ABSTRACT
Incidence of unusually high numbers of stillbirths was observed at a piggery unit at the Veterinary University research farm in Tamil Nadu State of India. Systematic examination of the tissue from stillborn piglets led to the identification of presence of Porcine circovirus 2 (PCV2). Detailed analysis utilizing electron microscopy, polymerase chain reaction and sequencing confirmed the presence of PCV2 in the tissue of affected piglets. Histopathology analysis of the affected piglet tissue showed lymphoid cell depletion of lymphnodes, spleen and infiltration of liver, kidney, myocardium, etc. Retrospective examination of the morbidity and mortality history in the farm revealed high mortality in young and weanling piglets suggestive of PCV2 infection-induced diseases. This is the first report of emergence of major disease incidence in farmed swine due to PCV2 infection in India.
Subject(s)
Circoviridae Infections/veterinary , Circovirus/physiology , Stillbirth/veterinary , Swine Diseases/epidemiology , Animals , Circoviridae Infections/epidemiology , Circoviridae Infections/virology , Circovirus/genetics , Genes, Viral , Incidence , India/epidemiology , Microscopy, Electron, Transmission/veterinary , Open Reading Frames , Organ Specificity , Phylogeny , Polymerase Chain Reaction/veterinary , Retrospective Studies , Sequence Analysis, DNA/veterinary , Stillbirth/epidemiology , Swine , Swine Diseases/virologyABSTRACT
This report describes Newcastle disease in peacock and the isolation and characterization of the virus. The virus had an intracerbral pathogenicity index of 1.71 and mean death time of 47 h. The isolate had multiple basic amino acids at the fusion protein cleavage site sequence ((110)GGRRQRRFIG(119)) with a phenylalanine at residue 117. Biological and molecular characterization revealed that the virus is velogenic. Phylogenetic analysis placed the isolate in genotype II.
Subject(s)
Galliformes , Newcastle Disease/virology , Newcastle disease virus/classification , Animals , Genotype , Newcastle Disease/pathology , Newcastle disease virus/genetics , PhylogenySubject(s)
Adenoviridae Infections/veterinary , Aviadenovirus/pathogenicity , Bird Diseases/epidemiology , Coturnix , Pericardial Effusion/veterinary , Adenoviridae Infections/epidemiology , Animals , Disease Outbreaks/veterinary , Inclusion Bodies, Viral , Liver/virology , Pericardial Effusion/epidemiology , Pericardium/pathology , Pericardium/virology , SyndromeABSTRACT
A tumor mass was identified below the shoulder region of a 5-year-old male rhesus monkey (Macaca mulatta). The mass was excised and diagnosed as sebaceous gland adenoma based on the microscopic findings. Morphologically it appeared as an elevated, dome-shaped, circumscribed mass of 3.6 x 2.8 x 3.2 cm in dimension with tan speckled color. Histologically, the tumor was composed of mature, sebaceous cells (sebocytes), basal cells arranged in a mass of irregular shapes and sizes, with a characteristic appearance of poly or multilobular structure. Sebocytes were well differentiated with foamy cytoplasm in the center of the lobules and poorly or undifferentiated densely staining basal cells in the periphery of the lobules. Cellular changes in the adjacent lymph node included hyperplasia of plasma cells, macrophages and lymphoid elements with typical mitosis.
