ABSTRACT
The preclinical analysis of the effectiveness of AZT and new compounds (sulphatized derivate of chitosan (Sch) and adamantileted Sch, contents 10 and 18% of adamantine) prepared on the basis of chitosan (Ch), carried out with the use of the experimental clinical test system (the in vitro model of peripheral blood mononuclears of a concrete patient) developed in laboratory, is presented. Both combinations in concentration 0.01 mkg/ml are decreased HIV antigens till 6.4 and 8.6% accordingly, while whilst initial Sch hare caused less effect in 4-5 fold.
Subject(s)
Anti-HIV Agents/therapeutic use , HIV Infections/drug therapy , HIV Protease Inhibitors/therapeutic use , HIV-1 , Anti-HIV Agents/antagonists & inhibitors , Drug Resistance, Microbial , Drug Therapy, Combination , Humans , Zidovudine/antagonists & inhibitors , Zidovudine/therapeutic useABSTRACT
A changeable membrane filter preventing the friction between moving parts and the membrane during tight screwing of a culture flask is proposed.
Subject(s)
Cell Culture Techniques/instrumentation , Membranes, Artificial , Animals , Cells, Cultured , Equipment Design , Filtration/instrumentation , HumansABSTRACT
The principal component method is verified for analysis of categorized biological characteristics of HIV-1 variants. Thirty wild isolates of HIV-1 are studied. The method notably facilitated comparative analysis of a large scope of experimental data. Using this method, it is possible to detect a relationship between the biological characteristics and results of other taxonomic schemes, to distinguish the factors restricting the biological signs, to single out the most informative signs for describing a given set of isolates, and to define the criteria for comparing the properties of two sets of virus isolates and selecting the isolates notably differing from each other for more thorough phenotyping.
Subject(s)
HIV-1/physiology , Cell Line , HIV-1/classification , HIV-1/genetics , Humans , Microbiological Techniques , PhenotypeSubject(s)
Epitopes/immunology , HIV Antibodies/immunology , HIV Envelope Protein gp120/chemistry , Peptide Fragments/chemistry , Amino Acid Sequence , DNA, Viral , Epitopes/chemistry , HIV Envelope Protein gp120/genetics , HIV Infections/blood , HIV-1/chemistry , HIV-1/genetics , HIV-1/immunology , Humans , Immunoenzyme Techniques , Models, Chemical , Molecular Sequence Data , Peptide Fragments/genetics , Proviruses/geneticsABSTRACT
Russian HIV variants with common (rapid/high SI and slow/low NSI) and rare (slow/low SI and rapid/high NSI) phenotypes are described. SI variants demonstrate a higher p24 concentration level than serotype-independent NSI. The majority of SI variants belonging to A, B, and C serotypes were isolated from patients with mainly stage B3. At stages B2 and C2, when the majority of viruses are characterized by NSI phenotype, serotype D isolates are characterized only by SI phenotype. The spectrum of cell tropism was wide for all rapid/high strains and narrow for all slow/low ones.
