ABSTRACT
Temozolomide is used commonly in the treatment of some types of cancers, but it may also result in cognitive impairments such as memory deficits. l -Dopa, a well known medicine for the central nervous system, has been shown to have positive effects on some cognitive disorders. Here we sought to investigate the effect of l -Dopa on temozolomide-induced cognitive impairments. BALB/c mice were subjected to 3-days temozolomide and 6-days concomitant l -Dopa/benserazide administration in six groups (control, l -Dopa 25â mg/kg, l -Dopa 75â mg/kg, temozolomide, temozolomideâ +â l -Dopa 25â mg/kg, and temozolomideâ +â l -Dopa 75â mg/kg). Open field test, object location recognition, novel object recognition test, and shuttle-box test were carried out to determine the locomotor, anxiety-like behavior, and memory function of subjects. TNF-α and brain-derived neurotrophic factor (BDNF) gene expression in the hippocampus was measured by real-time PCR. Mice treated with temozolomide showed recognition memory impairment, along with hippocampal TNF-α and BDNF mRNA expression level raise, and detection of histological insults in hematoxylin and eosin hippocampal slides. Mice that received temozolomideâ +â l -Dopa showed normal behavioral function and lower TNF-α and BDNF hippocampal mRNA expression levels, and histologically normal hippocampal CA1 region in comparison with mice in the temozolomide group. Our results provide evidence that l -Dopa prevents temozolomide-induced recognition memory deficit in mice at the acute phase probably via l -Dopa antineuroinflammatory effects.
Subject(s)
Brain-Derived Neurotrophic Factor , Cognitive Dysfunction , Mice , Male , Animals , Temozolomide/pharmacology , Temozolomide/metabolism , Brain-Derived Neurotrophic Factor/metabolism , Tumor Necrosis Factor-alpha/metabolism , Tumor Necrosis Factor-alpha/pharmacology , Cognitive Dysfunction/chemically induced , Cognitive Dysfunction/drug therapy , Cognitive Dysfunction/metabolism , Hippocampus , Memory Disorders/chemically induced , Memory Disorders/drug therapy , Memory Disorders/metabolism , RNA, Messenger/metabolismABSTRACT
This letter is written according to stoichiometric calculation doubt about the article entitled "effects of carbonated liquids on oropharyngeal swallowing measures in people with neurogenic dysphagia" by Sdravou et al.
