The original Ivor Lewis two stage esophagectomy revisited in the era of minimally invasive surgery.

BACKGROUND: Esophagectomy has high cardiac and pulmonary complication rates that can reach 43% and 58% respectively. The original Ivor Lewis esophagectomy was a two-stage procedure. We revisited this procedure using a hybrid minimally-invasive approach. METHODS: Thirty-five consecutive patients with esophageal cancer were operated on over an eight-year period. The first stage used laparoscopic mobilization of the stomach, while the second stage used open thoracotomy. Six patients were aborted due to unresectable disease. RESULTS: Twenty-nine patients were studied. The mean operative times for stage-one and stage-two were 108 ±â€¯18 and 226 ±â€¯63 min respectively. All patients were extubated in the operating room. One (3.4%) patients had cardiac complication and one (3.4%) patient had pulmonary complication. CONCLUSION: Metachronous hybrid two-stage esophagectomy was associated with a low rate of cardio-pulmonary complications. It may be considered as an alternative to the one-stage esophagectomy, especially in low-volume centers, to decrease these high-risk cardio-pulmonary complications.

The impact of robotic cholecystectomy on private practice in a community teaching hospital.

BACKGROUND: Recently, through aggressive marketing, robotic cholecystectomy has been gaining popularity. The purpose of this study was to evaluate the impact of this technology on private practice and hospital cost and volume. METHODS: From November 2012 to April 2014, all elective cholecystectomies were evaluated for procedure type, operative time (OR), insurance type and payment, hospital length of stay, and volume. Data were analyzed using the Chi-square test, Student t test and the Mann-Whitney U test. RESULTS: Of 338 patients, 246 had complete financial records. Of these patients, 84.1% (207) patients were female with mean age of 45.4 ± 17.1 years. Patients were divided into 2 groups; group 1: 220(89.4%) patients had laparoscopic cholecystectomy (LC) and group 2: 26(10.6%) patients had robotic cholecystectomy (RC). The mean direct cost was higher in the robotic group $2,704.08 ± 308.40 vs $1,712.51 ± 379.50; P < .0001. The median gross margin; however, was not statically different (RC: $1,593.00 (Interquartile range $3,936) vs LC: $1,726.00 (Interquartile range $1,480); P = .85). Both case time and OR were higher in the robotic group; case time (RC: 121 ± 15.4 vs LC: 98.4 ± 27.5 minutes, P < .0001); OR (RC: 86.6 ± 14.3 vs LC: 63.9 ± 25.9 minutes, P < .0001). There was no appreciable change over time in either surgeon or hospital volume. CONCLUSIONS: There was a statistically significant increase in direct cost in RC vs. LC but not in margin. There was no impact in private practice on the number of cases being done robotically, nor there was an increase in hospital volume. This analysis did not include the purchase cost or maintenance of the robot.

Advanced glycation end product in familial amyloidotic polyneuropathy (FAP).

OBJECTIVES: Advanced glycation end products (AGE) are present in amyloid deposits in beta2-microglobulin amyloidosis, and it has been postulated that glycation of beta2-microglobulin may be involved in fibril formation. The aim of this paper was to ascertain whether AGE occur in amyloid deposits in familial amyloidotic polyneuropathy (FAP). SETTING: Department of Medicine, Umeå University Hospital and First Department of Internal Medicine, Kumamoto University School of Medicine. DESIGN: The presence of AGE was sought immunohistochemically and biochemically in amyloid-rich tissues from patients with FAP. SUBJECTS: Biopsy specimens from nine patients and 10 controls were used for the immunohistochemical analysis. For amyloid preparation, vitreous samples from three FAP patients were used. RESULTS: Immunohistochemical studies using a polyclonal anti-AGE antibody revealed positive immunoreactivity in intestinal materials, but the pattern of reactivity was unevenly distributed; it was often present in the border of amyloid deposits, or surrounding them. Non-amyloid associated immunoreactivity was also observed in a few regions of the specimens, although the AGE-positive structures were situated in areas containing amyloid deposits. Western blotting of purified amyloid from the vitreous body of FAP patients revealed a significant association of AGE with amyloid fibrils. CONCLUSION: The immunoreactivity for the AGE antibody suggests that AGE may be involved in fibril formation in FAP.

Oxidative stress is found in amyloid deposits in systemic amyloidosis.

Oxidative stress in human amyloid deposits from patients with systemic amyloidosis, such as familial amyloidotic polyneuropathy (FAP), primary amyloidosis, and secondary amyloidosis, was examined immunohistochemically by means of an affinity purified anti-hydroxynonenal (HNE) antibody to HNE, a marker of lipid peroxidation. Furthermore, the levels of thiobarbituric acid reactive substances (TBARS), which is another marker of lipid peroxidation, and the levels of protein carbonyl, a maker of protein modifications by free radicals, were determined in FAP patients and healthy controls. Affinity purified anti-HNE antibody reacted with amyloid deposits in all types of amyloid tissues examined. Significantly higher levels of TBARS and protein carbonyl were found in amyloid rich tissues of FAP patients compared with those in the control subjects. These results verify that lipid peroxidation via free radical injury occurs in amyloid deposits in systemic amyloidosis. The protein modifying properties of free radicals increase the likelihood that they play an important role in the amyloid formation process.