Subject(s)
Embryonic Development/drug effects , beta-Endorphin/pharmacology , Animals , Blastocyst/cytology , Blastocyst/drug effects , Blastomeres/cytology , Blastomeres/drug effects , Embryonic Development/physiology , Female , Mice , Mice, Inbred Strains , Morula/cytology , Morula/drug effects , beta-Endorphin/physiologyABSTRACT
The localization of enhanced green fluorescent protein (EGFP) was studied in preimplantation embryos obtained from reciprocal mating of hemizygous C57Bl/6-Tgn (ACTbEGFP)1Osb/J mice with C57Bl/6 mice. Specific fluorescence of EGFP was observed in all oocytes and embryos obtained from transgenic females during all preimplantation stages and in embryos inheriting the EGFP gene from transgenic males starting from the 8 blastomere stage during the compactization period. EGFP mRNA or EGFP synthesized during oogenesis can be retained in embryos during the entire preimplantation period, while expression of EGFP gene transferred from the father coincides with the onset of compactization. The possibility of using these embryos in experimental mammalian embryology is discussed.
Subject(s)
Blastocyst/physiology , Green Fluorescent Proteins/metabolism , Animals , Blastocyst/cytology , Female , Green Fluorescent Proteins/genetics , Male , Mice , Mice, Inbred C57BL , Oocytes/cytology , Oocytes/metabolismABSTRACT
By means of thiocholine method, ACHE activity has been revealed in cells of ganglia, ventral nerves, nervous plexuses of the pharynx and subepidermal layer of four planarian species. The products of ACHE activity appeared in the form of dark granules localized over cellular bodies and processes. Interspecific differences in the number and topography of ACHE-reactive cells were demonstrated.
