ABSTRACT
A new acylated neohesperoside derivative, 1-octyl-4'-isovaleroyl-neohesperoside (1), was isolated from Geranium purpureum Vill. (Geraniaceae) together with the known compounds quercetin-3-rutinoside and gallic acid. The identification of the isolated compounds was carried out by spectroscopic analysis including 1D- and 2D- NMR (1H, 13C, COSY, HSQC and HMBC) spectroscopy and ESI-TOF-MS.
Subject(s)
Disaccharides/chemistry , Geranium/chemistry , Molecular Structure , Spectrum AnalysisABSTRACT
An investigation was made of the effects on endogenous antioxidant enzyme activities and H2O2-induced lipid peroxidation inhibition in human red blood cells of the crude MeOH extract and its EtOAc, n-BuOH, and H2O sub-extracts obtained from aerial parts of Geranium psilostemon Ledeb., as well as compounds isolated from the most active EtOAc extract. Gallic acid (1), methyl gallate (2), pusilagin (3), 1,3,6-tri-O-galloyl-beta-glucopyranoside (4), 1,2,3,4,6-penta-O-galloyl-beta-glucopyranoside (5), kaempferol (6), quercetin (7), kaempferol 7-O-alpha-rhamnopyranoside (8), and quercetin 7-O-alpha-rhamnopyranoside (9) were isolated from the aerial parts of the title plant, and their structures identified from spectroscopic (UV, 1D- and 2D- NMR) and spectrometric (TOF-MS) data. All extracts and isolated compounds inhibited H2O2-induced lipid peroxidation and also enhanced the activity of superoxide dismutase (SOD) and catalase (CAT).
Subject(s)
Antioxidants/chemistry , Antioxidants/metabolism , Geranium/chemistry , Geranium/metabolism , Erythrocytes/drug effects , Erythrocytes/metabolism , Humans , Hydrogen Peroxide , Lipid Peroxidation , Plant Components, Aerial/chemistryABSTRACT
In this study, it was shown that abietic acid, an abietane diterpenoid, inhibited soybean 5-lipoxygenase (linoleate: oxygen oxidoreductase, EC 1.13.11.12) and an IC(50) of 29.5 +/- 1.29 microM was determined. Since the lipoxygenase pathway leads to the biosynthesis of leukotrienes this result supports the view that abietic acid may be used in the treatment of allergic reactions.
