The effect of structural white matter abnormalities on the clinical course of epilepsy.

OBJECTIVES: The aim of this study was to assess the impact of the extent of brain white matter lesions on the development of cognitive and psychoemotional disorders, and to investigate correlations between the degree of integration of brain pathway structures and the clinical features of epilepsy. MATERIAL AND METHODS: Forty-six epileptic patients (36 with pharmacoresistant epilepsy and 10 who had been in remission for over a year) and 10 normal volunteers (the control group) were examined. To evaluate diffusion tensor MRI findings, the index of fractional anisotropy (FA) and index of apparent diffusion coefficient (ADC) were used. For an intergroup comparison of DTI data, the Mann-Whitney test was used, criterion; correlation analysis was performed using the Spearman rank correlation coefficient. The threshold of statistical significance was set at р < 0.05. RESULTS: A significant difference was noted in the ADC data on the side of the epileptic focus in the patients in persistent remission as compared to the pharmacoresistant patients (р < 0.05). No differences were found between the patient groups' fractional anisotropy data. In cases of mesial temporal sclerosis in patients with pharmacoresistant epilepsy, a "weakening" of the tractography pattern in the opposite hemisphere was found (r = 0.66, p = 0.0005). Decreases in the tracts appearing in brain temporal lobes was typical of patients with pharmacoresistant forms of epilepsy (r = 0.46, p = 0.0005). A pathological decrease in FA and an increase in ADC correlated with the results on the Beck scale and the Spielberger-Khanin anxiety scale (r = -0.2, p < 0.001) as well as with P300 peak latency data (r = 0.23, p < 0.001). Analyses of the peculiarities of EEG patterns and FA data demonstrated a correlation between the existence of epileptic activity and a decrease in FA (r = -0.7, t = -2.44, p = 0.01). CONCLUSIONS: Microstructural brain matter changes make it possible to assess the course of epilepsy to predict the outcomes of medicamental correction of paroxysmal states.

Electronically excited states of membrane fluorescent probe 4-dimethylaminochalcone. Results of quantum chemical calculations.

Quantum-chemical calculations of ground and excited states for membrane fluorescent probe 4-dimethylaminochalcone (DMAC) in vacuum were performed. Optimized geometries and dipole moments for lowest-lying singlet and triplet states were obtained. The nature of these electronic transitions and the relaxation path in the excited states were determined; changes in geometry and charge distribution were assessed. It was shown that in vacuum the lowest existed level is of (n, π*) nature, and the closest to it is the level of (π, π*) nature; the energy gap between them is narrow. This led to an effective (1)(π, π*) →(1)(n, π*) relaxation. After photoexcitation the molecule undergoes significant transformations, including changes in bond orders, pyramidalization angle of the dimethylamino group, and planarity of the molecule. Its dipole moment rises from 5.5 Debye in the ground state to 17.1 Debye in the (1)(π, π*) state, and then falls to 2 Debye in the (1)(n, π*) state. The excited (1)(n, π*) state is a short living state; it has a high probability of intersystem crossing into the (3)(π, π*) triplet state. This relaxation path explains the low quantum yield of DMAC fluorescence in non-polar media. It is possible that (3)(π, π*) is responsible for observed DMAC phosphorescence.