ABSTRACT
The layer-by-layer (LbL) assembly technique has shown excellent potential in tissue engineering applications. The technique is mainly based on electrostatic attraction and involves the sequential adsorption of oppositely charged electrolyte complexes onto a substrate, resulting in uniform single layers that can be rapidly deposited to form nanolayer films. LbL has attracted significant attention as a coating technique due to it being a convenient and affordable fabrication method capable of achieving a wide range of biomaterial coatings while keeping the main biofunctionality of the substrate materials. One promising application is the use of nanolayer films fabricated by LbL assembly in the development of 3-dimensional (3D) bone scaffolds for bone repair and regeneration. Due to their versatility, nanoscale films offer an exciting opportunity for tailoring surface and bulk property modification of implants for osseous defect therapies. This review article discusses the state of the art of the LbL assembly technique, and the properties and functions of LbL-assembled films for engineered bone scaffold application, combination of multilayers for multifunctional coatings and recent advancements in the application of LbL assembly in bone tissue engineering. The recent decade has seen tremendous advances in the promising developments of LbL film systems and their impact on cell interaction and tissue repair. A deep understanding of the cell behaviour and biomaterial interaction for the further development of new generations of LbL films for tissue engineering are the most important targets for biomaterial research in the field. While there is still much to learn about the biological and physicochemical interactions at the interface of nano-surface coated scaffolds and biological systems, we provide a conceptual review to further progress in the LbL approach to 3D bone scaffold materials and inform the future of LbL development in bone tissue engineering.
Subject(s)
Biocompatible Materials , Tissue Engineering , Adsorption , Bone and Bones , Tissue Engineering/methodsABSTRACT
In this study, poly(lactic-co-glycolic acid) (PLGA)-gelatin scaffolds were fabricated using the freeze-casting technique. Polydopamine (PDA) coating was applied on the surface of scaffolds to enhance the hydrophilicity, bioactivity, and cellular behavior of the composite constructs. Further, the synergistic effect of PDA coating and lamellar microstructure of scaffolds was evaluated on the promotion of properties. Based on morphological observations, freeze-casting constructs showed lamellar pore channels while the uniformity and pore size were slightly affected by deposition of PDA. The hydrophilicity and swelling capacity of the scaffolds were assessed using contact angle measurement and phosphate buffered saline absorption ratio. The results indicated a significant increment in water-matrix interactions following surface modification. The evaluation of the biodegradation ratio revealed the higher degree of degradation in PDA-coated samples owing to the presence of hydrophilic functional groups in the chemical structure of PDA. On the other hand, the bioactivity potential of PDA in the simulated body fluid solution confirmed the possibility of using coated constructs as a bone reconstructive substitute. The improvement of cellular attachment and filopodia formation in PDA-contained matrixes was the other benefit of the coating process. Furthermore, cellular proliferation and ALP activity were enhanced after PDA coating. The suggested PDA-coated PLGA-gelatin scaffolds can be applied in bone tissue regeneration.
