Subject(s)
Heat-Shock Proteins/biosynthesis , Intestinal Mucosa/transplantation , Jejunum/transplantation , Organ Preservation , Transplantation, Isogeneic/physiology , Adenosine , Allopurinol , Animals , Arsenites/pharmacology , Cold Temperature , Gene Expression Regulation/drug effects , Glutathione , Graft Survival , Heat-Shock Proteins/genetics , Insulin , Intestinal Mucosa/cytology , Intestinal Mucosa/physiology , Jejunum/cytology , Jejunum/physiology , Male , Organ Preservation Solutions , Raffinose , Rats , Rats, Inbred BN , Sodium Compounds/pharmacology , Transplantation, Isogeneic/pathologySubject(s)
Graft Survival/physiology , Intestinal Mucosa/transplantation , Intestine, Small/transplantation , Organ Preservation Solutions , Organ Preservation/methods , Transplantation, Isogeneic/physiology , Animals , Glutamine , Intestinal Absorption , Intestinal Mucosa/cytology , Intestinal Mucosa/physiology , Intestine, Small/cytology , Intestine, Small/physiology , Male , Rats , Rats, Inbred Lew , Sodium Chloride , Time Factors , Transplantation, Isogeneic/pathologySubject(s)
Glutamine/pharmacology , Intestinal Mucosa , Jejunum , Organ Preservation Solutions , Organ Preservation/methods , Adenosine , Allopurinol , Animals , Glucose/metabolism , Glutathione , Insulin , Intestinal Absorption/drug effects , Intestinal Mucosa/physiology , Jejunum/physiology , Raffinose , Rats , Rats, Inbred Lew , Sodium ChlorideSubject(s)
Glutamine/pharmacology , Intestinal Mucosa/pathology , Jejunum/pathology , Organ Preservation Solutions , Organ Preservation , Adenosine , Allopurinol , Amino Acids/pharmacology , Animals , Cold Temperature , Glutathione , Insulin , Intestinal Mucosa/drug effects , Jejunum/drug effects , Raffinose , Rats , Rats, Inbred Lew , Time FactorsABSTRACT
This study investigated the inhibitory effect of human macrophage colony stimulating factor (hM-CSF) on experimental liver metastases. Murine colon cancer cell lines C-26 and C-36 were each injected into the spleen of BALB/c mice and the number of subsequent liver metastases was counted. An optimal dose (3,000U/body/day) of hM-CSF to inhibit liver metastases was identified. Additionally, hM-CSF (3,000U/body/day) administration for 7 days before and after the injection of the same cancer cell lines was investigated. The mean number of liver metastases decreased significantly in the hM-CSF group compared with the saline group. These results suggest potential therapeutic benefit for the use of hM-CSF in the adjuvant treatment of liver metastases.
Subject(s)
Liver Neoplasms, Experimental/prevention & control , Liver Neoplasms, Experimental/secondary , Macrophage Colony-Stimulating Factor/therapeutic use , Animals , Colonic Neoplasms/pathology , Dose-Response Relationship, Drug , Granulocyte Colony-Stimulating Factor/therapeutic use , Male , Mice , Mice, Inbred BALB CABSTRACT
Serum CA 50 was determined by a time resolved fluorometric immunoassay (TR-FIA) with CANAG CA-50 DELFIA kit. Evaluation of the assay system gave satisfactory results in its sensitivity, accuracy, reproducibility, dynamic range and easy handling. No prozone phenomenon was observed up to 347,000 U/ml. From a histogram of 134 normal sera, the cut off point was determined at 34 U/ml. CA 50 in 202 patients' sera was determined with this assay. Nineteen of 20 patients pancreatic cancer, 6 of 21 gastric cancer, 14 of 25 hepatoma gave positive values. In comparison with CA 19-9, higher values and higher rates of positive CA 50 were observed in benign and malignant liver diseases, suggesting its non-cancerous origin in the liver. A high correlation was observed between the level of CA 50 and CA 19-9 of 157 patients' sera. Serum CA 50 was completely correlated with CA 19-9 in the clinical course of patients with pancreatic cancer, but not in patients with hepatoma. Thus we conclude that the CANAG CA-50 DELFIA System is useful for the diagnosis and monitoring cancer patients but must be used with care because of its elevation in benign liver diseases.
Subject(s)
Antigens, Tumor-Associated, Carbohydrate/analysis , Fluorescent Antibody Technique , Humans , Neoplasms/diagnosis , Neoplasms/immunology , Predictive Value of Tests , Reference Values , Reproducibility of ResultsABSTRACT
A 22-year old man was admitted because of an abnormal shadow on his chest X-ray film. Radiographic findings revealed that the tumor was located in the right middle mediastinum. At operation a smooth round tumor was found arising from the right phrenic nerve. The tumor was removed with phrenic nerve. Pathohistological examination revealed this tumor was benign schwannoma. Intrathoracic schwannoma usually arise from intercostal and sympathetic nerve and that arising from phrenic nerve is very rare. We removed the tumor by means of cutting his right phrenic nerve because of complete resection. Postoperatively he did well without any respiratory distress. And his respiratory function studies recovered normal 9 months after the operation. We think that schwannoma arising from phrenic nerve should be removed completely and in such a case cutting of the one side phrenic nerve is at ease if the patient have normal respiratory function.
Subject(s)
Neurilemmoma , Peripheral Nervous System Neoplasms , Phrenic Nerve , Adult , Humans , Male , Neurilemmoma/diagnostic imaging , Neurilemmoma/pathology , Neurilemmoma/surgery , Peripheral Nervous System Neoplasms/diagnostic imaging , Peripheral Nervous System Neoplasms/pathology , Peripheral Nervous System Neoplasms/surgery , Tomography, X-Ray ComputedSubject(s)
Phosphopyruvate Hydratase/blood , Radioimmunoassay/methods , Reagent Kits, Diagnostic/standards , Adolescent , Adult , Child , Child, Preschool , Diagnosis, Differential , Evaluation Studies as Topic , Eye Neoplasms/diagnosis , Humans , Infant , Neoplasm Recurrence, Local/diagnosis , Neuroblastoma/diagnosis , Retinoblastoma/diagnosisABSTRACT
Three new antigens reacting with monoclonal antibodies; CA 19/9, CA 125 and DuPan 2, were evaluated for their clinical significance in the diagnosis of pancreatic carcinoma. CA 19/9 is confirmed to be the best marker in its specificity (97%) and sensitivity (85%). Even in patients with small carcinoma, 5 of 6 T 1 cases showed positive CA 19/9 values. Transient elevations were noted in obstructive jaundice either with malignant tumors or benign but chronic inflammatory process. CA 125 is elevated in 85% of patients with pancreatic carcinoma. It is considered to be a marker indicating serosal, namely peritoneal or pleural, invasion not only in malignant but also in benign diseases. A sandwich solid phase radioimmunometric assay for DuPan 2 was established and elevated antigenic levels were detected in 80% of patients with advanced pancreatic carcinoma.
