ABSTRACT
Cystic hydatid disease (Hydatidosis) is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. Hydatidosis in man (as an accidental host) is caused by infection with the ova containing larval stage of Echinococcus granulosus. In the last decade, different techniques have been employed for sero-diagnosis of hydatidosis; as IHA, IFA, ELISA, CCLE (Counter Current Immuno-electrophoresis). This paper evaluated the validity of ELISA and IHA. Since whole hydatid cyst fluid was used as a source of antigen for serodiagnosis. Thirty surgical and pathological hydatidosis proven patients were examined. The sensitivity and specificity of ELISA were 96.7% & 97.5% respectively, and that of IHA were 86.7%, &95% respectively.
Subject(s)
Echinococcosis/blood , Echinococcosis/diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Hemagglutination Tests/methods , Serologic Tests/methods , Adult , Antibodies, Protozoan , Female , Humans , MaleABSTRACT
Serum lipids, liver enzymes and total bile acids were measured in chronic Fasciola (cholestatic and non-cholestatic) patients. Variations in the biochemical parameters between infected and control groups were detected and typified by considerably higher serum triglycerides (TG, P < 0.001), alkaline phosphatase (ALP, P< 0.001) and total bile acids (TBA, P < 0.001) in the infected group. For cholestatic patients, TG, very low density lipoprotein (VLDL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gammaglutamyl transferase (GGT) and ALP were statistically higher (P < 0.001 for each comparison) and total cholesterol (TC); low density lipoprotein (LDL) and high density lipoprotein (HDL), were statistically lower than non-cholestatic patients (P = 0.02, < 0.001, < 0.001 respectively). No statistically significant differences were found for the TC, HDL, VLDL, ALT, AST and GGT between non-cholestatic patients and healthy controls.
