Enhancement of Benzene Emissions in Special Combinations of Electronic Nicotine Delivery System Liquid Mixtures.

Electronic nicotine delivery systems (ENDS) are battery-powered devices introduced to the market as safer alternatives to combustible cigarettes. Upon heating the electronic liquid (e-liquid), aerosols are released, including several toxicants, such as volatile organic compounds (VOCs). Benzene has been given great attention as a major component of the VOCs group as it increases cancer risk upon inhalation. In this study, several basic e-liquids were tested for benzene emissions. The Aerosol Lab Vaping Instrument was used to generate aerosols from ENDS composed of different e-liquid combinations: vegetable glycerin (VG), propylene glycol (PG), nicotine (nic), and benzoic acid (BA). The tested mixtures included PG, PG + nic + BA, VG, VG + nic + BA, 30/70 PG/VG, and 30/70 PG/VG + nic + BA. A carboxen polydimethylsiloxane fiber for a solid-phase microextraction was placed in a gas cell to trap benzene emitted from a Sub-Ohm Minibox C device. Benzene was adsorbed on the fiber during the puffing process and for an extra 15 min until it reached equilibrium, and then it was determined using gas chromatography-mass spectrometry. Benzene was quantified in VG but not in PG or the 30/70 PG/VG mixtures. However, benzene concentration increased in all tested mixtures upon the addition of nicotine benzoate salt. Interestingly, benzene was emitted at the highest concentration when BA was added to PG. However, lower concentrations were found in the 30/70 PG/VG and VG mixtures with BA. Both VG and BA are sources of benzene. Enhanced emissions, however, are mostly noticeable when BA is mixed with PG and not VG.

Aromatic Hydrocarbon Receptor Repressor (AHRR) is a biomarker of ambient air pollution exposure and Coronary Artery Disease (CAD).

Two hundred and twenty subjects were recruited while undergoing cardiac catheterization. AHRR cg05575921 methylation was shown to be significantly decreased in ever smokers compared to never smokers (Mean± SD = 64.2 ± 17.2 vs 80.1 ± 11.1 respectively; P < 0.0001). In addition, higher urinary levels of 2-OHNAP and 2-OHFLU were significantly associated with more AHRR cg05575921 hypomethylation, even after correcting for smoking (ß[95%CI]= -4.161[-7.553, -0.769]; P = 0.016 and -5.190[-9.761, -0.618]; P = 0.026, respectively) but not 1-OHPYR (ß[95%CI]= -3.545 [-10.935, 3.845]; P = 0.345). Additionally, hypomethylation of AHRR ROI was significantly associated with obstructive coronary artery disease (CAD) after adjusting for smoking, age, sex, diabetes and dyslipidemia (OR [95%CI] = 1.024[1.000 - 1.048]; P = 0.046). Results of this study necessitate further validation to potentially consider clinical incorporation of AHRR methylation status as an early predictive biomarker for the potential association between ambient air pollution and CAD.

Effects of Aftermarket Electronic Cigarette Pods on Device Power Output and Nicotine, Carbonyl, and ROS Emissions.

Aftermarket pods designed to operate with prevalent electronic nicotine delivery system (ENDS) products such as JUUL are marketed as low-cost alternatives that allow the use of banned flavored liquids. Subtle differences in the design or construction of aftermarket pods may intrinsically modify the performance of the ENDS device and the resulting nicotine and toxicant emissions relative to the original equipment manufacturer's product. In this study, we examined the electrical output of a JUUL battery and the aerosol emissions when four different brands of aftermarket pods filled with an analytical-grade mixture of propylene glycol, glycerol, and nicotine were attached to it and puffed by machine. The aerosol emissions examined included total particulate matter (TPM), nicotine, carbonyl compounds (CCs), and reactive oxygen species (ROS). We also compared the puff-resolved power and TPM outputs of JUUL and aftermarket pods. We found that all aftermarket pods drew significantly greater electrical power from the JUUL battery during puffing and had different electrical resistances and resistivity. In addition, unlike the case with the original pods, we found that with the aftermarket pods, the power provided by the battery did not vary greatly with flow rate or puff number, suggesting impairment of the temperature control circuitry of the JUUL device when used with the aftermarket pods. The greater power output with the aftermarket pods resulted in up to three times greater aerosol and nicotine output than the original product. ROS and CC emissions varied widely across brands. These results highlight that the use of aftermarket pods can greatly modify the performance and emissions of ENDS. Consumers and public health authorities should be made aware of the potential increase in the level of toxicant exposure when aftermarket pods are employed.

Poor regulation implications in a low and middle income country based on PAH source apportionment and cancer risk assessment.

Ambient particle-bound polycyclic aromatic hydrocarbons (PAHs) were collected for one year at an urban background site, and spatially and temporally compared to yearly averages in three coastal cities in Lebanon. The samples were quantified using gas chromatography-mass spectrometry (GC-MS) and source apportioned with an optimized robust method using positive matrix factorization (PMF). Three major sources were found to contribute to PAH emissions at the urban background site, namely, traffic (48%), diesel generators (23%), and incineration (29%). The cancer risk was found higher than what was measured at the same site in previous years with an increase of 35%. Improper regulations of the sources (incineration, power plant, diesel generators and traffic) identified in the different sites resulted in PAH intraurban variability. It is essential to study the chemical components of particulate matter (PM) in order to assess toxicity. In particular, particle-bound PAHs and their oxidation products are known for their carcinogenicity as well as their persistence in the atmosphere, which facilitate their transport to new locations. In the absence of law enforcement, unregulated sources and their total contribution to ambient PAHs present a major health risk. This calls for the attention of development funding agencies and their need to implement sustainable "carbon-free" funding strategies in support of urban development of low and middle-income countries (LMICs).

Flavor-Toxicant Correlation in E-cigarettes: A Meta-Analysis.

Flavors in electronic cigarette (ECIG) liquids may increase ECIG aerosol toxicity via intact distillation or chemical transformation. For this report, we performed a meta-analysis of the literature to categorize the compounds found in flavored ECIG liquids into a few chemical classes and to predict their possible chemical transformations upon ECIG liquid aerosolization. This analysis allowed us to propose specific correlations between flavoring chemicals and aerosol toxicants. A literature search was conducted in November 2019 using PubMed. Keywords included terms related to ECIGs and flavors. Studies were included if they reported chemical ingredients of flavored liquids and clearly stated the commercial names of these liquids. The obtained data were visualized on a network diagram to show the common chemical compounds identified in flavored ECIG liquids and categorize them into different chemical classes. The systematic literature review included a total of 11 articles. Analysis of the data reported gave a total of 189 flavored liquids and 173 distinct chemical compounds that were categorized into 22 chemical classes according to their functional groups. The subsequent prediction of chemical transformations of these functional groups highlighted the possible correlation of flavor compounds to aerosol toxicants.

Vaped Humectants in E-Cigarettes Are a Source of Phenols.

Electronic cigarettes (ECIGs) have always been promoted as safer alternatives to combustible cigarettes. However, a growing amount of literature shows that while ECIGs do not involve combustion-derived toxicants, thermal degradation of the main constituents of ECIG liquid produces toxicants such as carbonyls. In this study, we report the detection of phenolic compounds in ECIG aerosols using a novel analytical method. The introduced method relies on liquid-liquid extraction to separate phenols from the major constituents of ECIG aerosol: propylene glycol (PG) and vegetable glycerol (VG). Phenol emissions from ECIGs were tested at different powers, puff durations, PG/VG ratios, nicotine benzoate concentrations, and flow rates to assess the influence of these operating parameters on phenol formation. The performance metrics showed that the analytical method has high specificity and reliability to separate and quantify phenolic compounds in ECIG aerosols. Increasing power and puff duration significantly increased all phenol emissions, while flow rate had no significant effects. The phenol profile in the ECIG aerosol was dominated by the unsubstituted phenol that reached comparable levels to those of IQOS, combustible cigarettes, and waterpipe. In contrast, low levels of the more toxic phenolic compounds, like catechol and hydroxyquinone, were quantified in ECIG aerosols. Emission of toxicants is presented, for the first time in this study, as the yield per unit of time, or flux (µg/s), which is more suitable for interstudy and interproduct comparison. This work demonstrates a robust analytical method for isolating and quantifying phenol emissions in ECIG aerosols. Using this method, the study shows that phenols, which are not present in the simple solution of nicotine benzoate dissolved in mixtures of PG/VG, are formed upon vaping. Phenol emissions are independent of the nicotine benzoate concentration but significantly correlated with the PG/VG ratio. Emissions increased with power and puff duration, consistent with conditions that lead to a higher temperature and greater thermal degradation.

Does the Bubbler Scrub Key Toxicants from Waterpipe Tobacco Smoke?: Measurements and Modeling of CO, NO, PAH, Nicotine, and Particulate Matter Uptake.

Waterpipe tobacco smoking is a global epidemic. A persistent perception among users is that the water bubbler filters the smoke, reducing its risk profile. The objectives of this study were to quantify the purported filtering effect by comparing toxicant yield when a waterpipe was machine smoked with and without the smoke passing through the water bubbler. We found that the water bubbler did not reduce CO, NO, polycyclic aromatic hydrocarbons (PAHs), or dry particulate matter (DPM) yields but did reduce nicotine and carbonyl compounds (CCs) yields by approximately 50%. These mixed results were consistent with theoretical simulations of the mass transport processes involved.

Comparison of CO, PAH, Nicotine, and Aldehyde Emissions in Waterpipe Tobacco Smoke Generated Using Electrical and Charcoal Heating Methods.

Waterpipe tobacco smoking (WTS) has been characterized as a global epidemic. Waterpipe smoke has been shown to contain and deliver significant doses of many of the toxicants known to cause cancer, respiratory, and cardiovascular diseases in cigarette smokers. It has also been shown that the charcoal used to heat the tobacco contributes most of the polycyclic aromatic hydrocarbons (PAHs) and carbon monoxide (CO) found in the smoke, two major causative agents in smoking-related lung cancer and heart disease, respectively. Possibly as a result of growing awareness of charcoal as a toxicant source, electrical heating elements (EHEs) are being marketed for waterpipe use as reduced harm charcoal substitutes. We measured thermal performance characteristics (tobacco burned, total aerosolized particulate matter) and toxicant emissions in WTS generated using three commercially available waterpipe EHEs and charcoal to examine the hypothesis that EHEs can function similarly to charcoal while presenting a reduced toxicant profile. Toxicants quantified included total particulate matter, nicotine, PAHs, CO, and volatile aldehydes delivered at the mouthpiece when the waterpipe was machine smoked using a standard protocol. We found that while EHEs involved an 80% reduction in total PAH and a 90% reduction in CO emissions, they also resulted in a several-fold increase in the potent respiratory toxicant acrolein. These mixed findings underscore the complexity of toxicant reduction by product manipulation and suggest that marketing EHEs as reduced harm products may be misleading.

Carbon Monoxide and Small Hydrocarbon Emissions from Sub-ohm Electronic Cigarettes.

Electronic cigarettes (ECIGs) are routinely advertised as a safer alternative to combustible cigarettes. ECIGs have been shown to emit less toxicants than conventional cigarettes. This study presents for the first time the mouthpiece emissions of carbon monoxide (CO) and small hydrocarbon gases, in addition to carbonyls, from a rebuildable atomizer sub-ohm device (SOD). Because ECIGs do not involve combustion, CO emissions are commonly thought to be a negligible component of ECIG aerosols. CO exposure is a major causative agent of heart disease among smokers. Aerosol generated by vaping a solution of propylene glycol and glycerol was collected in a small chamber. The gas phase was then directed for analysis to a long-path gas cell of a Fourier transform infrared instrument under reduced pressure. The effects of power, ECIG heating coil material, and coil geometry on the generation of small gases were assessed. Results showed that small gases, including CO, carbon dioxide, methane, ethylene, and acetylene, were detected in SOD-emitted gases. Electrical power and material of construction significantly affected the concentrations of the emitted gases. Nickel metal wire was more reactive than kanthal, nichrome, and stainless steel. Depending on use patterns and device operation, users of SOD devices may be exposed daily to similar levels of CO as are cigarette smokers. This finding casts doubt on the validity of CO as a biomarker to distinguish ECIG from tobacco cigarette use and suggests that some subset of ECIG users may be at risk from CO-related heart disease.

Toxic emissions resulting from sucralose added to electronic cigarette liquids.

Electronic cigarettes (ECIGs) are appealing in part because of the many flavors of the liquids used in them. Concerns have been raised that some ECIG liquid flavors, especially those that are sweet, are attracting otherwise nicotine-naïve youth to ECIGs. Sucralose is an artificial, non-caloric sweetener that is added to some ECIG liquids. In this study, we evaluated the toxicants, namely isomers of chloropropanols that can be produced when sucralose-containing ECIG liquid is aerosolized. An analytical separation method relying on solid-phase extraction (SPE) to isolate chloropropanols from the propylene glycol/glycerol matrix was developed. Chloropropanols were then derivatized by silylation before they were analyzed on GC-MS. The influence of different ECIG operating conditions on the generation of chloropropanols was studied by varying ECIG device design and power output and also the sucralose concentration of the liquid. Heated sucralose-containing ECIG liquids produce two toxic compounds that can be found in the resulting aerosols. The two chloropropanols, 3-monochloro-1,2-propanediol (3-MCPD) and 1,3-dichloropropanol (1,3-DCP) that were detected under all conditions were found to be correlated significantly with liquid sucralose content. Effective regulation of ECIGs will minimize user and bystander exposure to these and other ECIG toxicants.

Reactive Oxygen Species Emissions from Supra- and Sub-Ohm Electronic Cigarettes.

Electronic cigarettes (ECIGs) are battery-powered devices that heat and vaporize solutions containing propylene glycol (PG) and/or vegetable glycerin (VG), nicotine and possible trace flavorants to produce an inhalable aerosol. The heating process can lead to the formation of reactive oxygen species (ROS), which are linked to various oxidative damage-initiated diseases. Several studies in the literature have addressed ROS emissions in ECIG aerosols, but the effects of power, ECIG device design and liquid composition on ROS are relatively unknown. In addition, ROS emissions have not been examined in the emerging high power, sub-Ohm device (SOD) category. In this study, an acellular 2',7'-dichlorofluorescin (DCFH) probe technique was optimized to measure ROS in ECIG aerosols. The technique was deployed to measure ROS emissions in SOD and supra-Ohm ECIGs while varying power, heater coil head design and liquid composition (PG/VG ratio and nicotine concentration). Liquids were made from analytical standards of PG, VG and nicotine and contained no flavorants. At high powers, ROS emissions in ECIGs and combustible cigarettes were similar. Across device designs, ROS emissions were uncorrelated with power (R2 = 0.261) but were highly correlated with power per unit area (R2 = 0.78). It was noticed that an increase in the VG percentage in the liquid yielded higher ROS flux, and nicotine did not affect ROS emissions. ROS emissions are a function of device design and liquid composition at a given power. For a given liquid composition, a promising metric for predicting ROS emissions across device designs and operating conditions is power per unit area of the heating coil. Importantly, ROS formation is significant even when the ECIG liquid consists of pure analytical solutions of PG and VG; it can therefore be viewed as intrinsic to ECIG operation and not solely a by-product of particular flavorants, contaminants or additives.

Fate of pyrazines in the flavored liquids of e-cigarettes.

Popularity of electronic cigarettes (ECIGs) has increased tremendously among young people, in part due to flavoring additives in ECIG liquids. Pyrazines are an important class of these additives, and their presence in tobacco cigarettes has been correlated with increased acceptability of smoking among smokers and bystanders. Pyrazine use by the tobacco industry is therefore thought to encourage smoking. However, the extent of transfer of pyrazines present in the liquid to aerosols upon vaping remains unclear. We present a simple analytical method to quantify six pyrazine derivatives in liquids and aerosols of ECIGs that allows the isolation of pyrazines from interfering compounds, like nicotine. Standard pyrazine solutions and commercial ECIG samples of different brands and flavors were tested for their pyrazine content in the liquids and in the generated aerosols from these solutions. Testing on ECIG commercial liquids revealed a heterogeneous distribution in the levels and types of pyrazines, with acetyl and alkyl pyrazines present in more than 70% of the samples. This method confirmed that pyrazine additives are common in ECIG and that labels do not usually reflect the type and quantity of pyrazines in the liquid. Pyrazines were not correlated with the nicotine content or the brand of the liquid. The aerosols showed similar pyrazine profiles to their corresponding liquids. The efficiency of transfer of pyrazines into the particle phase was approximately 46%. Therefore, addition of pyrazines to ECIGs should be regulated, because they act synergistically with nicotine to increase product appeal, ease smoking initiation, and discourage cessation.