Increase of pro-oxidants with no evidence of lipid peroxidation in exhaled breath condensate after a 10-km race in non-athletes.

It is a well-established fact that exercise increases pro-oxidants and favors oxidative stress; however, this phenomenon has been poorly studied in human lungs. Pro-oxidative generation (H(2)O(2), NO(2) (-)), lipid peroxidation markers (MDA), and inflammation (pH) in exhaled breath condensate (EBC) have been determined through data from 10 active subjects who ran 10 km; samples were obtained immediately before, at 20, and at 80 min post-exertion. In EBC, the concentration of H(2)O(2) at 80 min post-exertion was increased. NO(2) (-) concentration showed a tendency to increase at 80 min post-exertion, with no variations in MDA and pH. No variations of NO(2) (-) were found in plasma, while there was an increase of NO(2) (-) at 80 min post-exertion in the relation between EBC and plasma. NO(2) (-) in EBC did not correlate to plasmatic NO(2) (-), while it did correlate directly with H(2)O(2) in EBC, suggesting a localized origin for the exercise-related NO(2) (-) increase in EBC. MDA in plasma did not increase nor correlate with MDA in EBC. In conclusion, high-intensity exercise increases lung-originated pro-oxidants in non-athlete subjects with no evidence of early lipid peroxidation and changes in the pH value in EBC.

Lung oxidative stress as related to exercise and altitude. Lipid peroxidation evidence in exhaled breath condensate: a possible predictor of acute mountain sickness.

Lung oxidative stress (OS) was explored in resting and in exercising subjects exposed to moderate and high altitude. Exhaled breath condensate (EBC) was collected under field conditions in male high-competition mountain bikers performing a maximal cycloergometric exercise at 670 m and at 2,160 m, as well as, in male soldiers climbing up to 6,125 m in Northern Chile. Malondialdehyde concentration [MDA] was measured by high-performance liquid chromatography in EBC and in serum samples. Hydrogen peroxide concentration [H(2)O(2)] was analysed in EBC according to the spectrophotometric FOX(2) assay. [MDA] in EBC of bikers did not change while exercising at 670 m, but increased from 30.0+/-8.0 to 50.0+/-11.0 nmol l(-1) (P<0.05) at 2,160 m. Concomitantly, [MDA] in serum and [H(2)O(2)] in EBC remained constant. On the other hand, in mountaineering soldiers, [H(2)O(2)] in EBC under resting conditions increased from 0.30+/-0.12 mumol l(-1) at 670 m to 1.14+/-0.29 mumol l(-1) immediately on return from the mountain. Three days later, [H(2)O(2)] in EBC (0.93 +/-0.23 mumol l(-1)) continued to be elevated (P<0.05). [MDA] in EBC increased from 71+/-16 nmol l(-1) at 670 m to 128+/-26 nmol l(-1) at 3,000 m (P<0.05). Changes of [H(2)O(2)] in EBC while ascending from 670 m up to 3,000 m inversely correlated with concomitant variations in HbO2 saturation (r=-0.48, P<0.05). AMS score evaluated at 5,000 m directly correlated with changes of [MDA] in EBC occurring while the subjects moved from 670 to 3,000 m (r=0.51, P<0.05). Lung OS may constitute a pathogenic factor in AMS.

[The elderly at risk in the province of Guadalajara]. / El anciano de riesgo en la provincia de Guadalajara.

OBJECTIVE: To study fragile elderly people in the population, their characteristics and their distribution according to fragility markers. DESIGN: Cross-sectional, descriptive study using a survey. SETTING: Primary care. Elderly people living in Guadalajara in January 2002. PARTICIPANTS: 434 people were interviewed (1.24% of population). There were 157 losses (26.6%). INTERVENTIONS: The questionnaire contained social and demographic variables (age, sex, setting), care variables (medication, pathologies, home care) and evaluative scales (Barthel, Folstein, Yesavage, Diaz-Palacios). MAIN MEASUREMENTS: Fragility markers: 3 or more pathologies, 6 or more drugs, dementia, and/or positive on one of the four scales used. RESULTS: Subjects lived in rural areas more (56.7%; CI, 52-61.3), but there were not more women. Fragile elderly, n=257 (59.2%; CI, 54.6-63.8), were more women (OR=1.8; CI, 1.5-2.2) and over 70 (OR=80-84, 5.2; CI, 3.7-7.5; OR=85-89, 8.2; CI, 5.3-12.8). Prevalence of markers was: 3 or more pathologies, 30.2% (95% CI, 25.8-34.5); cognitive deterioration, 22.6% (95% CI, 18.7-26.5); social risk, 20.8% (95% CI, 16.3-23.8); multi-medication, 18.7% (95% CI, 15-22.3); Barthel incapacity, 11.7 (95% CI, 8.7-14.8); depression, 5.3% (95% CI, 3.2-7.5); and dementia, 3.2% (95% CI, 1.6-4.9). Women suffered cognitive deterioration more. The over-80s suffered cognitive deterioration, social risk and incapacity more. CONCLUSIONS: There was high prevalence of fragility with cognitive deterioration, multi-medication and social risk; and lower presence of dementia. Women and the most elderly people were most affected.

Calcitriol improves streptozotocin-induced diabetes and recovers bone mineral density in diabetic rats.

Vitamin D analogs exert a preventative effect on experimental diabetes, but whether or not they are able to halt progress of established diabetes is not yet known. Moreover, it is widely accepted that diabetes may induce osteoporosis, but the efficacy of vitamin D on diabetic osteoporosis is not clear. In order to help clarify these issues, we have tested the efficacy of calcitriol streptozotocin-induced diabetes. Streptozotocin (60 mg/Kg body weight) was injected in 3-month-old Wistar rats, randomly distributed into two groups: vehicle (olive oil) treated diabetic rats (D) and diabetic rats treated with 1.25-(OH)2D3 250 mg, three times a week (DT). Control animals (C) were treated with vehicle alone. The experiment lasted 8 weeks. The histology of the pancreata was evaluated. Blood glucose and calcium and phosphate in serum and urine were measured. Finally, bone mineral density (BMD) of tibia and lumbar vertebrae were evaluated. After 8 weeks, diabetes persisted in 85% of the diabetic rats (D group), but in only 45% of vitamin D-treated group (DT). At the end of the experiment, DT animals were separated into two groups, those still remaining diabetic (DT-NR) and reversed animals (DT-R). Moreover, bone loss was observed in diabetic animals (D), whereas BMD of DT-R rats showed similar values to those of controls (C). Our results suggest that 1.25(OH)2D3 improves diabetes and, as such, may recover BMD in streptozotocin-induced diabetic rats.

Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation.

We further investigated the role of the Arabidopsis CBF regulatory genes in cold acclimation, the process whereby certain plants increase in freezing tolerance upon exposure to low temperature. The CBF genes, which are rapidly induced in response to low temperature, encode transcriptional activators that control the expression of genes containing the C-repeat/dehydration responsive element DNA regulatory element in their promoters. Constitutive expression of either CBF1 or CBF3 (also known as DREB1b and DREB1a, respectively) in transgenic Arabidopsis plants has been shown to induce the expression of target COR (cold-regulated) genes and to enhance freezing tolerance in nonacclimated plants. Here we demonstrate that overexpression of CBF3 in Arabidopsis also increases the freezing tolerance of cold-acclimated plants. Moreover, we show that it results in multiple biochemical changes associated with cold acclimation: CBF3-expressing plants had elevated levels of proline (Pro) and total soluble sugars, including sucrose, raffinose, glucose, and fructose. Plants overexpressing CBF3 also had elevated P5CS transcript levels suggesting that the increase in Pro levels resulted, at least in part, from increased expression of the key Pro biosynthetic enzyme Delta(1)-pyrroline-5-carboxylate synthase. These results lead us to propose that CBF3 integrates the activation of multiple components of the cold acclimation response.

Prevalencia de la infección tuberculosa en el medio escolar de Guadalajara / Prevalence of tuberculosis infection in the school age in Guadalajara, Spain

Objetivos: Estimar la prevalencia de infección tuberculosa en los niños escolarizados en los cuatro primeros cursos de Educación Primaria, y analizar su distribución según su clase social y la existencia de antecedentes de enfermedad. Métodos: Se trata de un estudio transversal mediante la aplicación de prueba diagnóstica y autocumplimentación de encuesta en una muestra representativa de la población escolar de la provincia de Guadalajara en 1997-1998 (diseño muestral polietápico). Se aplicó la prueba de la tuberculina utilizando 2 UT del lote PPD-RT 23, según normas de consenso y lectura por el método de Sokal. La clasificación de ocupaciones y la definición de clase social se hizo según la actualización de la Sociedad Española de Epidemiología. Resultados: Se estudiaron 1.093 niños (50,8 por ciento varones) comprendidos entre 6 y 9 años de edad. La prevalencia total de infectados fue de 1,64 por ciento (IC: 1-2,3). La distribución de infectados no mostró diferencias significativas para el estrato urbano-rural, antecedentes familiares, clase social, superficie de la vivienda y tipo de actividad ocupacional desarrollada por los padres. La prevalencia aumentó en los cursos superiores y en los niños de mayor edad, siendo de 0,4 por ciento (IC: 0,01-1,3) en el primer curso y de 3,53 por ciento (IC:2,4-4,6) en el último. En los nacidos en 1988 la prevalencia fue de 3,23 (IC: 1,2-5,1), y de 0,73 (IC: 0-1,7), 1,88 (IC: 0,2-3,51), y 0,4 (IC: 0-1,2) en los nacidos en 1989, 1990 y 1991, respectivamente. Conclusiones: La infección tuberculosa es poco prevalente en nuestro medio escolar en relación a estudios de otras comunidades. Esta baja frecuencia y el tamaño muestral utilizado han impedido la estimación estadística de posibles diferencias en la distribución de infectados en algunos subgrupos estudiados, aunque se puede objetivar una mayor afectación en los más mayores (AU)

Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression.

Cold-induced expression of the Arabidopsis COR (cold-regulated) genes is mediated by a DNA regulatory element termed the CRT (C-repeat)/DRE (dehydration-responsive element). Recently, we identified a transcriptional activator, CBF1, that binds to the CRT/DRE and demonstrated that its overexpression in transgenic Arabidopsis plants at non-acclimating temperatures induces COR gene expression and increases plant freezing tolerance. Here we report that CBF1 belongs to a small family of closely related proteins which includes CBF2 and CBF3. DNA sequencing of an 8.7 kb region of the Arabidopsis genome along with genetic mapping experiments indicated that the three CBF genes are organized in direct repeat on chromosome 4 at 72.8 cM, closely linked to molecular markers PG11 and m600. Like CBF1, both CBF2 and CBF3 activated expression of reporter genes in yeast that contained the CRT/DRE as an upstream activator sequence. The transcript levels for all three CBF genes increased within 15 min of transferring plants to low temperature, followed by accumulation of COR gene transcripts at about 2 h. CBF transcripts also accumulated rapidly in response to mechanical agitation. The promoter regions of the CBF genes do not contain the CRT sequence, CCGAC, and overexpression of CBF1 did not have a detectable effect on CBF3 transcript levels, suggesting that the CBF gene family is not subject to autoregulation. We propose that cold-induced expression of CRT/DRE-containing COR genes involves a low temperature-stimulated signalling cascade in which CBF gene induction is an early event.

[Epidemiology of leprosy through the study of frequency of visits to the Trillo Specialized Hospital in 1943-1995]. / Epidemiología de la lepra a través del estudio de la frecuentación en el hospital especializado de Trillo durante el período 1943-1995.

BACKGROUND: The purpose of this study is to describe the social-demographic and clinical profile of hospitalised leprosy patients and to check whether typology has changed during the history of the centre. METHODS: Descriptive and retrospective study, performed by means of reviewing a sample of the patient records registered at the Centre since it was founded in 1943 until 1995. 366 cases were chosen by means of a systematic random sample and questionnaires completed on social-demographic and clinical variables. RESULTS: Most of the patients were male (71.9%, IC: 67.3-76.4), young (39.6 years of age, IC: 37.9-41.4), single (46.2%, IC: 41-51.3%) with low levels of education (illiteracy: 54.1%, IC: 40-69) with occupations relating to farming (35.5%, IC: 30.6-40.4) from Southern Spanish regions (patients from Andalucía 52.8%, IC: 45.8-54). The disease showed a family background in 31.1% of cases (IC: 26.4-35.9) and serious multibacillary forms (Lepromatose Leprosy 66.1%, IC: 61.2-71), which affected lower limbs in 72.1% of cases, heads in 63.1% and upper limbs in 64.4%. Global mortality of people admitted to hospital was 31.1% (IC: 26.4-35.9). During the surveyed period, patient age increased, and symptoms and mortality decreased. In general, hospital stays were for long periods (7.1 years, IC: 6.1-8.1), although at the end of the surveyed period, stays decreased considerably, in accordance with the duration of treatment (2.2 years). CONCLUSIONS: A Young male, in a precarious financial situation living in the South of Spain appear to be the patient profile which, with severe initial anatomical symptoms and later favourable results may represent the disease in a country with an autocuthonous endemy and characteristic epidemiology, which is in the pre-eradication phase.