Subject(s)
Bone Neoplasms/diagnosis , Osteosarcoma/diagnosis , Scapula , Adult , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Bone Neoplasms/therapy , Combined Modality Therapy , Humans , Male , Osteosarcoma/therapy , Reoperation , Scapula/diagnostic imaging , Scapula/pathology , Tomography, X-Ray ComputedABSTRACT
The antitumor effects of non-steroidal anti-inflammatory drugs, tenoxicam and piroxicam, against sarcoma 180 cells cultured in 7-week-old male mice were examined in vitro under ultrasonic irradiation. The survival rate of tumor cells when tenoxicam or piroxicam was added to sarcoma 180 suspension under ultrasonic irradiation was significantly lower than that when ultrasound alone was applied. Furthermore, when L-histidine, a scavenger of singlet oxygen and hydroxyl radical, or D-mannitol, a scavenger of hydroxyl radical, was used concurrently, the survival rate of tumor cells was significantly higher with L-histidine. From the above findings, it is surmised that tenoxicam and piroxicam increase the antitumor effects of ultrasound by increasing the production of singlet oxygen and other active oxygen species.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Cell Survival/radiation effects , Piroxicam/analogs & derivatives , Piroxicam/pharmacology , Sarcoma 180/pathology , Ultrasonic Therapy , Animals , Cell Survival/drug effects , Equipment Design , Humans , Male , Mice , Mice, Inbred ICR , Tumor Cells, Cultured , Ultrasonic Therapy/instrumentation , Ultrasonic Therapy/methodsABSTRACT
The antitumor effect of intra-tumoral injection of Cepharanthin, a biscoclaurin alkaloid extracted from Stephania cephalanta Hayata, and staphylococcal enterotoxin B was evaluated using F344 male rats bearing transplantable rat osteosarcoma, S-SLM. A macroscopic lung metastatic nodule of tumor was transplanted into the subcutaneous back space, and 0.5 mg of Cepharanthin and 2 pg of staphylococcal enterotoxin B were injected into the tumor on days 12, 13 and 14. On day 28, all animals were killed with an overdose of pentobarbital sodium, and the transplanted tumors and lungs were examined. The wet weight of the lungs of the rats treated with Cepharanthin and staphylococcal enterotoxin B was significantly lower, and apoptosis in the lung metastatic nodules was significantly higher than that of the control or that of rats treated with only Cepharanthin or staphylococcal enterotoxin B. In the transplanted tumors, infiltration of TRAP (tartrate-resistant acid phosphatase)-positive multinucleated giant cells was prominent in the rats treated with Cepharanthin and staphylococcal enterotoxin B. These findings indicate that intra-tumoral injection of Cepharanthin and staphylococcal enterotoxin B induced infiltration of TRAP-positive multinucleated giant cells within the transplanted rat osteosarcoma, and reduced lung metastasis.
Subject(s)
Alkaloids/therapeutic use , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Enterotoxins/therapeutic use , Lung Neoplasms/prevention & control , Osteosarcoma/prevention & control , Acid Phosphatase/analysis , Animals , Benzylisoquinolines , In Situ Nick-End Labeling , Isoenzymes/analysis , Lung Neoplasms/enzymology , Lung Neoplasms/genetics , Lung Neoplasms/secondary , Male , Neoplasm Transplantation , Organ Size , Osteosarcoma/enzymology , Osteosarcoma/genetics , Osteosarcoma/pathology , Rats , Rats, Inbred F344 , Tartrate-Resistant Acid PhosphataseABSTRACT
STUDY DESIGN: Pseudohypoparathyroidism associated with disorders of the spine is rarely reported. In this report, the authors present a case of pseudohypoparathyroidism in a 41-year-old man who had narrow spinal canal and multiple disc herniation in the cervical spine. SUMMARY OF BACKGROUND DATA: The patient had progressive spastic tetraplegia due to cervical cord compression caused by multiple disc herniations at the C3-C4, C4-5, and C5-C6 levels in the developmentally narrow spinal canal. Based on the features of Albright's osteodystrophy, serum laboratory results, and an Ellsworth-Howard test, the diagnosis of pseudohypoparathyroidism, type I was confirmed. METHODS: The patient underwent expansive laminoplasty at C3 through C6 levels. RESULTS: His symptoms have gradually improved during the 1-year and 6-month periods after the surgery. CONCLUSIONS: A case of pseudohypoparathyroidism-associated spinal stenosis was reported.
