Clinical evaluation of the perception of post-trauma paresthesia in the mandible, using a biomimetic material: A preliminary study in humans.

There is a great effort from numerous research groups in the development of materials and therapeutic strategies for the functional recovery of patients who have suffered peripheral nerve injuries (PNI). In an article in vivo, the formation of a nerve bridge was observed, reconnecting the distal and proximal stumps, in the sciatic nerve of rats, indicating the effective participation of the biomaterial in the recovery of peripheral nerve injuries. For the current pilot study, 15 cases of multiple fractures of the mandible, with involvement of the inferior alveolar nerve (IAN) were selected and studied: JC (control cases) n = 6 with conventional treatment, and JT (treated cases) n = 9, with the use of biomimetic biomaterial. The evaluation of the return to sensitivity was measured through a self-assessment, where the patients assigned scores from 0 to 10, where zero (0) represented the complete absence of sensitivity and ten (10) the normality of the perception of local sensitivity. Patients were evaluated from the preoperative period to the 360th day. The statistical results obtained by the t-Student, Shapiro-Wilk normality and non-parametric One-Way ANOVA tests indicated statistically significant differences (p < 0.005; 0.005 e 0.5 respectively), between the two treatments, which were reflected in the clinical results observed, we also calculate the size of the effect represented by ϵ2, calculated by Cohen's d. The results indicate a great difference between the treatments performed,ϵ2 = 1.00. In the 6 cases followed up in the JC group, four remained with a significant deficit until the end of the evaluations and two indicated the remission of the lack of sensitivity in this period. In the JT group, in 28 days, all cases indicated complete remission of the lack of sensitivity with healing concentration. In one of the cases where there was a complete rupture of the mental nerve, the (score-10) was observed in 60 days. The observed results indicate the existence of a statistical significance between the groups and an important relationship when using the biomimetic biomaterial during the recovery of the perception of sensitivity in polytraumatized patients, compatible with the results observed in laboratory animals, which may indicate its clinical feasibility in the reduction of sequelae in PNI.

Which oilseed meal provides a higher yield and better nutrient profile of milk in grazing cows?

This study aims to verify which of the oilseed meals will provide a higher milk production efficiency and milk nutrient profile in grazing cows to optimize the production system and improve milk nutrients for the benefit of nutrition and human health. A Latin square experiment was conducted with 12 cows for 84 days, fed Tanzania grass, and concentrated with cottonseed meal (CotM), sunflower meal (SunM), soybean meal (SoyM) and peanut meal (PeaM). SunM tended to have a higher production of 3.5% fat milk and higher milk urea nitrogen compared with the other treatments. SunM tended to have higher amount of fat in the milk compared with SoyM. PeaM tended to have lower fatty acids C13:0, C15:0 anteiso, C15:0 in milk compared with SunM. PeaM tended to have lower C16:0 and C17:0 iso compared with SoyM and CotM. PeaM had lower C18:1trans in comparison to the other meals. The addition of SoyM tended to have higher C18:1 cis15 compared with PeaM and tended to have lower thrombogenicity index compared with CotM and PeaM. Aiming at milk health in pasture production systems, SoyM presented the best nutritional quality of the lipid fraction. For productivity and fat yield, SunM had better results.

Paternal exposure to bupropion affects postnatal development in the offspring.

The aim of this study was to evaluate whether paternal exposure to bupropion hydrochloride (BUP), an inhibitor of dopamine and noradrenaline reuptake, would affect the postnatal development of offspring. Male mice were divided into a BUP-treated (40mgkg-1day-1 by gavage, 45 days) or control (saline by gavage, 45 days) group (n=20 in each group). From Day 35 to Day 45 of treatment, males were allowed to mate with drug-naïve female mice. Postnatal development of the offspring (both sexes) was evaluated from Postnatal day (PND) 1 to PND60. Physical development parameters (weight gain, body length, incisor eruption, pinna detachment), anogenital distance, vaginal opening, reflexes (palmar grasp, surface righting, negative geotaxis and adult gait) and some behavioural parameters (locomotor activity and anxiety-like behaviour) were altered in the offspring of BUP-treated males. The results demonstrate that paternal exposure to BUP induces long-lasting changes in the postnatal development of the offspring.

CHARACTERIZATION OF CASSAVA (MANIHOT SCULENTA CRANTZ) AERIAL PARTS FOR RUMINANT FEEDING

The objective of this research was to characterize the chemical composition and digestibility of aerial part and cuttings of four cassava cultivars grown in five different levels of soil fertilization and three plant densities, in order to use the residues for ruminant feeding. The experiment was conducted using a randomized complete block design, in strip-split-plot scheme, with four replicates. The plots were composed of IAC14, IAC15, IAC90, and Cascuda cultivars; the subplots consisted of five fertilization categories, one fertilization with poultry litter (3000 kg.ha-1) and four chemical fertilization rates (0, 150, 450, 900 kg.ha-1) with NPK 4-20-20; and with the sub-subplots consisted of three plant densities (7,500, 12,500, 17,500 plants.ha-1). During the experiment, aerial part and cuttings were sampled for chemical and in vitro digestibility analyzes. There were differences between the varieties of cassava and fertilization rates on fiber fractions, chemical composition, in vitro digestibility, total digestible nutrients (TDN), and dry matter yield (kg / ha) of aerial part (P

Characterization of cassava (Manihot sculenta crantz) aerial parts for ruminant feeding / Caracterização da parte aérea de mandioca para alimentação animal

The objective of this research was to characterize the chemical composition and digestibility of aerial part and cuttings of four cassava cultivars grown in five different levels of soil fertilization and three plant densities, in order to use the residues for ruminant feeding. The experiment was conducted using a randomized complete block design, in strip-splitplot scheme, with four replicates. The plots were composed of IAC14, IAC15, IAC90, and Cascuda cultivars; the subplots consisted of five fertilization categories, one fertilization with poultry litter (3000 kg.ha-1 ) and four chemical fertilization rates (0, 150, 450, 900 kg.ha-1 ) with NPK 4-20-20; and with the sub-subplots consisted of three plant densities (7,500, 12,500, 17,500 plants.ha-1 ). During the experiment, aerial part and cuttings were sampled for chemical and in vitro digestibility analyzes. There were differences between the varieties of cassava and fertilization rates on fiber fractions, chemical composition, in vitro digestibility, total digestible nutrients (TDN), and dry matter yield (kg / ha) of aerial part (P < 0.05) and cuttings (P < 0.05). Cassava aerial part and cuttings have considerable amounts of nutrients that can be used for ruminant feeding on small farms and for animal categories with low nutritional requirements. From all treatments, variety IAC15, with chemical fertilization of 900 kg.ha-1, as well as fertilization with poultry litter, with the highest plant density, were those with better nutritional composition and greater amount of available nutrients.

O objetivo desta pesquisa foi quantificar e estudar a composição química e a digestibilidade da parte aérea e da manivamãe de quatro cultivares de mandioca, cultivadas sob cinco diferentes níveis de adubações e três densidades de plantio, com a finalidade de utilização destes coprodutos na alimentação de ruminantes. O experimento foi realizado no Pólo Regional de Desenvolvimento do Médio Paranapanema utilizando o delineamento experimental em blocos casualizados, em esquema de parcelas sub-subdivididas, com quatro repetições. As parcelas foram compostas pelas cultivares IAC 14, IAC 15, IAC 90 e Cascuda; as subparcelas compostas por doses de 4-20-20 (0, 150, 450, 900 kg/ha) ou adubação com cama de frango (3.000 kg/ha de cama de frango) e as sub-subparcelas compostas por densidades de plantio de 7.500, 12.500, 17.500 plantas/ha. Foram feitas amostragens de parte aérea e maniva-mãe e realizadas análises químicas para determinação dos nutrientes e digestibilidade in vitro. Houve diferença entre as variedades de mandioca e níveis de fertilização sobre as frações da fibra; composição química; digestibilidade in vitro; nutrientes digestíveis totais (NDT), e rendimento de matéria seca (kg/ha) da parte aérea (P < 0,05) e da maniva-mãe (P < 0,05). A parte aérea e a maniva-mãe oriundas das sobras da colheita de variedades de mandiocas selecionadas para a produção de raiz possuem cons

Characterization of cassava (Manihot sculenta crantz) aerial parts for ruminant feeding / Caracterização da parte aérea de mandioca para alimentação animal

The objective of this research was to characterize the chemical composition and digestibility of aerial part and cuttings of four cassava cultivars grown in five different levels of soil fertilization and three plant densities, in order to use the residues for ruminant feeding. The experiment was conducted using a randomized complete block design, in strip-splitplot scheme, with four replicates. The plots were composed of IAC14, IAC15, IAC90, and Cascuda cultivars; the subplots consisted of five fertilization categories, one fertilization with poultry litter (3000 kg.ha-1 ) and four chemical fertilization rates (0, 150, 450, 900 kg.ha-1 ) with NPK 4-20-20; and with the sub-subplots consisted of three plant densities (7,500, 12,500, 17,500 plants.ha-1 ). During the experiment, aerial part and cuttings were sampled for chemical and in vitro digestibility analyzes. There were differences between the varieties of cassava and fertilization rates on fiber fractions, chemical composition, in vitro digestibility, total digestible nutrients (TDN), and dry matter yield (kg / ha) of aerial part (P < 0.05) and cuttings (P < 0.05). Cassava aerial part and cuttings have considerable amounts of nutrients that can be used for ruminant feeding on small farms and for animal categories with low nutritional requirements. From all treatments, variety IAC15, with chemical fertilization of 900 kg.ha-1, as well as fertilization with poultry litter, with the highest plant density, were those with better nutritional composition and greater amount of available nutrients. (AU)

O objetivo desta pesquisa foi quantificar e estudar a composição química e a digestibilidade da parte aérea e da manivamãe de quatro cultivares de mandioca, cultivadas sob cinco diferentes níveis de adubações e três densidades de plantio, com a finalidade de utilização destes coprodutos na alimentação de ruminantes. O experimento foi realizado no Pólo Regional de Desenvolvimento do Médio Paranapanema utilizando o delineamento experimental em blocos casualizados, em esquema de parcelas sub-subdivididas, com quatro repetições. As parcelas foram compostas pelas cultivares IAC 14, IAC 15, IAC 90 e Cascuda; as subparcelas compostas por doses de 4-20-20 (0, 150, 450, 900 kg/ha) ou adubação com cama de frango (3.000 kg/ha de cama de frango) e as sub-subparcelas compostas por densidades de plantio de 7.500, 12.500, 17.500 plantas/ha. Foram feitas amostragens de parte aérea e maniva-mãe e realizadas análises químicas para determinação dos nutrientes e digestibilidade in vitro. Houve diferença entre as variedades de mandioca e níveis de fertilização sobre as frações da fibra; composição química; digestibilidade in vitro; nutrientes digestíveis totais (NDT), e rendimento de matéria seca (kg/ha) da parte aérea (P < 0,05) e da maniva-mãe (P < 0,05). A parte aérea e a maniva-mãe oriundas das sobras da colheita de variedades de mandiocas selecionadas para a produção de raiz possuem cons

Semen variables and sperm membrane protein profile of Saanen bucks (Capra hircus) in dry and rainy seasons of the northeastern Brazil (3°S).

The Saanen is a highly productive breed, and for this reason, it has been raised in Brazil, but mostly under climate conditions completely different from where the breed originated. The objective of this study was to investigate variations in semen parameters and sperm membrane proteins from Saanen bucks (n = 7) raised in Northeastern Brazil, during dry season (September, October, and November) and rainy season (March, April, and May). We showed that during the dry season, sperm motility, concentration, and the percentage of normal sperm decreased as compared to the rainy season. Rectal temperatures of bucks had no significant (p > 0.05) variations during the dry and rainy seasons. However, temperatures of left and right skin testis were higher (p < 0.05) during the dry as compared to the rainy season. Expression of three proteins (lysine-specific demethylase 5D, adenosine triphosphate (ATP) synthase subunit d, and radial spoke head protein 9 homolog) in sperm membrane were more intense in rainy season and only one protein (cytosol aminopeptidase) had greater expression in the dry season of the year. Our results show that mechanisms of testicular thermoregulation of Saanen bucks did not prevent a decrease in seminal parameters during the dry season. This deterioration may be related to reduced expression of proteins associated with important functions in sperm membrane.

Improvement in the outcome of invasive fusariosis in the last decade.

Invasive fusariosis (IF) has been associated with a poor prognosis. Although recent series have reported improved outcomes, the definition of optimal treatments remains controversial. The objective of this study was to evaluate changes in the outcome of IF. We retrospectively analysed 233 cases of IF from 11 countries, comparing demographics, clinical findings, treatment and outcome in two periods: 1985-2000 (period 1) and 2001-2011 (period 2). Most patients (92%) had haematological disease. Primary treatment with deoxycholate amphotericin B was more frequent in period 1 (63% vs. 30%, p <0.001), whereas voriconazole (32% vs. 2%, p <0.001) and combination therapies (18% vs. 1%, p <0.001) were more frequent in period 2. The 90-day probabilities of survival in periods 1 and 2 were 22% and 43%, respectively (p <0.001). In period 2, the 90-day probabilities of survival were 60% with voriconazole, 53% with a lipid formulation of amphotericin B, and 28% with deoxycholate amphotericin B (p 0.04). Variables associated with poor prognosis (death 90 days after the diagnosis of fusariosis) by multivariable analysis were: receipt of corticosteroids (hazard ratio (HR) 2.11, 95% CI 1.18-3.76, p 0.01), neutropenia at end of treatment (HR 2.70, 95% CI 1.57-4.65, p <0.001), and receipt of deoxycholate amphotericin B (HR 1.83, 95% CI 1.06-3.16, p 0.03). Treatment practices have changed over the last decade, with an increased use of voriconazole and combination therapies. There has been a 21% increase in survival rate in the last decade.

Survival, growth, and hormone production in vitro from preantral follicles of crossbred female goats (Saanen × Anglo-Nubian)

The present study aimed to evaluate the survival, growth, antrum formation, oocyte extrusion, and hormone production during in vitro culture of caprine preantral follicles isolated from pure-breed (Saanen) or crossbreed (F1 generation: ½ Saanen + ½ Anglo-Nubian) goats. Secondary follicles (diameter: 150-250 µm) from Saanen or crossbreed (Saanen × Anglo-Nubian) goats were isolated from the ovarian cortex by microdissection and cultured in vitro for 18 days in α-modified minimum essential medium (α-MEM+) supplemented with vascular endothelial growth factor and increasing concentrations of follicle-stimulating hormone. Every six days follicular morphology, growth, antrum formation, and follicular extrusion were evaluated. In addition, on days 2, 6, 12, and 18 of culture the medium samples were collected and stored at -20°C for further measurement of estradiol and progesterone. The follicular survival, antrum formation, and oocyte extrusion were analyzed by the Chi square test. Follicular diameter and hormone assays were compared using the Kruskal-Wallis test. Survival rates, growth, antral follicle formation, and oocyte extrusion of preantral follicles cultured in vitro were similar between the different genetic groups. The production of estradiol and progesterone indicated the maintenance of cell viability throughout the culture. In conclusion, preantral follicles from pure-breed or crossbreed goats can be used with the same efficiency for in vitro culture of isolated caprine preantral follicles.

Survival, growth, and hormone production in vitro from preantral follicles of crossbred female goats (Saanen × Anglo-Nubian)

The present study aimed to evaluate the survival, growth, antrum formation, oocyte extrusion, and hormone production during in vitro culture of caprine preantral follicles isolated from pure-breed (Saanen) or crossbreed (F1 generation: ½ Saanen + ½ Anglo-Nubian) goats. Secondary follicles (diameter: 150-250 µm) from Saanen or crossbreed (Saanen × Anglo-Nubian) goats were isolated from the ovarian cortex by microdissection and cultured in vitro for 18 days in α-modified minimum essential medium (α-MEM+) supplemented with vascular endothelial growth factor and increasing concentrations of follicle-stimulating hormone. Every six days follicular morphology, growth, antrum formation, and follicular extrusion were evaluated. In addition, on days 2, 6, 12, and 18 of culture the medium samples were collected and stored at -20°C for further measurement of estradiol and progesterone. The follicular survival, antrum formation, and oocyte extrusion were analyzed by the Chi square test. Follicular diameter and hormone assays were compared using the Kruskal-Wallis test. Survival rates, growth, antral follicle formation, and oocyte extrusion of preantral follicles cultured in vitro were similar between the different genetic groups. The production of estradiol and progesterone indicated the maintenance of cell viability throughout the culture. In conclusion, preantral follicles from pure-breed or crossbreed goats can be used with the same efficiency for in vitro culture of isolated caprine preantral follicles.(AU)

Resistant gram-negative infections in the outpatient setting in Latin America.

Latin America has a high rate of community-associated infections caused by multidrug-resistant Enterobacteriaceae relative to other world regions. A review of the literature over the last 10 years indicates that urinary tract infections (UTIs) by Escherichia coli, and intra-abdominal infections (IAIs) by E. coli and Klebsiella pneumoniae, were characterized by high rates of resistance to trimethoprim/sulfamethoxazole, quinolones, and second-generation cephalosporins, and by low levels of resistance to aminoglycosides, nitrofurantoin, and fosfomycin. In addition, preliminary data indicate an increase in IAIs by Enterobacteriaceae producing extended-spectrum ß-lactamases, with reduced susceptibilities to third- and fourth-generation cephalosporins. Primary-care physicians in Latin America should recognize the public health threat associated with UTIs and IAIs by resistant Gram-negative bacteria. As the number of therapeutic options become limited, we recommend that antimicrobial prescribing be guided by infection severity, established patient risk factors for multidrug-resistant infections, acquaintance with local antimicrobial susceptibility data, and culture collection.

Gestational exposure to yellow fever vaccine at different developmental stages induces behavioral alterations in the progeny.

The most effective method to prevent yellow fever and control the disease is a vaccine made with attenuated live virus. Due to the neurological tropism of the virus, preventive vaccination is not recommended for infants under 6 months and for pregnant women. However there is a paucity of data regarding the safety for pregnant women and there are no experimental studies investigating adverse effects to the offspring after maternal exposure to the vaccine. This study aimed to investigate, in mice, the effects of maternal exposure to the yellow fever vaccine at three different gestational ages on the physical and behavioral development of the offspring. Pregnant Swiss mice received a single subcutaneous injection of water for injection (control groups) or 2 log Plaque Forming Units (vaccine-treated groups) of the yellow fever vaccine on gestational days (GD) 5, 10 or 15. Neither maternal signs of toxicity nor alterations in physical development and reflex ontogeny of the offspring were observed in any of the groups. Data from behavioral evaluation indicated that yellow fever vaccine exposure induced motor hypoactivity in 22-day-old females independent of the day of exposure; and in 60-day-old male and female pups exposed at GD 10. Moreover, 22-day-old females also presented with a deficit in habituation memory. Altogether, these results indicate that in utero exposure to the yellow fever vaccine may induce behavioral alterations in the pups that may persist to adulthood in the absence of observed maternal toxicity or disruption of physical development milestones or reflex ontogeny.

Alterações histopatológicas da glândula mamária e qualidade do leite de cabras naturalmente infectadas com o CAEV / Histopathological changes in the mammary gland and milk quality of goats naturally infected with CAEV

Avaliou-se a influência do vírus da CAE nas características físico-químicas de amostras de leite de 54 cabras, sem predileção racial, distribuindo-as em dois grupos: cabras positivas e negativas para o teste de imunodifusão em gel de agarose. As amostras de leite foram submetidas à análise ultrassônica para obtenção de parâmetros físico-químicos - gordura, extrato seco, proteínas, lactose e densidade; realização de microbiologia - bactérias mesófilas (UCF/mL). Foram coletadas amostras de tecido mamário para exame histopatológico e imunohistoquímica. Não houve diferença significativa das características avaliadas entre os dois grupos; no microbiológico, não houve relação direta da presença de mesófilas associada à infecção pelo CAEV. Na histopatologia, observaram-se áreas com infiltração celular de monócitos, polimorfonucleares, plasmócitos, fibrose, ausência de morfologia normal do parênquima mamário, denotando processo inflamatório crônico; e foi confirmada a presença do vírus na glândula pela imunohistoquímica. Os resultados não mostraram relação direta da incidência da CAE como fator negativo no desenvolvimento do rebanho.

Aiming to evaluate the influence of CAE viruses in the chemical and physical characteristics of milk, the samples were collected from 54 goats, without racial predilection, these were divided into two groups: goats positive and negative according results of test Agarose Gel Immunodiffusion. Milk samples were ultrasonic analyzed to obtain physicochemical parameters (fat, solids, protein, lactose and density); performance microbiology (mesophilic bacteria - CFU/mL) and mammary gland samples were collected for evaluation histopathology and immunohistochemistry. The results of physical-chemical analysis showed no significant difference between the milk samples of two groups. In the microbiological analysis showed the presence of aerobic mesophilic bacteria, but this change is not associated with the presence of CAEV infection. On histopathology, there were areas with infiltration of mononuclear-leukocyte and polymorph nuclear, plasma cells, fibrosis and absence of normal morphology of the mammary tissue, indicating a chronic inflammatory process; and confirmed the presence of virus, in the gland, by immunohistochemistry. The results showed no direct relationship between incidence of CAE in the herd as a negative factor for its development, however it is known that the disease in its chronic nature, causes reduction in the productivity of the herd.

Antibody response to the non-adjuvanted and adjuvanted influenza A H1N1/09 monovalent vaccines in renal transplant recipients.

BACKGROUND: The 2009 pandemic influenza A (H1N1) virus spread rapidly throughout Brazil. Non-adjuvanted and the adjuvanted influenza A H1N1/09 monovalent vaccine were recommended as a single dose to persons at risk including renal transplant recipients (RTR). We analyzed the safety and the immune response of 2 influenza A H1N1/09 monovalent vaccines in RTR, and identified factors influencing the immune response. METHODS: A total of 78 RTR received a single dose of either influenza A H1N1 2009 monovalent AS03-adjuvanted vaccine or a non-adjuvanted vaccine, and 58 healthy controls received a single dose of non-adjuvanted vaccine. Antibody responses to influenza A H1N1 were measured by hemagglutination inhibition assay and were compared between groups on the day of vaccination and 21-30 days thereafter, using geometric mean titer (GMT), and seroprotection (SP) and seroconversion (SC) rates. RESULTS: Among RTR, after adjuvanted and non-adjuvanted H1N1 vaccination, the SP rate increased from 16.7% to 61.7% (P < 0.001) and to 50% (P < 0.001), and SC rates were 61.7% and 50%, respectively. For healthy controls, SP rate increased from 25.8% to 89.7% (P < 0.001), and SC rate was 87.9% after vaccination. Pre-vaccination GMT for the adjuvanted and non-adjuvanted RTR vaccine groups and healthy controls was 9.7 (95% confidence interval [CI] 7.3-13.1), 8.9 (95% CI 5.4-14.7), and 12.5 (95% CI8.7-18.2), and significantly increased to 49.8 (95% CI 31.3-79.4, P < 0.001), 43.2 (95% CI 16.3-114.4, P < 0.001), and 323.8 (95% CI 213.9-490.2, P < 0.001), respectively. Deceased-donor type transplant significantly reduced SP (odds ratio [OR] = 4.62, 95% CI 1.36-15.69, P = 0.014) and SC (OR = 6.29, 95% CI 1.89-20.98, P = 0.003) rates, and younger age positively affected SP (OR = 0.11; 95% CI 0.03-0.04, P = 0.001). Adverse events were mild, and renal function showed no change post vaccination. CONCLUSION: RTR vaccinated with either an adjuvanted or non-adjuvanted monovalent influenza vaccine presented poor response compared with healthy controls. Post-vaccination adverse events were mild, and no rejection episode or renal dysfunction was observed.

Impacto do estresse térmico na reprodução da fêmea bovina / Heat stress impact on reproduction of bovine female

Com o objetivo de melhorar os índices de produtividade de seus rebanhos, muitos criadores de regiões tropicais têm optado pela aquisição de animais especializados oriundos de regiões de clima temperado com condições climáticas bem diferenciadas das dos trópicos. Devido às altas temperaturas tropicais, estes animais estão constantemente susceptíveis ao estresse térmico, uma vez que não são dotados de mecanismos anatomo-fisiológicos necessários para manter a homeotermia, resultando em prejuízos para a produção e reprodução animal. Portanto, esta revisão tem por objetivo discorrer sobre os efeitos negativos do estresse térmico sobre a reprodução, enfocando aspectos relacionados à atividade ovariana e à mortalidade embrionária.

Aiming to improve the productivity rates of their herds, many farmers in tropical regions have opted for the acquisition of specialized animals come from temperate regions with weather conditions so different from those found in the tropics. Due to high tropical temperatures, these animals are constantly susceptible to heat stress; because they do not have anatomical and physiological mechanisms to maintain efficiently its homeostasis, resulting in prejudices to production and reproduction performances. This review aims to discuss the adverse effects of heat stress on female reproduction, emphasizing some aspects involved in ovarian activity and embryo mortality.

Impacto do estresse térmico na reprodução da fêmea bovina / Heat stress impact on reproduction of bovine female

Com o objetivo de melhorar os índices de produtividade de seus rebanhos, muitos criadores de regiões tropicais têm optado pela aquisição de animais especializados oriundos de regiões de clima temperado com condições climáticas bem diferenciadas das dos trópicos. Devido às altas temperaturas tropicais, estes animais estão constantemente susceptíveis ao estresse térmico, uma vez que não são dotados de mecanismos anatomo-fisiológicos necessários para manter a homeotermia, resultando em prejuízos para a produção e reprodução animal. Portanto, esta revisão tem por objetivo discorrer sobre os efeitos negativos do estresse térmico sobre a reprodução, enfocando aspectos relacionados à atividade ovariana e à mortalidade embrionária.(AU)

Aiming to improve the productivity rates of their herds, many farmers in tropical regions have opted for the acquisition of specialized animals come from temperate regions with weather conditions so different from those found in the tropics. Due to high tropical temperatures, these animals are constantly susceptible to heat stress; because they do not have anatomical and physiological mechanisms to maintain efficiently its homeostasis, resulting in prejudices to production and reproduction performances. This review aims to discuss the adverse effects of heat stress on female reproduction, emphasizing some aspects involved in ovarian activity and embryo mortality.(AU)

Alterações histopatológicas da glândula mamária e qualidade do leite de cabras naturalmente infectadas com o CAEV / Histopathological changes in the mammary gland and milk quality of goats naturally infected with CAEV

Avaliou-se a influência do vírus da CAE nas características físico-químicas de amostras de leite de 54 cabras, sem predileção racial, distribuindo-as em dois grupos: cabras positivas e negativas para o teste de imunodifusão em gel de agarose. As amostras de leite foram submetidas à análise ultrassônica para obtenção de parâmetros físico-químicos - gordura, extrato seco, proteínas, lactose e densidade; realização de microbiologia - bactérias mesófilas (UCF/mL). Foram coletadas amostras de tecido mamário para exame histopatológico e imunohistoquímica. Não houve diferença significativa das características avaliadas entre os dois grupos; no microbiológico, não houve relação direta da presença de mesófilas associada à infecção pelo CAEV. Na histopatologia, observaram-se áreas com infiltração celular de monócitos, polimorfonucleares, plasmócitos, fibrose, ausência de morfologia normal do parênquima mamário, denotando processo inflamatório crônico; e foi confirmada a presença do vírus na glândula pela imunohistoquímica. Os resultados não mostraram relação direta da incidência da CAE como fator negativo no desenvolvimento do rebanho.(AU)

Aiming to evaluate the influence of CAE viruses in the chemical and physical characteristics of milk, the samples were collected from 54 goats, without racial predilection, these were divided into two groups: goats positive and negative according results of test Agarose Gel Immunodiffusion. Milk samples were ultrasonic analyzed to obtain physicochemical parameters (fat, solids, protein, lactose and density); performance microbiology (mesophilic bacteria - CFU/mL) and mammary gland samples were collected for evaluation histopathology and immunohistochemistry. The results of physical-chemical analysis showed no significant difference between the milk samples of two groups. In the microbiological analysis showed the presence of aerobic mesophilic bacteria, but this change is not associated with the presence of CAEV infection. On histopathology, there were areas with infiltration of mononuclear-leukocyte and polymorph nuclear, plasma cells, fibrosis and absence of normal morphology of the mammary tissue, indicating a chronic inflammatory process; and confirmed the presence of virus, in the gland, by immunohistochemistry. The results showed no direct relationship between incidence of CAE in the herd as a negative factor for its development, however it is known that the disease in its chronic nature, causes reduction in the productivity of the herd.(AU)

Efeitos da fonte de enxofre sobre a população de protozoários e degradabilidade no rúmen / Effects of sulfur source on protozoa population and ruminal degradability

Estudaram-se os efeitos de fontes de enxofre na dieta sobre os parâmetros ruminais de bovinos Nelore, utilizando-se oito novilhos canulados no rúmen e submetidos a quatro tratamentos, segundo a fonte de enxofre. Os animais do grupo-controle não receberam suplementação de enxofre. Os dos outros três grupos receberam flor de enxofre ou metionina ou carboquelatado, como suplementação. Os animais foram arraçoados com dieta total, utilizando cana-de-açúcar picada como volumoso. O período experimental foi de 76 dias, dividido em quatro subperíodos de 19 dias, dos quais 14 eram para adaptação à dieta e cinco para as colheitas. Não foram encontradas diferenças entre as fontes de enxofre na degradabilidade da matéria seca, proteína bruta e fibras em detergente ácido e neutro e no pH ruminal. O tratamento com carboquelatado resultou em maior número de protozoários ciliados e o tratamento metionina em menor contagem. O carboquelatado pode ser uma boa alternativa para a suplementação de enxofre para bovinos em confinamento.

This research was carried out at FZEA/USP to compare the effects of different sulphur sources in diets of Nellore cattle on ruminal parameters. Eight steers were rumen cannulated and submitted to four treatments, using the following sulphur sources: control (no supplement), elemental sulphur, methionine, and carboquelated. Animals were fed a total ration using sugarcane as the roughage. The proportion roughage:concentrate was 40:60. The experimental period lasted 76 days, divided in four 19-days sub-periods. The first 14 days of each sub-period were used to adapt the animals to the diet and the last five days to collect the samples. During the sample period, ruminal liquid was sampled to protozoa count and pH determination. Also, nylon bags were incubated in rumen to determinate the degradability of dietary dry matter, crude protein, and acid and neutral detergent fiber. Treatments did not affect dietary dry matter, crude protein, acid and neutral detergent fiber degradability, and the ruminal pH. However, carboquelated provided the higher total amount of ciliate protozoa and methionine provided the lowest values. Carboquelated can be a good alternative to supplement sulphur to bovines in a feedlot.

Efeitos da fonte de enxofre sobre a população de protozoários e degradabilidade no rúmen / Effects of sulfur source on protozoa population and ruminal degradability

Estudaram-se os efeitos de fontes de enxofre na dieta sobre os parâmetros ruminais de bovinos Nelore, utilizando-se oito novilhos canulados no rúmen e submetidos a quatro tratamentos, segundo a fonte de enxofre. Os animais do grupo-controle não receberam suplementação de enxofre. Os dos outros três grupos receberam flor de enxofre ou metionina ou carboquelatado, como suplementação. Os animais foram arraçoados com dieta total, utilizando cana-de-açúcar picada como volumoso. O período experimental foi de 76 dias, dividido em quatro subperíodos de 19 dias, dos quais 14 eram para adaptação à dieta e cinco para as colheitas. Não foram encontradas diferenças entre as fontes de enxofre na degradabilidade da matéria seca, proteína bruta e fibras em detergente ácido e neutro e no pH ruminal. O tratamento com carboquelatado resultou em maior número de protozoários ciliados e o tratamento metionina em menor contagem. O carboquelatado pode ser uma boa alternativa para a suplementação de enxofre para bovinos em confinamento.(AU)

This research was carried out at FZEA/USP to compare the effects of different sulphur sources in diets of Nellore cattle on ruminal parameters. Eight steers were rumen cannulated and submitted to four treatments, using the following sulphur sources: control (no supplement), elemental sulphur, methionine, and carboquelated. Animals were fed a total ration using sugarcane as the roughage. The proportion roughage:concentrate was 40:60. The experimental period lasted 76 days, divided in four 19-days sub-periods. The first 14 days of each sub-period were used to adapt the animals to the diet and the last five days to collect the samples. During the sample period, ruminal liquid was sampled to protozoa count and pH determination. Also, nylon bags were incubated in rumen to determinate the degradability of dietary dry matter, crude protein, and acid and neutral detergent fiber. Treatments did not affect dietary dry matter, crude protein, acid and neutral detergent fiber degradability, and the ruminal pH. However, carboquelated provided the higher total amount of ciliate protozoa and methionine provided the lowest values. Carboquelated can be a good alternative to supplement sulphur to bovines in a feedlot.(AU)

Influência do balanco cátion-aniônico da dieta no rúmen e no desempenho de ovinos / Influence of dietary cation-anion balance on rumen and performance variables in sheep

Estudou-se o efeito do balanço cátion-aniônico da dieta (BCAD) no desempenho animal e na fermentação ruminal, utilizando-se 25 carneiros machos, da raça Santa Inês, durante 75 dias. O delineamento experimental foi em blocos ao acaso, com cinco tratamentos. Para a manipulação do BCAD, foram adicionados sulfato de amônio e bicarbonato de sódio, obtendo-se os seguintes tratamentos: -160, -40, 140, 250 e 500 mEq/kg de matéria seca. O aumento do BCAD resultou em aumentos da ingestão de matéria seca, do ganho diário, da eficiência alimentar e do pH ruminal (P<0,05). O perfil de ácidos graxos ruminais não foi afetado pelo BCAD.(AU)

The effect of dietary cation-anion balance (DCAB) on dry matter intake, daily weight gain, feed conversion efficiency, rumen pH and rumen volatile fatty acid profile in Santa Ines sheep was studied. Dietary treatments were formulated combining sodium sulphate and sodium bicarbonate in appropriate ratios to achieve DCAB balance of –160, -40, 140, 250 and 500 mEq/kg dry matter. Twenty five male lambs were assigned to the five treatments in a randomized block design for a 75-day feeding trial. Increasing DCAB was associated with increasing voluntary dry matter intake, daily weight gain, feed conversion efficiency and rumen pH (P<0.05); but DCAB did not affect the volatile fatty acid profile in the rumen.(AU)