Assessment of sperm quality, DNA integrity and cryopreservation protocols in men diagnosed with testicular and systemic malignancies.

Men diagnosed with malignancy are often referred for semen banking to preserve their fertility prior to cancer treatment. The chances of cancer patients for achieving future fecundity will be determined by the sperm quality including the integrity of the genomic material in the frozen samples. The objectives of this study were to compare the sperm quality and DNA integrity in men diagnosed with testicular and systemic malignancies before receiving treatment and to identify the optimum cryopreservation protocol for their samples including a remote semen collection option. In comparison with fertile donors, patients with testicular malignancies had significantly lower sperm concentration, while both testicular and systemic malignancy patients had significantly lower sperm motility and cryosurvival rates. In addition, the SCSA defined DNA fragmentation index was significantly higher in patients with testicular and systemic malignancies compared with fertile donors. It was noted that the extent of deterioration in sperm quality and DNA integrity seen in cancer patients did not reach the previously defined statistical threshold for impaired fertility. Freezing spermatozoa with the seminal plasma offers the highest protection against cryo-injury. Nevertheless, remote semen collection can still be used as it yields adequate results.

Relationship between sperm apoptosis signalling and oocyte penetration capacity.

Human sperm have been documented to display apoptosis-like features such as externalization of phosphatidylserine (EPS), disruption of the transmembrane mitochondrial potential (MMP) and activation of caspases. Our aim was to evaluate possible association between activation of the apoptosis cascade in human sperm and its oocyte penetration capacity using the zona free hamster oocyte penetration assay (SPA). Semen specimens from 76 unselected donors were subjected to double density gradient centrifugation followed by incubation under capacitating conditions for 3 h and SPA. Apoptosis signalling was monitored by assessment of EPS, disruption of MMP and activation of caspase-3 by flow cytometry. Semen samples with subnormal SPA values (<20% penetrated oocytes) contained significantly higher amounts of spermatozoa with EPS, disrupted MMP and activated caspase-3 compared with those samples with normal SPA values (>20% penetrated oocytes, p < 0.01). All three apoptosis markers showed a significantly negative correlation with the percentage of penetrated oocytes (p < 0.01). Apoptosis-related signalling appears to have a negative association with sperm-oocyte penetration. The exclusion of sperm presenting with those apoptosis-related features during assisted reproduction may improve success rates of procedures such as intrauterine insemination and in vitro fertilization.

Between myth and madness: the premigration dream of leaving among young Somali refugees.

Many young Somali refugees experience long premigration waits and a poorly delimited transition period in a succession of countries before reaching their final destination. During this difficult passage, a myth dealing with departure and exodus is collectively constructed, and it serves as a dynamic, mobilizing dream that orients individual strategies. This substitution of "dream travel" for real travel during the transition period, especially if it is prolonged, may cause Somali youths to lose contact with reality and eventually to slide into madness. The authors' approach is based on three assumptions: (a) that pastoralism predisposes the Somali to value travel as a way of maturing, (b) that age-based peer groups create special migratory dynamics, and (c) that an ethic of solidarity involves many people in the adventure of a migrant youth. When trapped in an indefinite transition period, young men share khat-chewing sessions during which they relate success stories and dreams of leaving. Many grow frustrated with the delay, and if their departure plans fall through, the "dream trip" often becomes "dream madness." Actual cases illustrate how some young Somali get lost in their dreams. A young Somali's vulnerability is heightened when he extricates himself from the system of reciprocal obligations or when the liminal stage ends with the mourning of the impossible dream. In the universe of madness visited by some young Somali migrants, the boundaries between the real and the imaginary are poorly marked. The paper is based on fieldwork carried out in the Horn of Africa and in Canada, interviews with Somali immigrants and members of the community, and clinical psychiatric data collected in Montreal.