ABSTRACT
The attraction of bacteria commonly found in the respiratory tract in cystic fibrosis to glycoprotein at pH 7 has been studied. The effect of washing on the removal of bacteria from the glycoprotein has also been investigated, and the value of doing both washed and unwashed sputum cultures is discussed.
Subject(s)
Bacteria/isolation & purification , Cystic Fibrosis/microbiology , Glycoproteins , Respiratory System/microbiology , Bacteriological Techniques , Haemophilus influenzae/isolation & purification , Humans , Hydrogen-Ion Concentration , Klebsiella/isolation & purification , Proteus/isolation & purification , Pseudomonas aeruginosa/isolation & purification , Sputum/microbiology , Staphylococcus/isolation & purification , Streptococcus/isolation & purificationABSTRACT
The differential attraction in vitro of antibiotics to blood lymphocytes and salivary glycoprotein was studied in an attempt to determine the distribution of antibiotics in sputum. Results from studies in vivo on sputum antibiotic levels are also presented.
Subject(s)
Anti-Bacterial Agents/analysis , Glycoproteins/analysis , Lymphocytes/analysis , Sputum/analysis , Cloxacillin/analysis , Cystic Fibrosis/metabolism , Gentamicins/analysis , Penicillins/analysis , Saliva/analysis , Tetracycline/analysisABSTRACT
Staphylococci split a wide range of lipid substrates by production of an enzyme complex with two main components (i) a lipase acting optimally on fat-soluble glycerides, and (ii) an esterase acting optimally on water-soluble esters. The action is dependent upon carbon chain length, interfacial dispersion, solubility, and pH of substrate and end products. The esterase is less susceptible to organophosphorus inhibitors than mammalian esterases. There is no apparent correlation between lipolysis and markers of pathogenicity such as production of coagulase and toxin, but the possession of a flexible lipolytic mechanism might account for the persistence of staphylococci in the fatty secretions of mammalian skin.
Subject(s)
Esterases/metabolism , Lipase/metabolism , Staphylococcus/enzymology , Esterases/analysis , Esterases/antagonists & inhibitors , Glycerides/metabolism , Hydrogen-Ion Concentration , Kinetics , ManometrySubject(s)
Anti-Bacterial Agents/analysis , Cystic Fibrosis/drug therapy , Sputum/analysis , Acetylcysteine/pharmacology , Aerosols , Ampicillin/analysis , Ampicillin/blood , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/blood , Biological Assay , Cephaloridine/analysis , Cephaloridine/blood , Cloxacillin/analysis , Cloxacillin/blood , Cystic Fibrosis/complications , Erythromycin/analysis , Erythromycin/blood , Gentamicins/analysis , Gentamicins/blood , Humans , Infection Control , Lincomycin/analysis , Lincomycin/blood , Methicillin/analysis , Methicillin/blood , Molecular Weight , Novobiocin/analysis , Novobiocin/blood , Sulfonamides/analysis , Sulfonamides/bloodSubject(s)
Cystic Fibrosis/diagnosis , Infant, Newborn , Saliva/analysis , Sodium/analysis , Humans , Infant , Parotid Gland/metabolismSubject(s)
Cystic Fibrosis/diagnosis , Parotid Gland , Saliva/analysis , Sodium/analysis , Adolescent , Adult , Child , Child, Preschool , Humans , Infant , Middle AgedABSTRACT
A study has been made on the penetration values in vitro of antibiotics through hog gastric mucin at pH 7.4. Determinations have also been done on the binding of antibiotics to mucus and human plasma. The mucolytic agent N-acetyl cysteine has been studied in regard to its effect on antibiotics penetrating mucus and for any possible inactivation of antibiotics.
