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2.
Environ Res ; 216(Pt 4): 114669, 2023 01 01.
Article in English | MEDLINE | ID: mdl-36404520

ABSTRACT

This article has been withdrawn: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been withdrawn at the request of the authors, editor and publisher. The publisher regrets that an error occurred which led to the premature publication of this paper. The publisher apologizes to the readers for this unfortunate erro

4.
Chemosphere ; 308(Pt 1): 136278, 2022 Dec.
Article in English | MEDLINE | ID: mdl-36057349

ABSTRACT

Groundwater is usually utilized as a drinking water asset everywhere. Therefore, groundwater defilement by poisonous radioactive metals such as uranium (VI) is a major concern due to the increase in nuclear power plants as well as their by-products which are released into the watercourses. Waste Uranium (VI) can be regarded as a by-product of the enrichment method used to produce atomic energy, and the hazard associated with this is due to the uranium radioactivity causing toxicity. To manage these confronts, there are so many techniques that have been introduced but among those adsorptions is recognized as a straightforward, successful, and monetary innovation, which has gotten major interest nowadays, despite specific drawbacks regarding operational as well as functional applications. This review summarizes the various adsorbents such as Bio-adsorbent/green materials, metal oxide-based adsorbent, polymer based adsorbent, graphene oxide based adsorbent, and magnetic nanomaterials and discuss their synthesis methods. Furthermore, this paper emphasis on adsorption process by various adsorbents or modified forms under different physicochemical conditions. In addition to this adsorption mechanism of uranium (VI) onto different adsorbent is studied in this article. Finally, from the literature reviewed conclusion have been drawn and also proposed few future research suggestions.


Subject(s)
Drinking Water , Uranium , Adsorption , Hydrogen-Ion Concentration , Kinetics , Polymers , Uranium/analysis , Wastewater
5.
Int J Biol Macromol ; 165(Pt A): 333-345, 2020 Dec 15.
Article in English | MEDLINE | ID: mdl-32980413

ABSTRACT

The current study aims to check various behavioural, developmental, cytotoxic, and genotoxic effects of Fe3O4-GG nanocomposite (GGNCs) on Drosophila melanogaster. Fe3O4 nanoparticles were prepared by the chemical co-precipitation method and cross-linked with guargum nanoparticles to prepare the nanocomposites. The nanocomposites were characterized by using transmission electron microscopy (TEM), X-ray diffraction (XRD), and FTIR techniques. To investigate the biomolecular interaction, GGNCs was further tagged with Fluorescein isothiocyanate. Various concentrations of nanocomposites were mixed with the food and flies were allowed to complete the life cycle. The life cycle of the flies was studied as a function of various concentrations of GGNCs. The 1st instar larvae after hatching from the egg start eating the food mixed with GGNCs. The 3rd instar larvae were investigated for various behavioural and morphological abnormalities within the gut. The 3rd instar larva has defective crawling speed, crawling path, and more number of micronuclei within the gut. Similarly, in adult flies thermal sensitivity, climbing behaviour was found to be altered. In adult flies, a significant reduction in body weight was found which is further correlated with variation of protein, carbohydrate, triglyceride, and antioxidant enzymes. Altogether, the current study suggests GGNCs as a non-genotoxic nanoparticle for various biological applications.


Subject(s)
Coated Materials, Biocompatible , DNA Damage , Ferrosoferric Oxide , Galactans , Mannans , Plant Gums , Animals , Coated Materials, Biocompatible/chemistry , Coated Materials, Biocompatible/pharmacology , Drosophila melanogaster , Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/pharmacology , Galactans/chemistry , Galactans/pharmacology , Larva/growth & development , Mannans/chemistry , Mannans/pharmacology , Plant Gums/chemistry , Plant Gums/pharmacology
6.
J Photochem Photobiol B ; 198: 111563, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31369959

ABSTRACT

Bone Morphogenetic Protein 2 (BMP-2) is an osteoinductive protein which has been overexpressed, refolded (Refolding is often the bottle-neck step in producing recombinant proteins from inclusion bodies of Escherichia coli, especially for dimer proteins) and purified by using Heparin affinity chromatography. Refolding of BMP-2 was based on gradient dialysis in presence of lower urea concentration. The main objective of the present work is to unravel the impact of the extracellular matrix components on BMP-2 conformation and stability. We tried to elucidate the interaction of the bone matrix minerals in the form of nanoparticles with the bone protein. We chose hydroxyapatite nanoparticles (HAp NPs) which is the most abundant bone mineral and the other being a trace mineral in our bones i.e., zinc oxide nanoparticles (ZnO NPs) as the potential nanoparticles for this study. The isolated protein is found to be a ß- sheet type with melting temperature being approximately 70.66 °C. Upon interaction with HAp NPs and ZnO NPs, the absorbance and the fluorescence intensity indicates the interaction with the protein as there was an upsurge in both the cases. Circular Dichroism (CD) spectroscopy revealed that ZnO NPs are having more dominant secondary structure and thermal stabilizing effect as compared to HAp NPs.


Subject(s)
Bone Morphogenetic Protein 2/chemistry , Durapatite/chemistry , Nanoparticles/chemistry , Bone Morphogenetic Protein 2/genetics , Bone Morphogenetic Protein 2/metabolism , Circular Dichroism , Humans , Protein Stability , Protein Structure, Secondary , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/isolation & purification , Spectrometry, Fluorescence , Temperature , Zinc Oxide/chemistry
7.
J Basic Microbiol ; 58(7): 643, 2018 Jul.
Article in English | MEDLINE | ID: mdl-26617413

ABSTRACT

The above article from the Journal of Basic Microbiology, published online on 25 August 2015 in Wiley Online Library as Early View (http://onlinelibrary.wiley.com/doi/10.1002/jobm.201500135/pdf), has been retracted by agreement between Naveen Kumar Navani and Ranjana Pathania, the Editor-in-Chief and Wiley-VCH GmbH & Co. KGaA. The retraction has been agreed because the article has been submitted and approved for publication by Jitendra Kumar Sahoo without consent in any form by the named co-authors Naveen Kumar Navani and Ranjana Pathania.

8.
Waste Manag ; 33(7): 1595-601, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23663960

ABSTRACT

Bacterial diversity of full scale rotary drum composter from biodegradable organic waste samples were analyzed through two different approaches, i.e., Culture dependent and independent techniques. Culture-dependent enumerations for indigenous population of bacterial isolates mainly total heterotrophic bacteria (Bacillus species, Pseudomonas species and Enterobacter species), Fecal Coliforms, Fecal Streptococci, Escherichia coli, Salmonella species and Shigella species showed reduction during the composting period. On the other hand, Culture-independent method using PCR amplification of specific 16S rRNA sequences identified the presence of Acinetobacter species, Actinobacteria species, Bacillus species, Clostridium species, Hydrogenophaga species, Butyrivibrio species, Pedobacter species, Empedobactor species and Flavobacterium species by sequences clustering in the phylogenetic tree. Furthermore, correlating physico-chemical analysis of samples with bacterial diversity revealed the bacterial communities have undergone changes, possibly linked to the variations in temperature and availability of new metabolic substrates while decomposing organics at different stages of composting.


Subject(s)
Bacteria/genetics , Bacteria/isolation & purification , Phylogeny , Refuse Disposal/instrumentation , Refuse Disposal/methods , Bacteria/classification , Biodiversity , Equipment Design , Manure , Microbial Consortia/genetics , Molecular Sequence Data , Polymerase Chain Reaction , RNA, Ribosomal, 16S , Soil , Soil Microbiology , Temperature , Vegetables
9.
J Basic Microbiol ; 52(1): 5-15, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21953506

ABSTRACT

This study investigates the prevailing microbial communities during the composting of vegetable waste, cattle manure and saw dust, in a household (250 l) batch scale Rotary Drum composter and Windrow Pile. Physico-chemical parameters were analyzed to study the organic matter transformations. Total organic matter reduced from 63.8% to 36.2% in rotary drum and 39.6% in windrow pile composting. The C/N ratio decreased from 26.52 to 8.89 and 14.33 in rotary drum and windrow pile composting. The indigenous population of total heterotrophic bacteria decreased in rotary drum and windrow pile composting after 20 days. However, total fungal load initially increased within initial 4 days, then subsequently reduced in final composts. The average number of fecal coliforms and fecal Streptococci showed decrement with time, in both composting systems. Escherichia coli and Salmonella species number deduced during the study. Composting cycle started with Gram positive rods but ended up with the dominance of Gram negative bacilli shaped bacteria. Transformation of organic compounds during the biodegradation of organic waste, difference in the utilization of nutrients (organic matter) by the different group of microbes and high temperature could be cited as a possible reason of the above changes. Scanning electron microscopy has been used to obtain the surface structures of the cultured mycoflora. Results of the study revealed that higher diversity of microbes prevailed in rotary drum as compared to windrow pile, yielding more stable and pathogenic free compost in lesser period of composting.


Subject(s)
Refuse Disposal/methods , Soil Microbiology , Biodiversity , Escherichia coli/isolation & purification , Fungi/isolation & purification , Microscopy, Electron, Scanning , Salmonella/isolation & purification , Soil/chemistry
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