ABSTRACT
Whole-genome sequencing of non-H(2)S-producing Salmonella enterica serovar Typhimurium and S. enterica serovar Infantis isolates from poultry meat revealed a nonsense mutation in the phsA thiosulfate reductase gene and carriage of a CMY-2 ß-lactamase. The lack of production of H(2)S might lead to the incorrect identification of S. enterica isolates carrying antimicrobial resistance genes.
Subject(s)
Poultry Diseases/microbiology , Salmonella Infections, Animal/microbiology , Salmonella enterica/genetics , Animals , Genome, Bacterial , Hydrogen Sulfide/metabolism , Japan , Salmonella enterica/enzymology , Salmonella enterica/isolation & purification , Salmonella enterica/metabolism , Sequence Analysis, DNA , Sulfurtransferases/genetics , beta-Lactamases/geneticsABSTRACT
The ingestion of undercooked meat from wild animals can be a source of Toxoplasma gondii infection in humans and other animals. In this study, we determined the seroprevalence of T. gondii infection in 175 wild boars (Sus scrofa leucomystax) and 107 wild sika deer (Cervus nippon) hunted in 2004-2007 in Gunma Prefecture, Japan, by using a commercial latex agglutination test (LAT). Antibodies (LAT, 1:64 or higher) to T. gondii were found in 6.3% of wild boars and 1.9% of sika deer. This is the first record of T. gondii infection in wild deer in Japan, and deer and wild boar meat should be cooked well before human consumption.
Subject(s)
Antibodies, Protozoan/blood , Deer/parasitology , Sus scrofa/parasitology , Swine Diseases/epidemiology , Toxoplasma/immunology , Toxoplasmosis, Animal/epidemiology , Animals , Confidence Intervals , Female , Humans , Japan/epidemiology , Latex Fixation Tests/methods , Latex Fixation Tests/veterinary , Male , Meat/parasitology , Seroepidemiologic Studies , Swine , Swine Diseases/parasitologyABSTRACT
The prevalence of hepatitis E virus (HEV) infection in wild boars and pigs in Gunma Prefecture, Japan, was serologically and genetically examined. The positive detection rates of anti-HEV IgG and HEV RNA in the wild boars were 4.5% (4/89) and 1.1% (1/89), whereas those in the pigs were 74.6% (126/169) and 1.8% (3/169), respectively. The positive rates of anti-HEV IgG and HEV RNA on the 17 pig farms in the present study ranged from 20% to 100%, respectively. One male wild boar approximately 5 years of age was positive for HEV RNA but was negative for anti-HEV IgG. Three pigs from 2 farms were positive for HEV RNA; 2 of these pigs were negative for HEV IgG, and the other was positive. A phylogenetic analysis revealed that all of the HEV ORF1 genes detected in the present study belonged to genotype III. In Gunma Prefecture, HEV is highly prevalent and widespread, and uncooked wild boar and pig meat may have the potential to transmit HEV to humans.
