ABSTRACT
The LD50 determination is the main way to measure the acute toxicity of all types of substances. At the present time, however, there is increasing opposition to the use of living animals in research and testing activities from animal rights groups as well as some scientists. Nevertheless, the need to have a tool for estimating the potential toxicity of new compounds for human consumption has encouraged the development of alternative methods. Under adequate conditions, the partitioning in micellar liquid chromatography can describe the drug biopartitioning. We have named this chromatographic system biopartitioning micellar chromatography (BMC). In this paper, an LD50 QRAR model developed for psychotropic drugs from their retention data in BMC, is described. The model's ability to predict new psychotropic drug toxicity is statistically proved.
Subject(s)
Chromatography, Liquid/methods , Psychotropic Drugs/toxicity , Evaluation Studies as Topic , Lethal Dose 50 , Micelles , Spectrophotometry, UltravioletABSTRACT
A flow injection-spectrophotometric determination of paracetamol is reported. The procedure is based on the oxidation of the analyte with potassium hexacyanoferrate(III) previously retained in an anionic exchange column and the reaction of the N-(hydroxyphenyl)-p-benzoquinonimine so produced with phenol. The oxidation is carried out at room temperature and in aqueous ammoniacal solution. Concentrations of paracetamol in the 0.20-20 ppm range are determined with relative standard deviations (RSD) of 0.6% (n = 40) at an injection rate of 42 samples h-1. The influence of foreign species on the assay and its application to the determination of paracetamol in several pharmaceutical formulations are reported.