ABSTRACT
The radiation-induced mortality together with the loss of body-weight and development of radiation sickness was studied in young adult Swiss albino mice after exposure to 1500 R of 60Co gamma-radiation in the presence and absence of 2-mercaptopropionyl-glycine (MGP), and results from both the groups were compared. It was found that the time of onset of radiation sickness was delayed in the drug-treated group, the mean survival time was also increased to 6 days as compared to 4.5 days in the untreated control group. These changes showed a direct correlation with the body-weight changes as the loss of weight in the drug-treated group was less marked and more gradual than in the untreated controls.
Subject(s)
Amino Acids, Sulfur/pharmacology , Radiation-Protective Agents/pharmacology , Tiopronin/pharmacology , Animals , Body Weight , Mice , Radiation Dosage , Radiation Injuries/prevention & controlABSTRACT
The behaviour of crypt cell population in mouse jejunum under continuous beta irradiation from tritiated water (HTO) has been studied. Adult mice were maintained on tritiated drinking water of the activity of 1.25 muCi/ml, after priming injection. The crypts were studied at 1, 5, 7, 15 and 30 days after the initiation of treatment. It is observed that there is a partial recovery in proliferative activity after the first day of the treatment. Again there is a decrease in the crypt cells on the 7th day, after which this population appears to achieve a near-steady-state level at about 8% below normal at the last interval studied. Crypt cell population and mitotic figures showed a simultaneous dip and recovery, while dead cells showed inverse relationship.
Subject(s)
Cell Division/radiation effects , Jejunum/radiation effects , Animals , Intestinal Mucosa/radiation effects , Mice , Mitosis , Time Factors , Tritium , WaterABSTRACT
Experiments were undertaken to study the goblet cell changes in the jejunum of mice irradiated in the presence and absence of the protective drug 2-mercaptopropionylglycine (MPG). One group of adult Swiss albino mice of 6 to 8 weeks was injected with the drug (MPG) intraperitoneally and a second group was injected with distilled water in the same manner and served as a control. 15-30 minutes after injection animals from both the groups were exposed to a Co-60 source to give a total dose of 1000 R at the rate of 25 R/min. Three animals from each group were sacrificed at different successive intervals. 5 micron paraffin sections of the jejunum were prepared and goblet cells were counted in the crypt and villus region. The results from the two groups were compared. The results from MPG treated mice indicated that there was a protection from the radiation-induced changes. The drug accelerated the regeneration process leading to the restoration of normal number of goblet cells by the last interval studies.
Subject(s)
Amino Acids, Sulfur/pharmacology , Jejunum/radiation effects , Radiation-Protective Agents/pharmacology , Tiopronin/pharmacology , Animals , Intestinal Mucosa/radiation effects , Mice , Radiation Dosage , Regeneration/drug effectsABSTRACT
The radiosensitivity of primary spermatocytes in pachytene stage was estimated by counting the number of spermatids in the testes of control and MPG-treated mouse after exposure to 500, 1000 and 1500 R of Co60 gamma-rays. For this purpose, control and MPG-treated mice were killed 5 days after irradiation and countings of spermatids was made in stages I and II of the tubules. It has been observed that, although there was a death of primary spermatocytes in irradiated MPG-protected groups, quantitatively significant protection was afforded by this drug at all the 3 dose-levels studied.
