Determination of volatile aroma composition of some hazelnut varieties grown in Akçakoca conditions.

Türkiye is leading country for hazelnut production and hazelnut orchards are widely established in Black Sea belt. Akçakoca district belongs to Duzce province is accepted as one of the most important production areas. In this research, volatile aroma components in nuts (kernels) of Çakildak, Kara, Sari (Mincane), Tombul and Yomra hazelnut varieties grown in Akçakoca district were determined by SPME/GC-MS. The analysis revealed the presence a total of 55 different volatile aroma components. These analyzes identified the presence of 39 descriptive volatile aroma compounds with significant differences between varieties. Çakildak variety differed from the other varieties in terms of Isopentyl alcohol and Ethyl acetate components. At the same time, some volatile aroma compounds are commonly detected among the all varieties. This study has shown that the climate and soil conditions of Akçakoca cause the emergence of unique taste profiles through the effect of hazelnut varieties on volatile aroma components. The findings emphasize that regional diversity and local ecosystem factors play a critical role in determining flavor in hazelnut kernels. This study also emphasizes that variety selection is a critical factor in ensuring sustainability in hazelnut cultivation and that determining volatile aroma components is an important indicator in this selection.

Pomegranate Peel Phytochemistry, Pharmacological Properties, Methods of Extraction, and Its Application: A Comprehensive Review.

Pomegranate peel, derived from the processing of Punica granatum L. (pomegranate), has traditionally been considered agricultural waste. However, recent studies have revealed its potential as a rich source of bioactive compounds with diverse pharmacological effects. Pomegranate peel is a rich reservoir of antioxidants, polyphenols, dietary fiber, and vitamins, which contribute to its remarkable bioactivity. Studies have demonstrated the anti-inflammatory, cardioprotective, wound healing, anticancer, and antimicrobial properties of pomegranate peel owing to the presence of phytochemicals, such as gallic acid, ellagic acid, and punicalagin. The extraction of bioactive compounds from pomegranate peel requires a careful selection of techniques to maximize the yield and quality. Green extraction methods, including pressurized liquid extraction (PLE), ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE), and enzyme-assisted extraction (EAE), offer efficient and sustainable alternatives to traditional methods. Furthermore, pomegranate peel has been utilized in the food industry, where it can significantly enhance the nutritional value, organoleptic characteristics, and shelf life of food products. Pomegranate peel has the potential to be used to develop innovative functional foods, nutraceuticals, and other value-added products, providing new opportunities for the pharmaceutical, cosmetic, and food industries.

γ-tocopherol methyltransferase gene sequencing and SNP discovery associated with olive oil quality.

γ-tocophérol methyltransferase (GTMT), a key enzyme in the tocopherols biosynthesis pathway, is involved in the conversion of δ- and l'γ-tocophérol to ß- et l'α-tocophérol, respectively. In fact, it plays an important role in the α-tocopherol composition and the quality of olive oil. A total of 14 olive tree cultivars (Olea Europaea L.) were chosen and used in this study. They were sampled from different regions of Tunisia. Four cultivars from four Mediterranean regions (Greece, Algeria, Morocco, and Spain) were included for comparison. For each variety, DNA was extracted from young leaves. The Vte4 gene was PCR amplified from the 14 olive varieties and verified by electrophoresis on a 2% agarose gel for each variety. DNA sequencing of the olive cultivars revealed several single-nucleotide polymorphisms (SNPs). Statistical and bioinformatics analysis  draw attention to some associations between some of the SNPs, tocopherols contents and oleic acid content. In fact, two significant associations are obtained between SUBS24 and both Total-Tocopherols and Beta-Tocopherol. Moreover, dendrograms reveals that there is a correlation between genetic variability and chemical characteristics which make the Vte4 gene more interesting in terms of tocopherols levels.

Application of Plant-Based Edible Coatings and Extracts Influences the Postharvest Quality and Shelf Life Potential of "Surahi" Guava Fruits.

Guava fruits have a short shelf life due to climacteric nature. The current work was conducted to extend the shelf life of guavas with garlic extract (GRE), ginger extract (GNE), gum arabic (GA), and Aloe vera (AV) gel coatings. After coating, fruits of guava were stored at 25 ± 3 °C and RH 85 ± 2% for 15 days. Results showed that guavas treated with plant-based edible coatings and extracts had lower weight loss than that of the control. GRE-treated guavas had the maximum shelf life in contrast to all other treatments including the control. GNE-treated guavas showed the lowest nonreducing sugar content, whereas they had higher antioxidant activity, vitamin C content, and total phenolics compared with all other coating treatments. After the control, antioxidant capacity was the highest in GNE- and GRE-treated fruits. On the other hand, GA-treated guavas had reduced total soluble solids and juice pH (more acidic) and exhibited higher total flavonoids compared with the control, while both GA- and GNE-treated guavas had the highest flavonoid content. GRE-treated fruits exhibited the highest total sugar content and taste and aroma scores. In conclusion, GRE treatment was more effective in conserving the quality and extending the shelf life of guava fruits.

Pistachio, Pomegranate and Olive Byproducts Added to Sheep Rations Change the Biofunctional Properties of Milk through the Milk Amino Acid Profile.

This study was carried out to determine the effects of adding pistachio shell (PIS), pomegranate hull (POM), and olive pulp (OP) to the diet on milk amino acid and fatty acid parameters in Awassi sheep. In the study, 40 head of Awassi sheep, which gave birth at least twice, were used as animal material. Sheep were fed a control diet without added byproducts (CON), rations containing PIS, POM, and OP. Milk amino acid profile was determined by liquid chromatography-tandem mass spectrometry, milk fatty acid gas chromatography-flame ionization detection device. There was a dramatic reduction in alanine, citrulline, glutamine, glutamic acid, glycine, leucine, ornithine and alphaaminoadipic acid in the research groups. In the PIS group, argininosuccinic acid, gammaminobutyric acid, beta-alanine and sarcosine; In the POM group, asparagine, gammaminobutyric acid, beta-alanine, and taurine; In the OP group, a significant positive increase was found in terms of alanine, histidine, gammaminobutyric acid, and taurine amino acids. The applications in the study did not have a statistically significant effect on the ratio of short, medium and long chain fatty acids in milk (p>0.05). In the presented study, it was determined that PIS, POM, and OP, which were added to the sheep rations at a rate of 5%, caused significant changes in the milk amino acid profiles. In this change in milk amino acid profiles, the benefit-harm relationship should be considered.

Genetic Diversity Among Historical Olive (Olea europaea L.) Genotypes from Southern Anatolia Based on SSR Markers.

Olive (Olea europaea) is an ancient and important crop in both olive oil production and table use. It is important to identify the genetic diversity of olive genetic resources for cultivar development and evaluation of olive germplasm. In the study, 14 microsatellite markers (UDO4, UDO8, UDO9, UDO11, UDO12, UDO22, UDO24, UDO26, UDO28, DCA9, DCA11, DCA13, DCA15, and DCA18) were used to assess the genetic variation on 76 olive (Olea europaea L.) genotypes from Mardin province together with 6 well-known Turkish and 4 well-known foreign reference cultivars. All microsatellite markers showed polymorphism and the number of alleles varied between 9 and 22, with an average of 14.57. The most informative loci were DCA 11 (22 alleles) and DCA 9 (21 alleles). Dendrogram based on genetic distances was constructed for the 86 olive genotypes/cultivars, which revealed the existence of different clusters. The high genetic similarity was evident between Bakirkire2 and Zinnar5 (0.74) genotypes, while the most genetically divergent genotypes were Gürmese5 and Yedikardesler2 (0.19). It was concluded that there was abundant SSR polymorphism in olive germplasm in southern Anatolia in Turkey and could be important for future breeding activities.

Genetic Relationships Among Olive (Olea europaea L.) Cultivars Native to Turkey.

Olive is a widely cultivated, mainly in the Mediterranean region, and economically important fruit species used as both olive oil and table olive consumption. In Turkey, more than 50 olive cultivars have been authorized for commercial plantations, representing the developmental base for the olive industry. The aim of the present study was to identify genetic relationships among the most widely grown 27 olive cultivars in Turkey, using microsatellite or simple sequence repeat markers. Nine well-known foreign olive cultivars from different countries are also included in the study to compare the Turkish cultivars. To determine genetic relationship and diversity, 10 SSR loci (DCA3, DCA9, DCA15, DCA18, UDO4, UDO9, UDO11, UDO12, UDO24, UDO28) were used. Jaccard's similarity coefficient and the UPGMA method for cluster analysis were performed using the software NTSYSpc. The results showed that the number of alleles per locus ranging from 4 (UDO4, UDO9, UDO11, UDO12, DCA15) to 12 (DCA9) presenting high polymorphism. There were no identical cultivars. High similarity was shown by cultivars Maviand Adana topagi (0.754). The most genetically divergent cultivars, Domat-Meski (0.240) and Domat-NizipYaglik (0.245), were also identified.