[Detection of influenza virus infections by molecular and immunofluorescence methods]. / Influenza virüs enfeksiyonlarinin moleküler ve immün floresan yöntemlerle saptanmasi.

Influenza virus infections are extremely important for human health due to the occurence of seasonal epidemics and pandemics worldwide. Influenza is associated with high morbidity and may result in serious complications such as life threatening viral or bacterial pneumonia. Especially, young children, older adults, patients with chronic diseases such as heart, lung, kidney, and diabetes and immunosuppressed people are at higher risk for complications and death from influenza virus infections. The aim of this study was to determine the incidence of influenza type A and B virus infections and influenza A virus subtypes in hospitalized patients with respiratory tract infections by real-time reverse transcriptase-polymerase chain reaction (RT-PCR, Sacace, Italy), conventional RT-PCR and direct immunofluorescence antibody (DFA, Argene SA, France) tests. Nasopharyngeal swab specimens were collected from a total of 476 patients with respiratory tract symptoms by using flocked swabs (Copan Diagnostics, Italy) between 1 April 2012 and 31 December 2013. Influenza A virus was detected in 20.5% (98/476) and influenza B virus in 3.3% (16/476) of the cases by real-time RT-PCR test. During the study period, 63.3% of 98 influenza virus isolates were found as influenza A(H1N1)pdm09 and 36.7% were influenza A(H3N2) subtypes. Influenza A (H1N1) pdm09 subtype was observed in 12 cases in January 2013 and influenza A(H3N2) subtype was observed in 11 cases in December 2013 as the highest values. When the real-time RT-PCR test was regarded as the reference test, the sensitivities of DFA test for influenza A and B and conventional RT-PCR test with WHO primers (M30F2/08 and M264R3/08) for influenza A were detected as 72.4%, 75%, 96% and the specificities were detected as 99.2%, 99.5% and 100%, respectively. In conclusion, influenza A virus infection was detected rather high with a rate of 20.5% in the study group. The monitoring of influenza virus types and subtypes is required for the evaluation of influenza vaccine strains and circulating influenza viruses and for the identification of subtypes with pandemic potential. Planning for appropriate antiviral therapy using real-time RT-PCR in the early diagnosis of influenza virus infections will significantly contribute to the management of the patient's treatment. Thus, unnecessary drug use will be prevented and controlled with effective treatment of the disease at the time of infection.

Nasal cavity volume changes after rapid maxillary expansion in adolescents evaluated with 3-dimensional simulation and modeling programs.

INTRODUCTION: The purpose of this study was to evaluate the effects of rapid maxillary expansion on nasal cavity volume by using 3-dimensional simulation and modeling programs. METHODS: The study group consisted of 15 patients (9 boys, 6 girls; mean age, 13.86 years) with maxillary constriction. Computed tomography scans were obtained before treatment and 6 months after the end of expansion. All computed tomography data were transferred to a computer, and the nasal cavity and maxillary teeth were segmented by using the Mimics and Simplant Ortho software programs (both, Materialise, Leuven, Belgium). Paired samples t tests were used to compare pretreatment and posttreatment nasal cavity volumes and maxillary areas. Data analysis was performed by using the software program SPSS for Windows (version 15.00; SPSS, Chicago, Ill). RESULTS: Rapid maxillary expansion treatment induced significant increases in nasal cavity volume (P ≤ 0.001) and maxillary transverse dimensions (P ≤ 0.001). CONCLUSIONS: Both anterior-to-posterior and coronal-to-cranial expansions were observed after rapid maxillary expansion treatment, with the direction of expansion most likely affected by resistance from the cranial bones.

Tongue movements in patients with skeletal Class III malocclusions evaluated with real-time balanced turbo field echo cine magnetic resonance imaging.

INTRODUCTION: The aim of this study was to evaluate the position and movements of the tongue in patients with skeletal Class III malocclusion. METHODS: Sixty-six patients (31 male, 35 female) with Class III malocclusion were divided into 3 groups according to cephalometric analysis. The first group comprised 23 patients (13 male, 10 female) with mandibular prognathism, the second group comprised 21 patients (9 male, 12 female) with maxillary retrognathism, and the third group comprised 22 patients (9 male, 13 female) with both maxillary retrognathism and mandibular prognathism. Twenty-two skeletal Class I patients (10 male, 12 female) were also included as the control group. RESULTS: Dentofacial morphology affects the position and the movements of the tongue during deglutition. Contact of the anterior portion of the tongue with the rugae area of the hard palate decreased in the Class III malocclusion groups. The posterior portion of the dorsal tongue was positioned more inferiorly, and the root of the tongue was positioned more inferiorly and anteriorly in patients with Class III malocclusion than in the control group. The tip of the tongue was also in a more anterior position in the Class III groups. When the deglutition stages were evaluated, we observed that the manner of bolus transfer was different in patients with skeletal Class III malocclusion than in those with skeletal Class I malocclusion. CONCLUSIONS: Tongue posture is affected by dentofacial structures, and adaptive changes occur in the tip, dorsum, and root of the tongue. Deglutitive tongue movements in patients with skeletal Class III malocclusion are also different from those with skeletal Class I malocclusion.

Tongue movements in patients with skeletal Class II malocclusion evaluated with real-time balanced turbo field echo cine magnetic resonance imaging.

INTRODUCTION: The aim of this study was to evaluate the deglutitive tongue movements in patients with skeletal Class II malocclusion. METHODS: Fifty-nine patients (26 male, 33 female) with skeletal Class II relationship were divided into 3 groups according to cephalometric analysis. Group 1 (n = 19) had mandibular retrognathism, group 2 (n = 20) had maxillary prognathism, and group 3 (n = 20) had both mandibular retrognathism and maxillary prognathism. Twenty-two skeletal Class I patients (10 male, 12 female) were also included as the controls. RESULTS: In the mandibular retrusion group, the posterior portion of the dorsal tongue moved downward at stage 2 and upward at stage 3; the root of the dorsal tongue was in an inferior and anterior position at stage 2. In patients with both mandibular retrognathism and maxillary prognathism, the middle portion of the dorsal tongue was positioned superiorly at stage 3 relative to stage 1; the tongue tip was retruded at stage 3 relative to stages 1 and 2. In the control group, the middle portion of dorsal tongue was positioned superiorly at stage 3 relative to stages 1 and 2; the posterior portion of the tongue moved upward at stage 2 and downward at stage 3, and tongue-tip retrusion was observed at stage 2 relative to stage 1. Contact of the anterior portion of the tongue with the rugae area of the hard palate decreased in the Class II malocclusion groups relative to the control group. The middle portion of the dorsal tongue was positioned more superiorly in patients with Class II malocclusion during all stages of deglutition. The root of the tongue was more inferior and anterior, and the tongue tip was retruded in patients with Class II malocclusion compared with the control group. The posterior portion of the dorsal tongue was more inferiorly positioned in patients with mandibular retrusion than in the other Class II groups or the controls. In the third stage of deglutition, this portion of the tongue had a superior position in groups 2 and 3 relative to the control group. CONCLUSIONS: Dentofacial morphology affects the position and movements of the tongue during deglutition, and adaptive changes occur in the tip, dorsum, and root of the tongue. Deglutitive tongue movements in patients with a skeletal Class II relationship are different from those with a skeletal Class I relationship.

Effect of antibacterial monomer-containing adhesive on enamel demineralization around orthodontic brackets: an in-vivo study.

INTRODUCTION: The aims of this study were to evaluate the effect of an antibacterial monomer-containing self-etching adhesive in reducing enamel demineralization around orthodontic brackets in vivo and to compare it with the conventional adhesive system quantitatively. METHODS: Fourteen orthodontic patients were randomly divided into 2 equal groups; they received brackets fitted to all their teeth, bonded with either Clearfil Protect Bond (Kuraray Medical, Okayama, Japan) (experimental group) or Transbond XT (3M Unitek, Monrovia, Calif) (control group). Block randomization to obtain equal numbers in each group was used. After 30 days, all first premolars were extracted with orthodontic indications and longitudinally sectioned. Demineralization was assessed by cross-sectional microhardness. Determinations were made at the bracket edge cementing limits and at occlusal and cervical points 100 and 200 µm away from the edge. In all of these positions, 6 indentations were made at depths of 10 to 90 µm from the enamel surface. Analysis of variance (ANOVA) and the Tukey post-hoc test were used. The statistical significance level was set at P <0.05. RESULTS: ANOVA showed statistically significant differences for adhesive type, position, depth, and their interactions (P <0.05). The multiple comparison test showed that the antibacterial monomer-containing adhesive was significantly more efficient than the conventional adhesive system, reducing enamel demineralization in almost all evaluations (P <0.05). CONCLUSIONS: The results indicated that using antibacterial monomer-containing adhesive for bonding orthodontic brackets successfully inhibited caries in vivo. This cariostatic effect was localized at the area around the brackets and was significant after 30 days.

Does a chitosan-containing dentifrice prevent demineralization around orthodontic brackets?

OBJECTIVE: To test the null hypothesis that there is no significant difference between the chitosan-containing and conventional nonfluoridated dentifrices in inhibition of enamel demineralization around orthodontic brackets. MATERIALS AND METHODS: Sixteen orthodontic patients who were scheduled to have extraction of four first premolars for orthodontic reasons were divided into two groups after the power of the study was estimated. Patients in the experimental group were instructed to use chitosan-containing dentifrice (AloeDent), and patients in the control group were instructed to use nonfluoridated dentifrice (Sensodyne Mint). After 60 days, the teeth were extracted and longitudinally sectioned. The demineralization was assessed by cross-sectional microhardness. The determinations were made at the bracket edge cementing limits and at occlusal and cervical points, 100 µm and 200 µm away from the edge. In all these positions, indentations were made at depths of 10, 20, 30, 50, 70, and 90 µm from the enamel surface. Analysis of variance (ANOVA) and Tukey test were used for statistical evaluation at P < .05 level. RESULTS: ANOVA showed statistically significant differences for the factors of dentifrice type, position, and depth (P  =  .000). Statistically significant differences for microhardness values between two tested dentifrices were observed up to 20 µm of depth from the enamel surface (P < .05). Lower microhardness values were found for nonfluoridated dentifrice. Significant microhardness differences were also determined between materials at occlusal and cervical 0 µm positions (P < .05). At these positions, chitosan-containing dentifrice showed lower demineralization than the control. CONCLUSION: Chitosan-containing dentifrice may reduce the enamel decalcification found in patients with poor oral hygiene. The null hypothesis is rejected.

Amorphous calcium phosphate-containing orthodontic composites. Do they prevent demineralisation around orthodontic brackets?

BACKGROUND: A preliminary study using laser fluorescence suggested that amorphous phosphate-containing orthodontic composites may prevent demineralisation around bonded orthodontic brackets. OBJECTIVE: To compare the microhardness of the enamel around brackets bonded with an amorphous calcium phosphate-containing orthodontic composite (ACP-containing) with the microhardness of the enamel around brackets bonded with a conventional composite resin. METHODS: Forty extracted upper premolars were used. Orthodontic brackets were bonded to the teeth with either an ACP-containing composite resin (N = 20) or a conventional composite resin (N = 20). The latter were used as the control. The crowns of all teeth were painted with an acid resistant varnish, leaving a 2 mm ring of exposed enamel around the brackets. The teeth were then subjected to a daily cycle of demineralisation for 6 hours and remineralisation for 18 hours for 21 days. Each tooth was sectioned and the microhardness of the enamel determined 25, 50, 75, 100 and 150 microm from the surface. RESULTS: The enamel was significantly harder 25 microm (p = 0.000) and 50 microm (p = 0.001) from the enamel surface in the teeth with brackets bonded with the ACP-containing composite resin as compared with the control teeth. CONCLUSION: ACP-containing orthodontic composite resins may reduce the enamel decalcification found in patients with poor oral hygiene.

Demineralization properties of newly erupted and mature premolars around orthodontic brackets: an in-vivo study.

INTRODUCTION: The aims of this study were to evaluate the in-vivo reaction of newly erupted enamel to demineralization around orthodontic brackets and to compare it with that of mature enamel. METHODS: Thirteen orthodontic patients scheduled to have 4 first premolars extracted for orthodontic reasons were divided into 2 groups. Group 1 included 7 younger patients with newly erupted teeth (4 boys, 3 girls; mean age, 11.21 +/- 1.12 years; range, 11-13 years). Group 2 contained 6 adults with mature teeth (5 men, 1 woman; mean age, 34.64 +/- 4.01 years; range, 25-41 years). Brackets were placed, and, 30 days later, the teeth were extracted. These teeth were longitudinally sectioned, and demineralization was assessed by cross-sectional microhardness. Determinations were made at the bracket-edge composite limits and at occlusal and cervical points 100 mum away. Evaluations under the brackets and at the lingual surfaces were made as controls. In all these positions, 6 indentations were made at depths from 10 to 90 mum from the enamel surface. Analysis of variance (ANOVA) and Tukey tests were used for statistical evaluation at the P <0.05 level. RESULTS: ANOVA showed statistically significant differences for tooth type, position, depth, and their interactions (P <0.05), except the tooth type and position interaction. The multiple comparison test showed less demineralization in the enamel around orthodontic brackets bonded to mature teeth campared with newly erupted teeth (P <0.05). CONCLUSIONS: During the 30-day study period, the tooth enamel in the adult orthodontic patients was more resistant to demineralization than that of the younger patients.

Effect of ED-71, a New Active Vitamin D Analog, on Bone Formation in an Orthopedically Expanded Suture in Rats. A Histomorphometric Study.

OBJECTIVES: The aim of this experimental study was to evaluate the effects of ED-71, a new active vitamin D analog, on bone regeneration in response to expansion of the mid-palatal suture, in rats, histomorphometrically. METHODS: Sixteen male 50-60 days old Wistar rats were separated into two equal groups (control and experimental). Both groups were subjected to expansion, and 30 grams of force was applied to the maxillary incisors with a helical-spring. Experimental group was treated with single-dose ED-71 (0.8 mug/kg body weight) in the mid-palatal suture locally and eight control animals received vehicle solution. Bone regeneration in the mid-palatal suture was evaluated by bone histomorphometric method and mineralized area (Md.Ar), fibrosis area (Fb.Ar), mineralized area/fibrosis area (Md.Ar/Fb.Ar), bone area (B.Ar) and osteoblast number (N.Ob) parameters were evaluated. Mann Whitney-U test was used for statistical evaluation at P<.05 level. RESULTS: Statistical analysis showed significant differences between groups for all investigated histomorphometric parameters. Md.Ar (P<.001), Md.Ar/Fb.Ar (P<.001), B.Ar (P<.01) and N.Ob (P<.001) parameters were significantly increased and Fb.Ar (P<.001) measurement was significantly decreased in experimental group. ED-71 group with a mean of 24.55+/-6.47 showed statistically higher N.Ob than the control group (mean N.Ob: 12.82+/-5.81). CONCLUSIONS: ED-71 has positive effects on early phase of bone regeneration in the mid-palatal suture in response to expansion and may be beneficial in routine maxillary expansion procedures.

Efficiency of amorphous calcium phosphate-containing orthodontic composite and resin modified glass ionomer on demineralization evaluated by a new laser fluorescence device.

OBJECTIVES: The aim of this in vitro study was to compare the efficacy of Amorphous Calcium Phosphate (ACP)-containing orthodontic composite and resin-modified glass ionomer cement (RMGIC) on enamel demineralization adjacent to orthodontic brackets evaluated by a new laser fluorescence device. METHODS: Sixty extracted maxillary premolars were used in the present study. Twenty orthodontic brackets were bonded with ACP-containing orthodontic adhesive (Aegis-Ortho), 20 were bonded with RMGIC (Fuji Ortho LC) and 20 were bonded with Transbond XT composite as the control. All samples were then cycled for 21 days through a daily procedure of demineralization for 6 hours and remineralization for 17 hours. After this procedure, demineralization evaluations were undertaken by a pen-type laser fluorescence device (DIAGNO-dent Pen). Analysis of variance (ANOVA) and Tukey test was used for statistical evaluation, at P<.05 level. RESULTS: According to ANOVA, significant demineralization variations (DeltaD) were determined among groups (F=6.650; P<.01). The ACP-containing composite showed the lowest (mean: 8.98+/-2.38) and the control composite showed the highest (mean:12.15+/-3.83) DeltaD, during 21 days demineralization process (P<.01). Significant difference was also observed between the DeltaD scores of the RMGIC (mean: 9.24+/-2.73) and control (P<.05). No significant differences was found in preventive effects of ACP-containing composite and RMGIC (P<.05) against demineralization. CONCLUSIONS: The use of both ACP-containing orthodontic composite and RMGIC should be recommended for any at-risk orthodontic patient to provide preventive actions and potentially remineralize subclinical enamel demineralization.

Effects of hyperbaric oxygen during experimental tooth movement.

OBJECTIVE: To determine the effects of hyperbaric oxygen (HBO) on bone remodeling during orthodontic tooth movement. MATERIALS AND METHODS: Twenty-four male, adult Sprague Dawley rats were randomly divided into two groups. HBO was administered in the first group, and the second group served as a control. The mandibular first molars were moved mesially by means of Ni-Ti closed coil springs in all groups. RESULTS: Results were evaluated histomorphometrically and the parameters of trabecular bone volume (BV/TV), trabecular bone number (Tr.N), and trabecular separation (Tr.Sep) were evaluated at the interradicular bone area of the mandibular first molars. Increases in BV/TV and Tr.N and decreases in Tr.Sep revealed the osteoblastic activity of HBO. HBO application caused an increase in bone apposition and osteoblastic activity or a decrease in osteoclastic activity. CONCLUSIONS: HBO enhanced the bone formation during experimental tooth movement. Therefore, the findings of this study support our hypothesis that osteoblastic activity might be modulated by changes in the environmental oxygen tension.

Forsus Nitinol Flat Spring and Jasper Jumper corrections of Class II division 1 malocclusions.

OBJECTIVE: To compare the effects of Forsus Nitinol Flat Spring (FNFS) and Jasper Jumper (JJ) in the correction of Class II division I malocclusions. MATERIALS AND METHODS: Our research was conducted on 48 adolescents, who had a normal or horizontal growth pattern and retrognathic mandible. The patients were divided into three equal groups randomly. First group was treated with FNFS, and the second group was treated with JJ appliances, whereas the third group was the control group. Lateral cephalograms and study models were obtained after the leveling phase and at time of the removal of the appliances. RESULTS: Cephalometric analysis revealed that both the appliances stimulated mandibular growth, increased the anterior face height because of the lower face, and elongated the posterior face height because of the growth of temporomandibular joint. Maxillary central incisors were extruded, retruded, and distally tipped. Contrarily, intrusion, protrusion, and labial tipping were observed in the mandibular central incisors. Distal movement and intrusion of the maxillary first molars and mesial movement and extrusion of the mandibular first molars were the other dental alterations. Overjet and overbite were decreased, and a Class I molar relationship and improvement in the profile were attained in both treatment groups. Cast model analysis showed expansion in the maxillary and mandibular dental arches. CONCLUSIONS: Both the appliances were effective in the treatment of Class II malocclusion and revealed nearly same alterations in the skeletal, dental, and soft tissue parameters.

Effects of a segmented removable appliance in molar distalization.

The aim of the present investigation was to evaluate the skeletal and dentoalveolar treatment effects of a segmented removable appliance [removable molar distalizer (RMD)] for molar distalization. The study was conducted on 28 patients (12 females and 16 males), with a mean age of 11.8 years. All presented with a skeletal Class I malocclusion and a bilateral dental Class II molar relationship. The pre- and post-distalization records included lateral head films, study models and standard photographs. The findings were evaluated with a paired samples t-test. The average maxillary first molar distalization with the RMD was 3.98 mm, with 4.61 degrees of distal tipping. The maxillary second premolars drifted distally 2.13 mm on average with 1.54 degrees of distal tipping, while the maxillary first premolars showed 1.23 mm of mesial movement and 1.98 degrees of mesial tipping. The incisors protruded 1.09 mm with 1.27 degrees of labial tipping. The RMD was effective in distal molar movement and all patients attained a bilateral Class I molar relationship in an average period of 4.5 months. Hygiene problems and mucosal irritations, frequently found with fixed intraoral distalization techniques, were not observed during the distalization period.

Effects of PGI2 and TxA2 analogs and inhibitors in orthodontic tooth movement.

This study evaluates the effects of prostacyclin (PGI2) and thromboxane A2 (TxA2) in orthodontic tooth movement and osteoclastic activity in rats. The study sample consisted of 150 male Sprague-Dawley rats. The rats were randomly divided into five equal groups, and each group was again equally divided into three subgroups (SGs). Twenty grams of reciprocal force was applied to maxillary incisors of the rats with a spring bent from 0.35 mm stainless steel wire, except for the rats in the last SG. Iloprost (PGI2 analog), indomethacin (PGI2 inhibitor), U 46619 (TxA2 analog), and imidazole (TxA2 inhibitor) were dissolved in 0.9% NaCl (saline solution), and each material was prepared in three different concentrations (10(-4), 10(-5), and 10(-6) M/L). Iloprost was administered (20 microL/12 hours) in the first three SGs with the sequence of 10(-4), 10(-5), and 10(-6) M/L. Indomethacin, U 46619, and imidazole were administered in the next nine SGs with the same sequence and dose. In SG 13, 0.9% NaCl solution was administered (20 microL/ 12 hours) to the rats together with orthodontic force. Only orthodontic force was not used in SG 14, and neither any solution nor orthodontic force was used in the last SG. The rats were sacrificed on the fifth day of the experiment, premaxillae were dissected, and cross samples were taken. The results showed that PGI2 and TxA2 analogs increased the number of multinuclear osteoclasts, osteoclastic bone resorption, and rate of orthodontic tooth movement.