PCMT1 knockdown attenuates malignant properties by globally regulating transcriptome profiles in triple-negative breast cancer cells.

Background: As the most frequently diagnosed cancer in women, Breast cancer has high mortality and metastasis rate, especially triple-negative breast cancer (TNBC). As an oncogene, protein-L-isoaspartate (D-aspartate) O-methyltransferase (PCMT1) is a prognostic biomarker in breast cancer and is highly expressed, while its underlying functions remain unknown. Methods: In this study, we silenced PCTM1 in TNBC MDA-MB-231 cells by short hairpin RNA (shPCMT1) to investigate its cellular functions using cell proliferation, apoptosis, migration, and invasion experiments. Following this, the transcriptome sequencing (RNA-seq) experiment was conducted to explore the molecular targets of PCMT1, including differentially expressed genes (DEGs) and regulated alternative splicing events (RASEs). Results: The results showed that shPCMT1 inhibited the proliferation, migration, and invasion of MDA-MB-231 cells. We obtained 1,084 DEGs and 2,287 RASEs between shPCMT1 and negative control (NC) groups through RNA-seq. The DEGs were significantly enriched in immune or inflammation response and cell adhesion-associated pathways, pathways associated with PCMT1 cellular function in cell migration. The RASE genes were enriched in cell cycle-associated pathways and were associated with the altered cell proliferation rate. We finally validated the changed expression and splicing levels of DEGs and RASEs. We found that 34 RNA binding protein (RBP) genes were dysregulated by shPCMT1, including NQO1, S100A4, EEF1A2, and RBMS2. The dysregulated RBP genes could partially explain how PCMT1 regulates the global transcriptome profiles. Conclusion: In conclusion, our study identified the molecular targets of PCMT1 in the TNBC cell line, expands our understanding of the regulatory mechanisms of PCMT1 in cancer progression, and provides novel insights into the progression of TNBC. The identified molecular targets are potential therapeutic targets for future TNBC treatment.

Expression of Ang-2 and HSP90α in serum of patients with non-small cell lung cancer and its correlation with TNM staging / 国际检验医学杂志

Objective The expression of Ang-2 and HSP90α in serum of patients with non-small cell lung cancer and their correlation with TNM staging were analyzed.Methods From January 2015 to June 2016,40 patients with elderly non-small cell lung cancer and 40 healthy persons were selected as the study subjects.The serum levels of Ang-2 and HSP90 alpha in the subjects were examined and their correlation with the TNM stage of the patients was analyzed.Results Observation of Ang-2 and HSP90α levels in the serum of the patients group were significantly higher than the control group,and the difference was statistical significance(P<0.05);Serum Ang-2 and HSP90α levels TNM Ⅰ-Ⅱ patients were significantly lower than that of stage Ⅲ and Ⅳ,the difference was statistical significance(P<0.05);Serum Ang-2 and HSP90α levels in TNM Ⅲ patients was significantly lower than those in stage Ⅲ and Ⅳ,the difference was statistical significance(P<0.05);TNM staging was positively correlated with the levels of HSP90α in serum,and statistical significance(r=0.425,P<0.05),TNM stage had a remarkably positive correlation with the level of Ang in serum(r=0.525,P<0.05).Conclusion The levels of Ang-2 and HSP90α were significantly higher in the serum of elderly patients with non-small cell lung cancer,and the expression level was positively correlated with TNM stage.

Clinical and prognostic analysis of 70 cases of gastric stromal tumors / 国际外科学杂志

Objective To discuss factors related to clinical characteristics,biological behavior,and prognosis in gastric stromal tumors (GST).Methods We retrospectively analyzed clinical materials and records of 70 GST patients diagnosed after surgery and pathological examinations,all patients were contacted by telephone or mail for follow-up.Results (1) All GST patients had similar distribution of gender,and had age range of 50 to 69 years with mean age of 59.1 years.Clinical symptoms were non-specific,and main clinical manifestations included abdominal discomfort (42.9％),followed by abdominal pain (37.1％),and upper digestive tract hemorrhage (7.1％).(2) Sex,age,presenting symptoms,and original location of tumors were not significantly related to prognosis (P ＞ 0.05).However,size of the tumor,mitosis count,and the malignancy potential grading were significantly associated with patient prognosis.Among them,size of the tumor (maximum tumor diameter ＞ 5 cm vs ＜ 2 cm,HR =21.3,95％ CI:5.9-77.0;maximum tumor diameter 2-5 cm vs ＜ 2 cm,HR =2.3,95％ CI:1.2-7.8),and mitosis count (mitosis count ＞ 10/50 HPF vs mitosis count ≤5/50 HPF,HR =22.5,95％ CI:8.6-58.6;6-10/50 HPF vs ≤5/50 HPF,HR =11.1,95％ CI:9.9-12.3) were independently associated with GST prognosis.Conclusions Tumor size,mitosis count,and malignancy potential grading were associated with prognosis of GST.Among them,tumor size and mitosis count were independent predictors of prognosis.When the tumor size is larger,and mitotic count and the malignant potential grade is higher,prognosis of GST patients were worse.

Prevalence of and risk factors for gallstones in Uighur and Han Chinese.

AIM: To perform a single-centre survey of the prevalence of and possible risk factors for gallstones in Uighur and Han Chinese. METHODS: Complete medical data for 9455 patients were collected from the medical centre of our hospital, and the overall prevalence of gallstones as well as the prevalence in different ethnic groups was studied. The risk factors for gallstones in different ethnic groups were identified in a univariate analysis, and variables with statistical significance were analysed by unconditional multiple logistic regression, to primarily explore the similarities and differences in gallstone risk factors between different ethnic groups. RESULTS: The prevalence of gallstones was significantly higher in the Uighur population than in the Han population (22.87% vs 11.64%, P < 0.05). Further analysis of risk factors for gallstones based on the different ethnic areas revealed that age was a risk factor for gallstones in both groups; triglycerides, body-mass index (BMI) and high-density lipoprotein were risk factors for gallstones in the Han population, while total cholesterol (TC), gender and fatty liver were risk factors in the Uighur population. The Uighur patients were older than the Han patients, and had higher BMI, TC, low-density lipoprotein, female rate and fatty liver rate, while the incidence of hypertension was lower than that in the Han patients. CONCLUSION: The prevalence of and risk factors for gallstones differ between the Uighur and Han populations.