Regulation of anthocyanin synthesis in red lettuce in plant factory conditions: A review.

Anthocyanins, natural pigments known for their vibrant hues and beneficial properties, undergo intricate genetic control. However, red vegetables grown in plant factories frequently exhibit reduced anthocyanin synthesis compared to those in open fields due to factors like inadequate light, temperature, humidity, and nutrient availability. Comprehending these factors is essential for optimizing plant factory environments to enhance anthocyanin synthesis. This review insights the impact of physiological and genetic factors on the production of anthocyanins in red lettuce grown under controlled conditions. Further, we aim to gain a better understanding of the mechanisms involved in both synthesis and degradation of anthocyanins. Moreover, this review summarizes the identified regulators of anthocyanin synthesis in lettuce, addressing the gap in knowledge on controlling anthocyanin production in plant factories, with potential implications for various crops beyond red lettuce.

Efficacy and safety of intravenous paracetamol in management of labour pains in a low resource setting: a randomized clinical trial.

OBJECTIVE: To assess the use of single dose of paracetamol intravenously in management of labour pains. BACKGROUND: Pain during labour is a complex, subjective and multi-faceted physiological phenomenon that varies in intensity among women and is subjected to many social and cultural modifiers. SUBJECTS AND METHODS: This randomized clinical study was conducted in Obstetrics and gynecology department from March 2019 to March 2020 including 96 primiparous women randomized into paracetamol group (n = 48) received 1000 mg of paracetamol IV infusion and pethidine group (n = 48) received 50 mg of pethidine given slowly IV. Primary outcome is the change of the intensity of perceived labor pain. Pain score was followed and recorded by visual analogue scale (VAS). Our study protocol was registered at ClinicalTrials.gov; NCT04744727. RESULTS: VAS score was highly significant improved gradually after 30 min, 1, 2 and 3 h of paracetamol and pethidine taken compared at start study, but participants in paracetamol group had lower pain after 2 and 3 h (3.92 ± 1.42 and 5.69 ± 1.07) than those of the pethidine groups (4.42 ± 1.87 and 5.38 ± 1.34). Also, 2.1% of paracetamol group developed dizziness and 4.2% developed nausea and vomiting, while there was 29.2% of pethidine group developed dizziness and 37.5% developed nausea and vomiting. CONCLUSIONS: Intravenous paracetamol as labour analgesia is effective, safe, inexpensive, available and with no maternal or fetal side effects as compared to Pethidine. Paracetamol needs to have more chance in comparison to other forms as a labour pain analgesia, especially in our communities.

Dental age assessment of 3-15-year-old Saudi children and adolescents using Demirjian's method-A radiographic study.

Dental age assessment plays a pivotal role in clinical practice, demographic studies, forensics, and courts of law but is affected by ethnic and geographic variations. The aim was to determine the population-specific weighted scores needed when dental age is estimated using Demirjian's method for Saudi children and adolescents between the ages of 3 and 15 years. Design: A total of 298 panoramic radiographs were collected from Saudi Arabia. Dental age was assessed using Demirjian's method (1973). Chronological age was determined from the date of birth and the date on which the panoramic radiograph was taken for each individual. Between 3- and 15-years age group, the Saudi boys had an estimated age of 9.07 ± 1.96 years and chronological age of 8.49 ± 2.30 years. The Saudi girls had an estimated age of 9.22 ± 1.93 years and chronological age of 8.78 ± 2.32 years. With Demirjian's method, the Saudi boys were 0.57 ± 1.48 years, and girls were 0.44 ± 1.66 years ahead of their chronological ages (p < .05). New population-specific weighted scores were developed to convert dental age according to Demirjian's method into estimated ages in the contemporary Saudi Arabian population. This study can be used for further research and comparisons with other population groups, regions or communities.

Utero-placental perfusion Doppler indices in growth restricted fetuses: effect of sildenafil citrate.

OBJECTIVE: To assess efficacy and tolerability of sildenafil citrate on utero-placental blood flow and fetal growth in pregnancies complicated by fetal growth restriction (FGR). METHODS: From March 2015, a randomized controlled trial of 54 patients at 24 weeks or more complicated by FGR and abnormal Doppler indices were randomly allocated 1:1 into an intervention arm (receive sildenafil citrate, 50 mg) or a control arm (receive placebo). The primary outcomes were changes occurred in the Doppler parameters 2 h following drug administration. RESULTS: Baseline characteristics were similar between groups. Significant difference was observed in the Delta uterine and umbilical Doppler indices among sildenafil group as compared to placebo group (p < 0.001). Middle cerebral Doppler indices, however, decreased significantly after sildenafil, which could be the result of shifting more blood to improve the utero-placental perfusion. No difference regarding Delta cerebro-placental ratio among both groups (p = 0.979). Sildenafil was also associated with pregnancy prolongation (p = .0001), increased gestational age at delivery (p = .004), improved neonatal weight (p = .0001), and less admission to neonatal intensive care unit (p = .03). No adverse effects reported in both treatment arms. CONCLUSION: Sildenafil citrate, by its vasodilator effect, can improve utero-placental blood flow in pregnancies complicated by FGR and abnormal Doppler. CLINICAL TRIAL: gov Registry: NCT02362399.

The relationship between IL-18 and atherosclerotic cardiovascular risk in Egyptian lean women with polycystic ovary syndrome.

BACKGROUND: Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder among women of reproductive age. The evidence in support of low-grade inflammation in PCOS as an etiology is emerging. Inflammation is likely to be associated with other prominent aspects of PCOS including insulin resistance (IR) and cardiovascular disease (CVD) risk. Interleukin-18 (IL-18) is considered as a strong marker of inflammation. OBJECTIVES: Evaluation of the relation between serum IL-18 and atherosclerotic CVD (ASCVD) risk in Egyptian lean females with PCO. METHODS: This study included control group of healthy lean normally menstruating females, lean PCOS group (BMI < 25 kg/m2), and obese PCOS group (BMI > 25 kg/m2) presented with infertility and diagnosed according to Rotterdam criteria. Measurements of serum lipid profile, IR, and IL-18 were done. RESULTS: Lipid accumulation product (LAP), IR and ASCVD risk were significantly higher in PCOS patients (lean and obese) compared to controls and in obese compared to lean. Serum IL-18 was significantly higher in the PCOV groups compared to the controls and correlated directly with LAP, IR and ASCVD risk. CONCLUSION: IL-18 is elevated in PCOS patients even in lean ones and is correlated with IR and ASCVD risk.

Uterine packing versus Foley's catheter for the treatment of postpartum hemorrhage secondary to bleeding tendency in low-resource setting: A four-year observational study.

OBJECTIVE: To assess the effectiveness and safety of uterine packing versus Foley's catheter tamponade for controlling postpartum hemorrhage (PPH) secondary to bleeding tendency after vaginal delivery. METHODS: This was a prospective observational study conducted on 92 patients with primary PPH due to bleeding tendency following vaginal delivery who were unresponsive to uterotonics and bimanual compression of the uterus. Patients were divided into two groups, Uterine packing group (n = 45) and Foley catheter group (n = 47). The primary outcome was the success rate of the procedure. Secondary outcome addressed the maternal complications. RESULTS: The use of uterine packing resulted in stoppage of active bleeding in 93.3% of cases compared to only 68.1% in the Foley's catheter group (p < 0.05). Although the rate of minor complications namely fever, pain and urinary complaints were higher in the uterine packing group, it does not reach to a significant difference when compared to the Foley's catheter (p > 0.05). Six cases who failed to Foley catheter tamponade underwent emergency hysterectomy with no cases in the uterine packing group. CONCLUSIONS: The use of uterine packing to arrest PPH is simple, quick and safe procedure to avoid further surgical interventions and to preserve the fertility in low-resource setting.

Amniotic fluid index and estimated fetal weight for prediction of fetal macrosomia: a prospective observational study.

OBJECTIVE: The purpose of this study was to assess the value of combining the estimated fetal weight (EFW) and amniotic fluid index (AFI) measured in term patients early in labor with intact membranes for prediction of macrosomia. METHODS: In a single center, prospective observational study, 600 patients in the first stage of labor before rupture of membranes in whom ultrasonography was performed to measure AFI and EFW, and these data were analyzed statistically to evaluate prediction of fetal macrosomia. RESULTS: Macrosomia occurred in 64 cases (10.6%). The AFI was significantly higher in the macrosomic group (p = 0.001). It was noted that the area under receiver operating characteristic (ROC) curves for EFW was 0.93 and that of AFI was 0.67. Based on suggested combined EFW and AFI cutoffs of 4000 g and 164 mm, respectively, the positive predictive value (PPV) for combined parameters (92.3%) was higher than that of EFW (75%) and that of AFI (27%) and the likelihood ratio for combination (93.7%) was higher than that of EFW (24.7%) and that of AFI (21%). CONCLUSION: Combined use of EFW and AFI improves prediction of macrosomia at birth rather than the EFW alone.

Value of first-trimester serum lipid profile in early prediction of preeclampsia and its severity: A prospective cohort study.

STUDY OBJECTIVE: The purpose of this study was to evaluate the association of first-trimester serum lipid profile with preeclampsia (PE) and if so, its association with the severity of the disease. STUDY: Prospective cohort study. PATIENT AND METHODS: A total of 251 pregnant women participated in this study in their first trimester. Maternal blood samples were collected between 4 and 12 weeks of gestation. Fasting serum triglycerides (TGs), total cholesterol (TC), HDL-cholesterol, and low-density lipoprotein cholesterol (LDL-C) were drawn and measured by ELISA. From the cohort, 26 women developed PE (16 mild and 10 severe) and 174 remained normotensive and used as control subjects. RESULTS: There was a significant rise in fasting serum TGs, TC, and LDL-C and a significant decrease in HDL-C in women who developed PE than in those who remained normotensive and more significantly evident in severe preeclamptic cases compared to mild cases of PE (p < 0.05). CONCLUSION: Early pregnancy dyslipidemia, particularly hypertriglyceridemia and hypercholesterolemia, may help in predicting women who later develop PE and may help in prediction of its severity.

Impact of unilateral versus bilateral laparoscopic ovarian drilling on ovarian reserve and pregnancy rate: a randomized clinical trial.

OBJECTIVE: To assess the impact of unilateral dose adjusted ovarian drilling (ULOD) compared to bilateral ovarian drilling (BLOD) on ovarian reserve and pregnancy rate. METHODS: This randomized clinical study included 105 patients with polycystic ovary syndrome. Patients were assigned to two groups; group 1 (n = 52) underwent dose adjusted ULOD using 60 Joules/cm(3) applied to the larger ovary, while group 2 (n = 53) underwent BLOD with fixed doses of 1200 J. Ovulation rate, anti-Mullerian hormone (AMH), antral follicle count (AFC), and pregnancy rates were assessed at 3 and 6 months of follow-up. RESULTS: Ovulation and pregnancy rates at 3 months periods were comparable (p > 0.05), but was significantly higher in BLOD at 6 months period (p < 0.05). There was a highly significant difference between the two groups regarding the AMH level at 3- and 6-month follow-up periods (p < 0.001) with lower levels in the BLOD group. The AFC was comparable in the two groups after 3 months (p > 0.05) but became higher in the ULOD at 6-month follow-up period (p < 0.001). CONCLUSION: Dose-adjusted ULOD applied to the larger ovary has comparable ovulation and pregnancy rates to fixed dose BLOD at 3-month follow-up periods with decrease in its effectiveness after 6 months.

Quantitative analysis of survivin in colorectal adenocarcinoma: increased expression and correlation with telomerase activity.

The aims of the present study are to quantitatively analyze survivin expression, its clinicopathologic roles, and correlation with telomerase activity in a large cohort of patients with colorectal adenocarcinoma. Real-time polymerase chain reaction was used to quantitate expression level of survivin messenger RNA and human telomerase reverse transcriptase messenger RNA (telomerase activity) in 51 patients with colorectal adenocarcinomas. The findings were correlated with the clinicopathologic features of patients, which were prospectively collected into a computerized database. Survivin messenger RNA was expressed in all tumor samples. The level of expression in tumor tissues was increased in comparison with matched nontumor mucosa in the same patient (P = .01). The level of expression of survivin was significantly correlated with the level of human telomerase reverse transcriptase expression (P = .008) and size of the colorectal adenocarcinomas (P = .004). Survival of the patients with colorectal adenocarcinoma was associated with the TNM stages (P = .001) and not with the level of expression of survivin. Thus, survivin activity was altered in colorectal adenocarcinoma. The high prevalence of survivin expression and correlation with telomerase activity are important factors for consideration in gene targeting therapy for colorectal adenocarcinoma.

Real-time PCR quantification of human telomerase reverse transcriptase (hTERT) in colorectal cancer.

AIMS: Human telomerase reverse transcriptase (hTERT) is believed to a reliable marker for telomerase activity. The expression of telomerase activity has not been investigated in a consecutive series of patients with colorectal adenocarcinoma in North Queensland. The objective of this study was to evaluate the significance of hTERT mRNA expression in colorectal adenocarcinoma in North Queensland. METHODS: Matched samples of tumour and adjacent non-tumorous mucosa samples from 53 colorectal carcinomas and nine colorectal adenomas were collected. In all these samples, RNA was extracted and then transcribed to cDNA. Real-time polymerase chain reaction (RT-PCR) was used to quantitate expression the level of hTERT mRNA. The findings were correlated with the clinicopathological features of patients with these tumours prospectively collected into a computerised database. RESULTS: hTERT mRNA was expressed in all tumour samples. The level of expression in the colorectal adenocarcinomas was significantly higher than the corresponding non-tumorous mucosa (p = 0.009, t-test). The level of expression in the adenocarcinomas was slightly higher than those of adenomas, but the difference was not statistically significant. A higher level of hTERT expression was often noted in the adenocarcinomas arising from the left colon and rectum when compared with those from the right colon (p = 0.029). CONCLUSIONS: Colorectal adenocarcinoma revealed expression of telomerase hTERT mRNA, which was detected quantitatively by real-time PCR. hTERT could be a potential biomarker for colorectal cancer. The difference between proximal and distal colorectum in hTERT expression could account for their known different clinical behaviour.

Fiber microaxicons fabricated by a polishing technique for the generation of Bessel-like beams.

We report a simple method for generating microaxicons at the extremity of commercial optical fibers. The proposed solution, based on a polishing technique, can readily produce any desired microaxicon cone angle and is independent of the nature of the fiber. An optical study of microaxicon performance, in terms of confinement ability and length of the generated Bessel-like beams, is presented as a function of the microaxicon angle. This study, made possible by the experimental acquisition of the 3D light distribution of the Bessel-like beams, reveals the relationship between the Bessel-like beam confinement zone and the beam length. Finally, the effect of diffraction of the Bessel-like beams, induced by the limited lateral extent of the incident fiber mode, is studied and discussed.

Telomerase activity of basal cell carcinoma in patients living in North Queensland, Australia.

Basal cell carcinoma (BCC) is the most common skin cancer in North Queensland, Australia. The role of telomerase in BCC has not been investigated in this region. The objective of the study was to investigate the expression levels of human telomerase reverse transcriptase (hTERT) and telomerase activity in nodular BCCs and superficial BCCs from patients living in North Queensland. Matched tumor and adjacent nontumorous mucosa samples from 12 BCCs (7 nodular and 5 superficial) among 11 male patients were collected. In these samples, RNA was extracted and then transcribed to complementary DNA. Real-time polymerase chain reaction (PCR) was used to quantify the expression levels of hTERT messenger RNA (mRNA). In addition, telomeric repeat amplification protocol assay, a PCR-based method, was performed to detect telomerase activity in tissue extracts from these samples. The findings were correlated with the clinicopathologic features of patients with these tumors. All BCC samples expressed telomerase hTERT mRNA, and 75% (9/12) of the BCC samples showed telomerase activity. The mean level of expression among BCC samples was higher than that among matched nontumor samples (mean, 0.468 versus 0.140; P < .0001). Nodular BCC samples showed higher expression levels of hTERT mRNA as compared with superficial BCC samples (mean, 0.584 versus 0.305; P < .05). There was no significant association between expression of hTERT mRNA and tumor size. In conclusion, this study is the first on telomerase expression in BCC in Queensland as well as the first to analyze telomerase in BCC by quantitative PCR. The results indicate that telomerase alterations have an important role in the pathogenesis of BCCs. A unique finding is that the telomerase expression level in nodular BCCs is different from that in superficial BCCs.

A novel carbonic anhydrase from the giant clam Tridacna gigas contains two carbonic anhydrase domains.

This report describes the presence of a unique dual domain carbonic anhydrase (CA) in the giant clam, Tridacna gigas. CA plays an important role in the movement of inorganic carbon (Ci) from the surrounding seawater to the symbiotic algae that are found within the clam's tissue. One of these isoforms is a glycoprotein which is significantly larger (70 kDa) than any previously reported from animals (generally between 28 and 52 kDa). This alpha-family CA contains two complete carbonic anhydrase domains within the one protein, accounting for its large size; dual domain CAs have previously only been reported from two algal species. The protein contains a leader sequence, an N-terminal CA domain and a C-terminal CA domain. The two CA domains have relatively little identity at the amino acid level (29%). The genomic sequence spans in excess of 17 kb and contains at least 12 introns and 13 exons. A number of these introns are in positions that are only found in the membrane attached/secreted CAs. This fact, along with phylogenetic analysis, suggests that this protein represents the second example of a membrane attached invertebrate CA and it contains a dual domain structure unique amongst all animal CAs characterized to date.