ABSTRACT
Non-small-cell-lung-cancer (NSCLC) constitutes about 75-80% of lung cancers. The challenge to tackle cancers is in early diagnosis and arriving at safer therapeutic options. In vitro studies using cancer cell lines continue to contribute significantly in understanding cancers. Cell culture methods have evolved and the recent developments in 3 dimensional (3D) cell cultures are inducing greater resemblance of the in vitro cultured cells with in vivo conditions. In this study, we established 3D aggregates of H460 cell line on agarose hydrogels and studied the protein expression differences among cells grown as monolayers (2D) and the progressively developing 3D aggregates from days 2 to 10. Analysis included matching of those proteins expressed by the developing aggregates and the available literature on progressing tumors in vivo. J. Cell. Biochem. 118: 1648-1652, 2017. © 2016 Wiley Periodicals, Inc.
Subject(s)
Biomarkers, Tumor/metabolism , Carcinoma, Non-Small-Cell Lung/metabolism , Cell Culture Techniques , Cell Line, Tumor , Electrophoresis, Polyacrylamide Gel , Gene Expression Regulation, Neoplastic/genetics , Gene Expression Regulation, Neoplastic/physiology , Humans , Lung Neoplasms/metabolismABSTRACT
Various types of cancer continue to be subjects of intense research because of the impact of these diseases and their socioeconomic implications. Also, the complexity involved in the pathogenesis, nature of the triggers, and the progression of cancers is intriguing. An important aspect of cancers is the genetics involved, and studies involving cancer genes contributed immensely in not only understanding cancers better, but also for obtaining useful markers and therapy targets. We review the salient features, functions, and changes in gene expression for 103 carcinoma genes, 20 sarcoma genes, and 36 lymphoma genes. Apart from the three major levels of cancer type, we discuss the implications of altered gene expression at the tissue level as well. The possible uses of these gene functions and expression changes for diagnostic, prognostic, and therapeutic applications are presented. Also, the 159 genes are assessed for their involvement in more than a single cancer and tissue type. Only the p53 gene is commonly implicated in carcinomas, sarcoma and lymphomas. The CHEK2 and ERBB2 (HER2) genes are commonly found to be associated with carcinomas and sarcomas, whereas the MDM2, MSH2, and MSH6 genes are commonly implicated among carcinomas and lymphomas.
