Antitumor effect of algae silver nanoparticles on human triple negative breast cancer cells.

In recent years, metallic nanoparticles have gained increasing attention due to their prospective applications in the field of nanomedicine, with increasing research into their use in cancer therapy. In this current research, we investigated the effect of green synthesized Silver Nanoparticles (AgNPs) capped with Noctiluca scintillans algae extract. The phytochemicals present in the shell of AgNPs were identified using GC-MS. Different compounds with anticancer activity such as n-hexadecanoic acid, beta-sitosterol, stigmasterol and palmitic acid were detected among others. The effects of Algae-AgNPs synthesized were tested on MDA-MB-231 human breast cancer cells and HaCat human keratinocyte normal cells. Cell viability assay revealed a time and dose-dependent effect against breast cancer cells with a less potent effect against normal cells. The cell viability reduction is not attributed to a cytotoxic nor an antiproliferative effect of the Algae-AgNPs as attested by LDH release and BrdU incorporation. Algae-AgNPs exhibited an exceptional ability to specifically induce apoptosis in cancer cells and not normal cells. The observed effects are not attributed to the AgNPs, as demonstrated by the lack of impact of the Starch-AgNPs (used as a negative control) on cell survival and apoptosis. In addition to that, we show that Algae-AgNPs significantly reduced tumor cell migration by downregulation of matrix metalloprotease-9 levels. In vivo, the breast cancer xenograft model showed a significant reduction of tumor growth in mice treated with Algae-AgNPs. These findings highlight the promising potential of the green synthesized AgNPs as a safe targeted therapy for cancer treatment.

An Overview of Metabolomics Studies Based on Qatari Population.

The aim of metabolomics research is to identify the metabolites that play a role in various biological traits and diseases. This scoping review provides an overview of the current state of metabolomics studies that focus on the Qatari population. Our findings indicate that few studies have been conducted on this population, with a focus on diabetes, dyslipidemia, and cardiovascular disease. Blood samples were the primary source of metabolite identification, and several potential biomarkers for these diseases were proposed. To the best of our knowledge, this is the first scoping review that presents an overview of metabolomics studies in Qatar.

Applications of Artificial Intelligence (AI) in Medical Education: A Scoping Review.

Artificial Intelligence (AI) is increasingly used to support medical students' learning journeys, providing personalized experiences and improved outcomes. We conducted a scoping review to explore the current application and classifications of AI in medical education. Following the PRISMA-P guidelines, we searched four databases, ultimately including 22 studies. Our analysis identified four AI methods used in various medical education domains, with the majority of applications found in training labs. The use of AI in medical education has the potential to improve patient outcomes by equipping healthcare professionals with better skills and knowledge. Post-implementation refers to the outcomes of AI-based training, which showed improved practical skills among medical students. This scoping review highlights the need for further research to explore the effectiveness of AI applications in different aspects of medical education.

The 14-Kilodalton Human Growth Hormone Fragment a Potent Inhibitor of Angiogenesis and Tumor Metastasis.

The 14-kilodalton human growth hormone (14 kDa hGH) N-terminal fragment derived from the proteolytic cleavage of its full-length counterpart has been shown to sustain antiangiogenic potentials. This study investigated the antitumoral and antimetastatic effects of 14 kDa hGH on B16-F10 murine melanoma cells. B16-F10 murine melanoma cells transfected with 14 kDa hGH expression vectors showed a significant reduction in cellular proliferation and migration associated with an increase in cell apoptosis in vitro. In vivo, 14 kDa hGH mitigated tumor growth and metastasis of B16-F10 cells and was associated with a significant reduction in tumor angiogenesis. Similarly, 14 kDa hGH expression reduced human brain microvascular endothelial (HBME) cell proliferation, migration, and tube formation abilities and triggered apoptosis in vitro. The antiangiogenic effects of 14 kDa hGH on HBME cells were abolished when we stably downregulated plasminogen activator inhibitor-1 (PAI-1) expression in vitro. In this study, we showed the potential anticancer role of 14 kDa hGH, its ability to inhibit primary tumor growth and metastasis establishment, and the possible involvement of PAI-1 in promoting its antiangiogenic effects. Therefore, these results suggest that the 14 kDa hGH fragment can be used as a therapeutic molecule to inhibit angiogenesis and cancer progression.