8082T) and ANC 4471T (= CCM 8985T = CCUG 73812T = CNCTC 8093T), respectively. We conclude that these two species together with the other T24 strains represent a widely dispersed Acinetobacter clade primarily associated with terrestrial ecosystems.


Subject(s)
Acinetobacter , Phylogeny , Acinetobacter/classification , Animals , Bacterial Typing Techniques , Base Composition , Czech Republic , DNA, Bacterial/genetics , Ecosystem , Fresh Water/microbiology , Genes, Bacterial , Germany , Netherlands , Nucleic Acid Hybridization , RNA, Ribosomal, 16S/genetics , Scotland , Sequence Analysis, DNA , Soil Microbiology , Turkey
5.
Klin Mikrobiol Infekc Lek ; 27(4): 158-162, 2021 Dec.
Article in Czech | MEDLINE | ID: mdl-35220576

ABSTRACT

The article describes a case of a rare infection caused by Yersinia pseudotuberculosis in a five-year-old boy admitted to the hospital. The infection was manifested by the so-called right lower quadrant syndrome, or terminal ileitis. The Y. pseudotuberculosis strain was isolated from the patient's feces and its biochemical properties are reported. Confirmation was performed by the National Reference Laboratory for E. coli and Shigella. Since pseudotuberculosis is very rare in the Czech Republic, the authors would like to draw attention to this infection. Enlargement of lymph nodes in the right lower quadrant of the abdomen may suggest the infection caused by Y. pseudotuberculosis.


Subject(s)
Gastroenteritis , Yersinia pseudotuberculosis Infections , Yersinia pseudotuberculosis , Child, Preschool , Escherichia coli , Feces , Gastroenteritis/diagnosis , Humans , Male
6.
Klin Mikrobiol Infekc Lek ; 26(1): 12-17, 2020 Mar.
Article in Czech | MEDLINE | ID: mdl-33326590

ABSTRACT

Actinomyces urogenitalis is most commonly associated with the human genitourinary system, often only as the resident flora. Outside the genitourinary tract, A. urogenitalis is isolated rather sporadically. Presented are two brief case reports of human infections outside the genitourinary tract as well as experiences with microbiological identification of this actinomycete. Antibiotic susceptibility testing of actinomycetes is focused especially on their resistance to lincosamides and fluoroquinolones. The etiological relationship with the patients' clinical problems was not investigated. Previously reported cases of infections outside the genitourinary tract are also mentioned in the article. The article may aid in expanding the knowledge of the occurrence, diagnosis and susceptibility of A. urogenitalis to antibiotics, particularly in rarely reported extra-genitourinary infections caused by this species. Accurate species identification in routine laboratory practice is important both for determination of the etiological role of the microorganism and for more precise selection of empirical antibiotic therapy.


Subject(s)
Actinomyces/drug effects , Anti-Bacterial Agents/pharmacology , Gram-Positive Bacterial Infections/microbiology , Actinomyces/isolation & purification , Drug Resistance, Bacterial , Gram-Positive Bacterial Infections/drug therapy , Humans , Microbial Sensitivity Tests
7.
Klin Mikrobiol Infekc Lek ; 26(3): 106-110, 2020 Sep.
Article in Czech | MEDLINE | ID: mdl-33418599

ABSTRACT

The authors present three case reports of bacteremia with very rare microorganisms being isolated in the patients. Each brief case report is accompanied by etiological considerations related to the diagnosis and clinical condition of the patient. Routine microbiology tests were performed by examining the biochemical properties using commercial kits. Two methods were used to determine the microorganism species in the State Institute of Public Health in Prague: MALDI-TOF MS and 16S rDNA sequencing. Antibiotic susceptibility was assessed by the disk diffusion test; minimum inhibitory concentrations were determined using broth microdilution and the gradient diffusion method (E tests). This is probably the first reported case of Stenotrophomonas acidaminiphila isolated from human clinical specimens.


Subject(s)
Bacteremia , Stenotrophomonas , Humans , Microbial Sensitivity Tests , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
8.
Folia Microbiol (Praha) ; 64(2): 231-236, 2019 Mar.
Article in English | MEDLINE | ID: mdl-30238302

ABSTRACT

A group of 59 putative strains of Staphylococcus intermedius/Staphylococcus pseudintermedius deposited in the Czech National Collection of Type Cultures (CNCTC, National Institute for Public Health, Prague, Czech Republic) and the National Reference Laboratory for Staphylococci (NRL for Staphylococci, National Institute for Public Health, Prague, Czech Republic) was reclassified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There the biggest human collection of S. pseudintermedius in Europe was analysed; 44 samples (75%) were of human origin. Twenty-two percent (n = 13) of the strains were isolated from animals, and two staphylococci were of unknown origin. This study revealed the prevalence of Staphylococcus pseudintermedius (94%, n = 53) vs. Staphylococcus intermedius (6%, n = 6) in the collection of human and veterinary staphylococci after reclassification. Results of PCR-RFLP analysis were verified by comparison with a repetitive element sequence-based polymerase chain reaction (Rep-PCR) analysis on 26 (44%) randomly selected strains. Due to a low-resolution ability of PCR-RFLP to separate Staphylococcus intermedius from Staphylococcus delphini, four isolates of Staphylococcus intermedius were biochemically verified further to exclude the presence of Staphylococcus delphini in the collection. Our results indicate that S. intermedius and S. pseudintermedius have occurred independently over an age-long period of their co-evolution.


Subject(s)
Biological Evolution , Staphylococcal Infections/microbiology , Staphylococcal Infections/veterinary , Staphylococcus/classification , Animals , Bacterial Proteins/genetics , Bacteriological Techniques , DNA, Bacterial/genetics , Humans , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Repetitive Sequences, Nucleic Acid/genetics , Sequence Analysis, DNA , Staphylococcus/genetics , Staphylococcus/isolation & purification , Staphylococcus intermedius/classification , Staphylococcus intermedius/genetics , Staphylococcus intermedius/isolation & purification
SELECTION OF CITATIONS
SEARCH DETAIL
...