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1.
Proc Natl Acad Sci U S A ; 112(35): 10914-9, 2015 Sep 01.
Article in English | MEDLINE | ID: mdl-26283381

ABSTRACT

DNA mismatch repair (MMR) identifies and corrects errors made during replication. In all organisms except those expressing MutH, interactions between a DNA mismatch, MutS, MutL, and the replication processivity factor (ß-clamp or PCNA) activate the latent MutL endonuclease to nick the error-containing daughter strand. This nick provides an entry point for downstream repair proteins. Despite the well-established significance of strand-specific nicking in MMR, the mechanism(s) by which MutS and MutL assemble on mismatch DNA to allow the subsequent activation of MutL's endonuclease activity by ß-clamp/PCNA remains elusive. In both prokaryotes and eukaryotes, MutS homologs undergo conformational changes to a mobile clamp state that can move away from the mismatch. However, the function of this MutS mobile clamp is unknown. Furthermore, whether the interaction with MutL leads to a mobile MutS-MutL complex or a mismatch-localized complex is hotly debated. We used single molecule FRET to determine that Thermus aquaticus MutL traps MutS at a DNA mismatch after recognition but before its conversion to a sliding clamp. Rather than a clamp, a conformationally dynamic protein assembly typically containing more MutL than MutS is formed at the mismatch. This complex provides a local marker where interaction with ß-clamp/PCNA could distinguish parent/daughter strand identity. Our finding that MutL fundamentally changes MutS actions following mismatch detection reframes current thinking on MMR signaling processes critical for genomic stability.


Subject(s)
Bacterial Proteins/genetics , Base Pair Mismatch , Thermus/genetics , Genes, Bacterial
2.
J Biol Chem ; 287(13): 9777-9791, 2012 Mar 23.
Article in English | MEDLINE | ID: mdl-22277660

ABSTRACT

The heterodimeric human MSH2-MSH6 protein initiates DNA mismatch repair (MMR) by recognizing mismatched bases that result from replication errors. Msh2(G674A) or Msh6(T1217D) mice that have mutations in or near the ATP binding site of MSH2 or ATP hydrolysis catalytic site of MSH6 develop cancer and have a reduced lifespan due to loss of the MMR pathway (Lin, D. P., Wang, Y., Scherer, S. J., Clark, A. B., Yang, K., Avdievich, E., Jin, B., Werling, U., Parris, T., Kurihara, N., Umar, A., Kucherlapati, R., Lipkin, M., Kunkel, T. A., and Edelmann, W. (2004) Cancer Res. 64, 517-522; Yang, G., Scherer, S. J., Shell, S. S., Yang, K., Kim, M., Lipkin, M., Kucherlapati, R., Kolodner, R. D., and Edelmann, W. (2004) Cancer Cell 6, 139-150). Mouse embryonic fibroblasts from these mice retain an apoptotic response to DNA damage. Mutant human MutSα proteins MSH2(G674A)-MSH6(wt) and MSH2(wt)-MSH6(T1219D) are profiled in a variety of functional assays and as expected fail to support MMR in vitro, although they retain mismatch recognition activity. Kinetic analyses of DNA binding and ATPase activities and examination of the excision step of MMR reveal that the two mutants differ in their underlying molecular defects. MSH2(wt)-MSH6(T1219D) fails to couple nucleotide binding and mismatch recognition, whereas MSH2(G674A)-MSH6(wt) has a partial defect in nucleotide binding. Nevertheless, both mutant proteins remain bound to the mismatch and fail to promote efficient excision thereby inhibiting MMR in vitro in a dominant manner. Implications of these findings for MMR and DNA damage signaling by MMR proteins are discussed.


Subject(s)
Amino Acid Substitution , DNA Mismatch Repair/physiology , DNA-Binding Proteins/chemistry , DNA/chemistry , MutS Homolog 2 Protein/chemistry , Mutation, Missense , Animals , Cells, Cultured , DNA/genetics , DNA/metabolism , DNA Damage , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Embryo, Mammalian , Fibroblasts/metabolism , Humans , Kinetics , Mice , Mice, Mutant Strains , MutS Homolog 2 Protein/genetics , MutS Homolog 2 Protein/metabolism , Protein Binding
3.
J Mol Biol ; 416(2): 163-75, 2012 Feb 17.
Article in English | MEDLINE | ID: mdl-22197374

ABSTRACT

Replication factor C (RFC) is a five-subunit complex that loads proliferating cell nuclear antigen (PCNA) clamps onto primer-template DNA (ptDNA) during replication. RFC subunits belong to the AAA(+) superfamily, and their ATPase activity drives interactions between the clamp loader, the clamp, and the ptDNA, leading to topologically linked PCNA·ptDNA. We report the kinetics of transient events in Saccharomyces cerevisiae RFC-catalyzed PCNA loading, including ATP-induced RFC activation, PCNA opening, ptDNA binding, ATP hydrolysis, PCNA closing, and PCNA·ptDNA release. This detailed perspective enables assessment of individual RFC-A, RFC-B, RFC-C, RFC-D, and RFC-E subunit functions in the reaction mechanism. Functions have been ascribed to RFC subunits previously based on a steady-state analysis of 'arginine-finger' ATPase mutants; however, pre-steady-state analysis provides a different view. The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP binding to this subunit initiates RFC activation, and the clamp loader adopts a spiral conformation that stabilizes PCNA in a corresponding open spiral. The importance of RFC subunit response to ATP binding decreases as RFC-C>RFC-D>RFC-B, with RFC-A being unnecessary. RFC-C-dependent activation of RFC also enables ptDNA binding, leading to the formation of the RFC·ATP·PCNA(open)·ptDNA complex. Subsequent ATP hydrolysis leads to complex dissociation, with RFC-D activity contributing the most to rapid ptDNA release. The pivotal role of the RFC-B/C/D subunit ATPase core in clamp loading is consistent with the similar central location of all three ATPase active subunits of the Escherichia coli clamp loader.


Subject(s)
DNA Replication , Proliferating Cell Nuclear Antigen/chemistry , Replication Protein C/chemistry , Saccharomyces cerevisiae Proteins/chemistry , Saccharomyces cerevisiae/metabolism , Adenosine Triphosphatases/chemistry , Adenosine Triphosphatases/metabolism , Adenosine Triphosphate/metabolism , DNA, Fungal/chemistry , DNA, Fungal/metabolism , Hydrolysis , Kinetics , Models, Molecular , Mutation , Proliferating Cell Nuclear Antigen/genetics , Proliferating Cell Nuclear Antigen/metabolism , Protein Conformation , Protein Subunits/chemistry , Protein Subunits/genetics , Protein Subunits/metabolism , Replication Protein C/genetics , Replication Protein C/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism
4.
J Mol Biol ; 416(2): 176-91, 2012 Feb 17.
Article in English | MEDLINE | ID: mdl-22197378

ABSTRACT

The multi-subunit replication factor C (RFC) complex loads circular proliferating cell nuclear antigen (PCNA) clamps onto DNA where they serve as mobile tethers for polymerases and coordinate the functions of many other DNA metabolic proteins. The clamp loading reaction is complex, involving multiple components (RFC, PCNA, DNA, and ATP) and events (minimally: PCNA opening/closing, DNA binding/release, and ATP binding/hydrolysis) that yield a topologically linked clamp·DNA product in less than a second. Here, we report pre-steady-state measurements of several steps in the reaction catalyzed by Saccharomyces cerevisiae RFC and present a comprehensive kinetic model based on global analysis of the data. Highlights of the reaction mechanism are that ATP binding to RFC initiates slow activation of the clamp loader, enabling it to open PCNA (at ~2 s(-1)) and bind primer-template DNA (ptDNA). Rapid binding of ptDNA leads to formation of the RFC·ATP·PCNA(open)·ptDNA complex, which catalyzes a burst of ATP hydrolysis. Another slow step in the reaction follows ATP hydrolysis and is associated with PCNA closure around ptDNA (8 s(-1)). Dissociation of PCNA·ptDNA from RFC leads to catalytic turnover. We propose that these early and late rate-determining events are intramolecular conformational changes in RFC and PCNA that control clamp opening and closure, and that ATP binding and hydrolysis switch RFC between conformations with high and low affinities, respectively, for open PCNA and ptDNA, and thus bookend the clamp loading reaction.


Subject(s)
Adenosine Triphosphate/metabolism , Proliferating Cell Nuclear Antigen/metabolism , Replication Protein C/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Adenosine Triphosphate/chemistry , Binding Sites , Catalysis , DNA Primers/chemistry , DNA Primers/metabolism , DNA, Fungal/chemistry , DNA, Fungal/metabolism , Hydrolysis , Models, Molecular , Proliferating Cell Nuclear Antigen/chemistry , Protein Conformation , Replication Protein C/chemistry , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/chemistry
5.
J Biol Chem ; 286(46): 40287-95, 2011 Nov 18.
Article in English | MEDLINE | ID: mdl-21937421

ABSTRACT

The mechanics of hMSH2-hMSH6 ATP binding and hydrolysis are critical to several proposed mechanisms for mismatch repair (MMR), which in turn rely on the detailed coordination of ATP processing between the individual hMSH2 and hMSH6 subunits. Here we show that hMSH2-hMSH6 is strictly controlled by hMSH2 and magnesium in a complex with ADP (hMSH2(magnesium-ADP)-hMSH6). Destabilization of magnesium results in ADP release from hMSH2 that allows high affinity ATP binding by hMSH6, which then enhances ATP binding by hMSH2. Both subunits must be ATP-bound to efficiently form a stable hMSH2-hMSH6 hydrolysis-independent sliding clamp required for MMR. In the presence of magnesium, the ATP-bound sliding clamps remain on the DNA for ∼8 min. These results suggest a precise stepwise kinetic mechanism for hMSH2-hMSH6 functions that appears to mimic G protein switches, severely constrains models for MMR, and may partially explain the MSH2 allele frequency in Lynch syndrome or hereditary nonpolyposis colorectal cancer.


Subject(s)
Adenosine Triphosphate/chemistry , DNA-Binding Proteins/chemistry , Multienzyme Complexes/chemistry , MutS Homolog 2 Protein/chemistry , Adenosine Diphosphate/chemistry , Adenosine Diphosphate/genetics , Adenosine Diphosphate/metabolism , Adenosine Triphosphate/genetics , Adenosine Triphosphate/metabolism , Colorectal Neoplasms, Hereditary Nonpolyposis/enzymology , Colorectal Neoplasms, Hereditary Nonpolyposis/genetics , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Humans , Hydrolysis , Kinetics , Multienzyme Complexes/genetics , Multienzyme Complexes/metabolism , MutS Homolog 2 Protein/genetics , MutS Homolog 2 Protein/metabolism
6.
J Mol Biol ; 388(3): 431-42, 2009 May 08.
Article in English | MEDLINE | ID: mdl-19285992

ABSTRACT

Circular clamps tether polymerases to DNA, serving as essential processivity factors in genome replication, and function in other critical cellular processes as well. Clamp loaders catalyze clamp assembly onto DNA, and the question of how these proteins construct a topological link between a clamp and DNA, especially the mechanism by which ATP is utilized for the task, remains open. Here we describe pre-steady-state analysis of ATP hydrolysis, proliferating cell nuclear antigen (PCNA) clamp opening, and DNA binding by Saccharomyces cerevisiae replication factor C (RFC), and present the first kinetic model of a eukaryotic clamp-loading reaction validated by global data analysis. ATP binding to multiple RFC subunits initiates a slow conformational change in the clamp loader, enabling it to bind and open PCNA and to bind DNA as well. PCNA opening locks RFC into an active state, and the resulting RFC.ATP.PCNA((open)) intermediate is ready for the entry of DNA into the clamp. DNA binding commits RFC to ATP hydrolysis, which is followed by PCNA closure and PCNA.DNA release. This model enables quantitative understanding of the multistep mechanism of a eukaryotic clamp loader and furthermore facilitates comparative analysis of loaders from diverse organisms.


Subject(s)
Adenosine Triphosphate/metabolism , Proliferating Cell Nuclear Antigen/metabolism , Replication Protein C/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Allosteric Regulation , DNA, Fungal/metabolism , Kinetics , Models, Biological , Protein Binding , Protein Conformation
7.
Methods Cell Biol ; 91: 161-71, 2009.
Article in English | MEDLINE | ID: mdl-20409787

ABSTRACT

Axonemal dyneins are multi-megadalton complexes which consist of heavy chains (HCs), intermediate chains (ICs), and light chains (LCs). The configuration and interactions among the many components within the dynein complex are not fully understood. For initial investigation of protein-protein interactions, chemical crosslinking can be easily applied to either flagellar axonemes or purified dyneins. Careful selection of crosslinker enables one to target protein-protein interactions that are constitutive and also to identify alterations in the configuration of the complex. For example, when performed in the presence of nucleotide or ligands such as Ca(2+), it is possible to trap transient interactions under specific physiological condition. Here I first describe the preparation of a crosslinked sample and its analysis by electrophoresis and immunoblotting using antibodies raised against a target and candidate interaction proteins. Next, when an interaction partner cannot be simply identified by immunoblotting, a crosslinked product may be isolated by immunoprecipitation, and its composition determined by mass spectrometry. These general approaches have great potential to define protein-protein interactions within any macromolecular complex of interest.


Subject(s)
Cross-Linking Reagents/chemistry , Dyneins/chemistry , Animals , Axoneme/chemistry , Chlamydomonas/cytology , Dyneins/ultrastructure , Humans , Immunoblotting/methods
8.
Eukaryot Cell ; 7(7): 1136-45, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18487347

ABSTRACT

The outer dynein arm of Chlamydomonas flagella contains three heavy chains (alpha, beta, and gamma), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest. Here we report the isolation of a novel mutant, oda2-t, whose gamma heavy chain is truncated at about 30% of the sequence. While the previously isolated gamma chain mutant oda2 lacks the entire outer arm, oda2-t retains outer arms that contain alpha and beta heavy chains, suggesting that the N-terminal sequence (corresponding to the tail region) is necessary and sufficient for stable outer-arm assembly. Thin-section electron microscopy and image analysis localize the gamma heavy chain to a basal region of the outer-arm image in the axonemal cross section. The motility of oda2-t is lower than that of the wild type and oda11 (lacking the alpha heavy chain) but higher than that of oda2 and oda4-s7 (lacking the motor domain of the beta heavy chain). Thus, the outer-arm dynein lacking the gamma heavy-chain motor domain is partially functional. The availability of mutants lacking individual heavy chains should greatly facilitate studies on the structure and function of the outer-arm dynein.


Subject(s)
Chlamydomonas/enzymology , Dyneins/metabolism , Flagella/enzymology , Mutation , Protozoan Proteins/metabolism , Adenosine Triphosphatases/chemistry , Adenosine Triphosphatases/genetics , Adenosine Triphosphatases/metabolism , Animals , Blotting, Western , Chlamydomonas/chemistry , Chlamydomonas/genetics , Chlamydomonas/physiology , Dyneins/chemistry , Dyneins/genetics , Dyneins/ultrastructure , Flagella/chemistry , Flagella/genetics , Flagella/physiology , Protein Subunits/chemistry , Protein Subunits/genetics , Protein Subunits/metabolism , Protozoan Proteins/chemistry , Protozoan Proteins/genetics , Protozoan Proteins/ultrastructure
9.
Nucleic Acids Res ; 36(10): 3274-86, 2008 Jun.
Article in English | MEDLINE | ID: mdl-18424802

ABSTRACT

The Escherichia coli clamp loader, gamma complex (gamma(3)deltadelta'lambdapsi), catalyzes ATP-driven assembly of beta clamps onto primer-template DNA (p/tDNA), enabling processive replication. The mechanism by which gamma complex targets p/tDNA for clamp assembly is not resolved. According to previous studies, charged/polar amino acids inside the clamp loader chamber interact with the double-stranded (ds) portion of p/tDNA. We find that dsDNA, not ssDNA, can trigger a burst of ATP hydrolysis by gamma complex and clamp assembly, but only at far higher concentrations than p/tDNA. Thus, contact between gamma complex and dsDNA is necessary and sufficient, but not optimal, for the reaction, and additional contacts with p/tDNA likely facilitate its selection as the optimal substrate for clamp assembly. We investigated whether a conserved sequence-HRVW(279)QNRR--in delta subunit contributes to such interactions, since Tryptophan-279 specifically cross-links to the primer-template junction. Mutation of delta-W279 weakens gamma complex binding to p/tDNA, hampering its ability to load clamps and promote proccessive DNA replication, and additional mutations in the sequence (delta-R277, delta-R283) worsen the interaction. These data reveal a novel location in the C-terminal domain of the E. coli clamp loader that contributes to DNA binding and helps define p/tDNA as the preferred substrate for the reaction.


Subject(s)
DNA Polymerase III/chemistry , DNA Replication , DNA/metabolism , Escherichia coli Proteins/chemistry , Adenosine Triphosphatases/metabolism , Adenosine Triphosphate/metabolism , Amino Acid Motifs , Conserved Sequence , DNA Polymerase III/genetics , DNA Polymerase III/metabolism , DNA Primers/metabolism , Escherichia coli Proteins/genetics , Escherichia coli Proteins/metabolism , Mutagenesis, Site-Directed , Protein Binding , Templates, Genetic , Tryptophan/chemistry , Tryptophan/genetics
10.
Methods Mol Biol ; 392: 71-83, 2007.
Article in English | MEDLINE | ID: mdl-17951711

ABSTRACT

Dyneins are highly complex molecular motors containing multiple components that contribute motor, regulatory and cargo-binding activities. Within cilia/flagella, these enzymes comprise the inner and outer arms associated with the doublet microtubules. In this chapter, we describe how to purify the outer dynein arm from flagella of the unicellular green alga Chlamydomonas, which is one of the best characterized members of this motor class. We also detail the methods that we use to identify interactions involving dynein components by chemical cross-linking and a recently developed technique to assess the in vivo redox state of thioredoxin-like proteins that are associated with axonemal dyneins from a wide range of organisms. Finally, we describe how to purify highly specific antibodies from serum by blot purification using recombinant proteins. Although designed for analysis of Chlamydomonas flagellar dyneins, these approaches should be readily adaptable to the study of other systems.


Subject(s)
Biochemistry/methods , Dyneins/chemistry , Gene Expression Regulation , Oxidation-Reduction , Animals , Axons/metabolism , Centrifugation, Density Gradient , Chlamydomonas/metabolism , Cilia/metabolism , Cross-Linking Reagents/pharmacology , Flagella/metabolism , Protein Binding , Protein Structure, Tertiary , Proteomics/methods , Thioredoxins/chemistry
11.
Mol Biol Cell ; 18(9): 3620-34, 2007 Sep.
Article in English | MEDLINE | ID: mdl-17634291

ABSTRACT

We have previously shown that Ca(2+) directly activates ATP-sensitive microtubule binding by a Chlamydomonas outer arm dynein subparticle containing the beta and gamma heavy chains (HCs). The gamma HC-associated LC4 light chain is a member of the calmodulin family and binds 1-2 Ca(2+) with K(Ca) = 3 x 10(-5) M in vitro, suggesting it may act as a Ca(2+) sensor for outer arm dynein. Here we investigate interactions between the LC4 light chain and gamma HC. Two IQ consensus motifs for binding calmodulin-like proteins are located within the stem domain of the gamma heavy chain. In vitro experiments indicate that LC4 undergoes a Ca(2+)-dependent interaction with the IQ motif domain while remaining tethered to the HC. LC4 also moves into close proximity of the intermediate chain IC1 in the presence of Ca(2+). The sedimentation profile of the gamma HC subunit changed subtly upon Ca(2+) addition, suggesting that the entire complex had become more compact, and electron microscopy of the isolated gamma subunit revealed a distinct alteration in conformation of the N-terminal stem in response to Ca(2+) addition. We propose that Ca(2+)-dependent conformational change of LC4 has a direct effect on the stem domain of the gamma HC, which eventually leads to alterations in mechanochemical interactions between microtubules and the motor domain(s) of the outer dynein arm.


Subject(s)
Calcium/pharmacology , Chlamydomonas/metabolism , Dyneins/chemistry , Amino Acid Motifs , Amino Acid Sequence , Animals , Antibodies , Cross-Linking Reagents/pharmacology , Cytoplasm/drug effects , Cytoplasm/metabolism , Dyneins/ultrastructure , Molecular Sequence Data , Mutant Proteins/metabolism , Protein Binding/drug effects , Protein Structure, Tertiary/drug effects , Protein Subunits/chemistry , Protein Subunits/metabolism
12.
Mol Biol Cell ; 16(12): 5661-74, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16195342

ABSTRACT

Tctex1 and Tctex2 were originally described as potential distorters/sterility factors in the non-Mendelian transmission of t-haplotypes in mice. These proteins have since been identified as subunits of cytoplasmic and/or axonemal dyneins. Within the Chlamydomonas flagellum, Tctex1 is a subunit of inner arm I1. We have now identified a second Tctex1-related protein (here termed LC9) in Chlamydomonas. LC9 copurifies with outer arm dynein in sucrose density gradients and is missing only in those strains completely lacking this motor. Zero-length cross-linking of purified outer arm dynein indicates that LC9 interacts directly with both the IC1 and IC2 intermediate chains. Immunoblot analysis revealed that LC2, LC6, and LC9 are missing in an IC2 mutant strain (oda6-r88) that can assemble outer arms but exhibits significantly reduced flagellar beat frequency. This defect is unlikely to be due to lack of LC6, because an LC6 null mutant (oda13) exhibits only a minor swimming abnormality. Using an LC2 null mutant (oda12-1), we find that although some outer arm dynein components assemble in the absence of LC2, they are nonfunctional. In contrast, dyneins from oda6-r88, which also lack LC2, retain some activity. Furthermore, we observed a synthetic assembly defect in an oda6-r88 oda12-1 double mutant. These data suggest that LC2, LC6, and LC9 have different roles in outer arm assembly and are required for wild-type motor function in the Chlamydomonas flagellum.


Subject(s)
Carrier Proteins/physiology , Chlamydomonas reinhardtii/physiology , Drosophila Proteins/physiology , Dyneins/physiology , Amino Acid Sequence , Animals , Base Sequence , Binding Sites , Carrier Proteins/chemistry , Carrier Proteins/genetics , Chlamydomonas reinhardtii/drug effects , Drosophila Proteins/chemistry , Drosophila Proteins/genetics , Ethyldimethylaminopropyl Carbodiimide/pharmacology , Humans , Mice , Molecular Sequence Data , Sequence Alignment , Sequence Homology, Amino Acid
13.
Mol Biol Cell ; 15(10): 4633-46, 2004 Oct.
Article in English | MEDLINE | ID: mdl-15304520

ABSTRACT

Members of the LC7/Roadblock family of light chains (LCs) have been found in both cytoplasmic and axonemal dyneins. LC7a was originally identified within Chlamydomonas outer arm dynein and associates with this motor's cargo-binding region. We describe here a novel member of this protein family, termed LC7b that is also present in the Chlamydomonas flagellum. Levels of LC7b are reduced approximately 20% in axonemes isolated from strains lacking inner arm I1 and are approximately 80% lower in the absence of the outer arms. When both dyneins are missing, LC7b levels are diminished to <10%. In oda9 axonemal extracts that completely lack outer arms, LC7b copurifies with inner arm I1, whereas in ida1 extracts that are devoid of I1 inner arms it associates with outer arm dynein. We also have observed that some LC7a is present in both isolated axonemes and purified 18S dynein from oda1, suggesting that it is also a component of both the outer arm and inner arm I1. Intriguingly, in axonemal extracts from the LC7a null mutant, oda15, which assembles approximately 30% of its outer arms, LC7b fails to copurify with either dynein, suggesting that it interacts with LC7a. Furthermore, both the outer arm gamma heavy chain and DC2 from the outer arm docking complex completely dissociate after salt extraction from oda15 axonemes. EDC cross-linking of purified dynein revealed that LC7b interacts with LC3, an outer dynein arm thioredoxin; DC2, an outer arm docking complex component; and also with the phosphoprotein IC138 from inner arm I1. These data suggest that LC7a stabilizes both the outer arms and inner arm I1 and that both LC7a and LC7b are involved in multiple intradynein interactions within both dyneins.


Subject(s)
Carrier Proteins/metabolism , Chlamydomonas reinhardtii/metabolism , Dyneins/metabolism , Molecular Motor Proteins/metabolism , Protozoan Proteins/metabolism , Amino Acid Sequence , Animals , Carrier Proteins/classification , Carrier Proteins/genetics , Chlamydomonas reinhardtii/cytology , Dyneins/chemistry , Dyneins/genetics , Flagella/metabolism , Flagella/ultrastructure , Humans , Molecular Motor Proteins/genetics , Molecular Sequence Data , Phylogeny , Plant Proteins , Protein Isoforms/genetics , Protein Isoforms/metabolism , Protozoan Proteins/classification , Protozoan Proteins/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism
14.
J Struct Biol ; 146(1-2): 58-71, 2004.
Article in English | MEDLINE | ID: mdl-15037237

ABSTRACT

Dyneins are highly complex molecular motors that transport their attached cargo towards the minus end of microtubules. These enzymes are required for many essential motile activities within the cytoplasm and also power eukaryotic cilia and flagella. Each dynein contains one or more heavy chain motor units that consist of an N-terminal stem domain that is involved in cargo attachment, and six AAA+ domains (AAA1-6) plus a C-terminal globular segment that are arranged in a heptameric ring. At least one AAA+ domain (AAA1) is capable of ATP binding and hydrolysis, and the available data suggest that one or more additional domains also may bind nucleotide. The ATP-sensitive microtubule binding site is located at the tip of a 10nm coiled coil stalk that emanates from between AAA4 and AAA5. The function of this motor both in the cytoplasm and the flagellum must be tightly regulated in order to result in useful work. Consequently, dyneins also contain a series of additional components that serve to define the cargo-binding properties of the enzyme and which act as sensors to transmit regulatory inputs to the motor units. Here we describe the two basic dynein designs and detail the various regulatory systems that impinge on this motor within the eukaryotic flagellum.


Subject(s)
Dyneins/chemistry , Binding Sites , Dyneins/metabolism , Dyneins/physiology , Microtubule Proteins , Molecular Motor Proteins , Protein Conformation
15.
J Biol Chem ; 278(44): 43571-9, 2003 Oct 31.
Article in English | MEDLINE | ID: mdl-12923201

ABSTRACT

The Chlamydomonas outer dynein arm contains three distinct heavy chains (alpha, beta, and gamma) that exhibit different motor properties. The LC4 protein, which binds 1-2 Ca2+ with KCa = 3 x 10-5 m, is associated with the gamma heavy chain and has been proposed to act as a sensor to regulate dynein motor function in response to alterations in intraflagellar Ca2+ levels. Here we genetically dissect the outer arm to yield subparticles containing different motor unit combinations and assess the microtubule-binding properties of these complexes both prior to and following preincubation with tubulin and ATP, which was used to inhibit ATP-insensitive (structural) microtubule binding. We observed that the alpha heavy chain exhibits a dominant Ca2+-independent ATP-sensitive MT binding activity in vitro that is inhibited by attachment of tubulin to the structural microtubule-binding domain. Furthermore, we show that ATP-sensitive microtubule binding by a dynein subparticle containing only the beta and gamma heavy chains does not occur at Ca2+ concentrations below pCa 6 but is maximally activated above pCa 5. This activity was not observed in mutant dyneins containing small deletions in the microtubule-binding region of the beta heavy chain or in dyneins that lack both the alpha heavy chain and the motor domain of the beta heavy chain. These findings strongly suggest that Ca2+ binding directly to a component of the dynein complex regulates ATP-sensitive interactions between the beta heavy chain and microtubules and lead to a model for how individual motor units are controlled within the outer dynein arm.


Subject(s)
Adenosine Triphosphate/metabolism , Calcium/metabolism , Chlamydomonas/metabolism , Chlamydomonas/physiology , Dyneins/chemistry , Animals , Brain/metabolism , Cattle , Dyneins/metabolism , Electrophoresis, Polyacrylamide Gel , Kinetics , Microtubules/metabolism , Models, Biological , Protein Binding
16.
Cell Motil Cytoskeleton ; 52(3): 131-43, 2002 Jul.
Article in English | MEDLINE | ID: mdl-12112141

ABSTRACT

The outer dynein arm from Chlamydomonas flagella contains two redox-active thioredoxin-related light chains associated with the alpha and beta heavy chains; these proteins belong to a distinct subgroup within the thioredoxin family. This observation suggested that some aspect of dynein activity might be modulated through redox poise. To test this, we have examined the effect of sulfhydryl oxidation on the ATPase activity of isolated dynein and axonemes from wildtype and mutant strains lacking various heavy chain combinations. The outer, but not inner, dynein arm ATPase was stimulated significantly following treatment with low concentrations of dithionitrobenzoic acid; this effect was readily reversible by dithiol, and to a lesser extent, monothiol reductants. Mutational and biochemical dissection of the outer arm revealed that ATPase activation in response to DTNB was an exclusive property of the gamma heavy chain, and that enzymatic enhancement was modulated by the presence of other dynein components. Furthermore, we demonstrate that the LC5 thioredoxin-like light chain binds to the N-terminal stem domain of the alpha heavy chain and that the beta heavy chain-associated LC3 protein also interacts with the gamma heavy chain. These data suggest the possibility of a dynein-associated redox cascade and further support the idea that the gamma heavy chain plays a key regulatory role within the outer arm.


Subject(s)
Chlamydomonas/enzymology , Dyneins/metabolism , Amino Acid Sequence , Animals , Dithionitrobenzoic Acid/metabolism , Flagella/metabolism , Models, Molecular , Molecular Sequence Data , Oxidation-Reduction , Protein Structure, Tertiary , Sequence Alignment , Structure-Activity Relationship
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