Evaluation of the Efficacy of Disinfectant Footmats for the Reduction of Bacterial Contamination on Footwear in a Large Animal Veterinary Hospital.

BACKGROUND: Infection control is critical to providing high-quality patient care. Many veterinary teaching hospitals (VTHs) utilize footbaths or footmats at entrances and key control points throughout the facility to decrease trafficking of pathogenic microorganism on contaminated footwear. HYPOTHESIS/OBJECTIVES: To compare efficacy of 4 disinfectants used in footmats for decreasing bacterial contamination of footwear in a large animal hospital. ANIMALS: A single adult dairy cow was housed in a stall for 4 days to facilitate stall contamination with fecal material. METHODS: Overboots were experimentally contaminated with organic material in a standardized manner. Each boot was randomly assigned to 1 of 5 treatments (no treatment, or exposure to 1 of 4 disinfectants: an accelerated peroxygen [AHP], a peroxygen [VIRKON], a quaternary ammonium [QUAT], and a phenolic disinfectant [PHENOLIC]) by stepping on a soaked footmat and collecting samples from boot soles. Generalized linear modeling was used to analyze differences in bacterial counts. RESULTS: Reductions in colony-forming units (CFUs) on treated boots ranged from no detectable reduction to 0.45 log10 and varied by disinfectant. Percentage reductions in total bacterial counts generally were larger (albeit still modest) for AHP and QUAT disinfectants (range 37-45%) and smallest for the PHENOLIC (no detectable reduction). CONCLUSIONS AND CLINICAL IMPORTANCE: In general, use of disinfectant footmats was associated with significant reductions in viable bacteria on overboots-albeit with variable efficacy. Footmats may be useful adjuncts to cleaning and disinfection programs for decreasing trafficking of microorganisms throughout VTHs but should not be considered as a sole prevention method.

Comparison of disinfectant efficacy when using high-volume directed mist application of accelerated hydrogen peroxide and peroxymonosulfate disinfectants in a large animal hospital.

REASONS FOR PERFORMING STUDY: Effective decontamination of animal holding environments is critical for providing high quality patient care and maintaining a safe working environment. Disinfection of animal holding environments is a significant challenge during times of epidemic disease. OBJECTIVES: The purpose of this study was to evaluate the disinfectant efficacy of 3 strategies for high-volume directed mist application of accelerated hydrogen peroxide and peroxymonosulfate disinfectants; 4.25% accelerated hydrogen peroxide (Accel(®) ; AHP) at a 1:16 dilution and single and double applications of 2% peroxymonosulfate solution (Virkon-S(®) ; VIR-1 and VIR-2) for decontamination of a large animal hospital environment. STUDY DESIGN: Experiment. METHODS: After cleaning and disinfection of the hospital environment, transparencies experimentally contaminated with known concentrations of Staphylococcus aureus, Salmonella enterica and Pseudomonas aeruginosa were placed on vertical surfaces. Disinfectant solution was applied by directed mist application and, after 30 min of contact time, transparencies were collected and individually placed into tubes containing 10 ml Dey-Engley broth. The process was repeated for each disinfectant. Tenfold dilutions of each sample were plated onto tryptic soy blood agar with 5% sheep blood. Bacterial counts from transparencies exposed to disinfectants were compared with counts from control transparencies (unexposed to disinfectants) to evaluate reduction in colony forming units. RESULTS: The least squares mean reduction (log10 ) in colony forming units (CFUs) for S. aureus and P. aeruginosa was 1.5-2.5 logs and approximately 0.8-1.0 logs for S. enterica. Reductions were generally largest for VIR-2 and smallest for AHP, although these differences were not all statistically significant and the magnitude of differences may not be clinically relevant. CONCLUSIONS: For the organisms evaluated, all 3 disinfectants applied as a directed mist were effective at reducing CFUs in a veterinary hospital environment. Effective disinfection using this method of application is dependent on adequate cleaning prior to application, and use of adequate volumes of disinfectant.

Effectiveness of cattle operated bump gates and exclusion fences in preventing ungulate multi-host sanitary interaction.

Tuberculosis (TB) is endemic in Eurasian wild boar (Sus scrofa) and red deer (Cervus elaphus) in south central Spain, where evidence suggests transmission to domestic cattle. Known risk factors for TB at the interface between livestock and wild ungulate species include density and spatial overlap, particularly around waterholes during summer. We evaluated the effectiveness of selective exclusion measures for reducing direct and indirect interaction between extensive beef cattle and wild ungulates at waterholes as an alternative for the integrated control of TB. We first monitored 6 water points (WP) with infrared-triggered cameras at a TB positive cattle farm to quantify interactions. We then assigned 3 WP to be "cattle-only" and 3 to be "wildlife-only". Cattle-only WP were surrounded with a wildlife-proof fence (2.5 m high) and an original design of cattle-specific gate. Wildlife-only WP were surrounded by a fence that wild ungulates could breach but cattle could not (1.2 m high). Red deer, roe deer (Capreolus capreolus) and wild boar easily jumped or undercrossed this fence. Wildlife-only fences were 100% effective in preventing cattle access to WP and did not impede wildlife use. Many cows learned to operate the cattle-specific gate quickly and others followed and learned from them. Within 2 weeks, around 70% of cows actively entered and exited through the cattle-specific gate. We demonstrate how simple, low-cost fencing strategies can serve as biosecurity measures to substantially reduce direct and indirect contact between cattle and wild ungulates, serving to reduce the potential for TB transmission. Our designs can be used in the context of integral plans to mitigate disease transmission between cattle and wildlife, and have potential for protecting or segregating the use of a variety of resources in different contexts.