Impact of acute hypoxic exposure on neuromuscular and hemodynamic responses during step intensity dynamic constant external resistance leg extension exercise.

OBJECTIVES: This study examined the effects of acute normoxic and hypoxic exposure on neuromuscular and hemodynamic physiological responses performed during dynamic step muscle actions. METHODS: Thirteen recreationally active men (mean ± SD age: 21.2 ± 2.9 yrs) performed dynamic leg extensions unilaterally under Normoxic (FiO2 = 21 %) and Hypoxic (FiO2 = 13 %) conditions in a randomized order at 20 %, 40 %, 60 %, 80 %, and 100 % of their maximal strength. Electromyographic (EMG) amplitude, EMG frequency, (Oxygenated and Deoxygenated hemoglobin; OxyHb, DeoxyHb), Total hemoglobin (TotalHb), and skeletal muscle tissue oxygenation status (StO2) were measured from the vastus lateralis during all contractions. RESULTS: There were no detectable differences in the neuromuscular responses between normoxia and hypoxia for EMG amplitude (p = 0.37-0.74) and frequency (p = 0.17-0.83). For EMG amplitude there were general increases with intensity (p < 0.01-0.03). EMG frequency remained similar from 20% to 80% and then increased at 100 % effort (p = 0.02). There was no significant difference in patterns of responses for OxyHb (p = 0.870) and TotalHb (p = 0.200) between normoxia and hypoxia. StO2 (p = 0.028) decreased and DeoxyHb (p = 0.006) increased under hypoxia compared to normoxia during dynamic step muscle actions performed in a randomized order. CONCLUSION: Unlike fatigue, acute hypoxemia in an unfatigued state does not impact the localized neuromuscular responses, but minimally impacts the hemodynamic responses.

The impact of cold, hypoxia, and physical exertion on pistol accuracy and tactical performance.

The purpose of this study was to examine the impact of independent cold and combined cold and hypoxic exposures on operational-specific task performance including pistol marksmanship, pistol magazine reload ability, and subjective and objective thermal indices before and after a whole-body physical exertional task. Twelve participants were exposed to Thermoneutral Normoxic (24 °C; FiO2 21%), Cold Normoxic (10 °C; FiO2 21%), and Cold Hypoxic (10 °C; FiO2 14%) conditions for 30min before performing pistol marksmanship at distances of 6.40 and 13.72m and a pistol magazine reload task before and after 3 sets of sandbag deadlifts at 50% body mass. Thermal perception and hand temperatures were collected before and after the physical exertion task. There were no significant differences in Pistol Accuracy performance at distances of 6.40 and 13.72m due to physical exertion, cold, or hypoxia. Following physical exertion, Pistol Accuracy was similar between Thermoneutral and Cold Normoxic conditions but lead to 17% and a 10% reduction in performance during the Cold Hypoxic condition, compared to Thermoneutral and Cold Normoxic conditions. There was no change in Pistol Accuracy for the Thermoneutral Normoxic condition. The pistol magazine reload task was not impacted by physical exertion, but there was a reduction in performance in Cold Normoxic 21% (4.04s) and Cold Hypoxic 16% (3.08s) conditions. Physical exertion did not impact hand temperature but did increase thermal perception scores for all conditions. These findings indicate that cold exposure reduced both tactical dexterity and pistol marksmanship, however, physical exertion may offset these deficits via an increase in thermal perception. Additionally, hypoxemia was the primary mediator of marksmanship performance in cold hypoxic environments following an acute bout of physical exertion. Thus, in cold mountainous environments, marksmen should be aware of their elevation and utilize brief episodes of physical activity to enhance their thermal state when marksmanship is a priority for operational success.

Neuromuscular and Muscle Tissue Hemodynamic Responses When Exposed to Normobaric Hypoxia during Lower-Body Fatiguing Muscle Actions.

OBJECTIVES: This study examined effects of acute hypoxia on the neuromuscular responses (electromyographic (EMG) amplitude and EMG frequency) and localized muscle tissue oxygenated hemoglobin (oxygenated hemoglobin (OxyHb), deoxygenated hemoglobin (DeoxyHb), total hemoglobin (TotalHb), and muscle tissue oxygenation saturation (StO2) during the process of fatigue. METHODS: Fifteen male participants (21.4±2.8yr) performed leg extension repetitions to failure at 70% 1-repetition maximum until volitional exhaustion under Normoxic (FiO2:21%) and Hypoxic (FiO2:12.9%) conditions. Electromyographic amplitude, EMG frequency, OxyHb, DeoxyHb, TotalHb, and StO2 were measured from the vastus lateralis at Initial, 20, 40, 60, 80, and 100% of the repetitions to failure. RESULTS: There was no significant difference in the patterns of responses for EMG amplitude, OxyHb, or DeoxyHb between Normoxia and Hypoxia. For EMG frequency, Hypoxia was greater than Normoxia and decreased with fatigue. TotalHb and StO2 were greater under Normoxia compared to Hypoxia. The patterns of responses for EMG amplitude, DeoxyHb, and TotalHb increased throughout the repetitions to failure. OxyHb and StO2 exhibited decreases throughout the repetitions to failure for Normoxic and Hypoxic conditions. CONCLUSION: The EMG and oxygenation measurements non-invasively suggest a sympathoexcitatory response (indicated by EMG frequency) and provided complimentary information regarding the process of fatigue in normoxic and hypoxic states.

Moderate and Severe Acute Normobaric Hypoxia and the 3-Repetition Deadlift, Hand-Release Push-Up, and Leg Tuck Events From the Army Combat Fitness Test.

INTRODUCTION: The newly implemented Army Combat Fitness Test (ACFT) of the U.S. Army seeks to revolutionize the Army's fitness culture and reduce the rate of preventable injuries among soldiers. The initial rollout of the ACFT has been met with several challenges, including a gender-neutral scoring system. The ACFT has undergone several revisions to adapt to the present state of U.S. Army physical fitness; however, the test faces several more obstacles as more data become available. The ACFT was designed to measure combat readiness, a useful tool for units facing deployment or a change in duty station to a high-altitude environment. Reduced oxygen availability (hypoxia) at high altitude influences many physiological functions associated with physical fitness, such that there is an increased demand for oxygen in exercising muscle. Therefore, the purpose was to investigate the effects of normoxic and two levels of hypoxia exposure (moderate and severe; fraction of inspired oxygen [FiO2]: 16.0% and 14.3%) during the 3-repetition deadlift (MDL), hand-release push-up (HRP), and leg tuck (LTK) events of the ACFT. MATERIALS AND METHODS: Fourteen recreationally active men (n = 10) and women (n = 4) soldier analogs (27.36 ± 1.12 years, height 1.71 ± 2.79 m, weight 80.60 ± 4.24 kg) completed the MDL, HRP, and LTK at normoxia and acute normobaric moderate (MH; FiO2 16%) and severe (SH; FiO2 14.3%) hypoxic exposure. Scores and performance were recorded for each event, and heart rate (HR) and total body oxygen saturation (SpO2) were monitored throughout. Repeated-measures analysis of variance (ANOVA) was used to assess differences in modified ACFT scores, performance, HR, and SpO2 among hypoxic conditions, with follow-up one-way ANOVA and paired t-test when appropriate. RESULTS: Total body oxygen saturation was decreased at MH and SH conditions compared to normoxia but did not vary between ACFT events. Heart rate was not influenced by altitude but did increase in response to exercise. Scores of the modified total and individual ACFT events were not different between normoxia, MH, and SH. There was also no difference in performance based on the amount of weight lifted during the MDL and number of repetitions of the HRP and LTK events in response to hypoxic exposure. CONCLUSIONS: Performance and scores of the modified ACFT were not influenced by acute normobaric MH and SH exposure compared to normoxia. Further investigations should examine the full testing battery of the ACFT to provide a comprehensive analysis and potential evidence for such differences.

Changes in Neuromuscular Response Patterns After 4 Weeks of Leg Press Training During Isokinetic Leg Extensions.

ABSTRACT: Salmon, OF, Housh, TJ, Hill, EC, Keller, JL, Anders, JPV, Johnson, GO, Schmidt, RJ, and Smith, CM. Changes in neuromuscular response patterns after 4 weeks of leg press training during isokinetic leg extensions. J Strength Cond Res 37(7): e405-e412, 2023-The purpose of this study was to identify velocity-specific changes in electromyographic root mean square (EMG RMS), EMG frequency (EMG MPF), mechanomyographic RMS (MMG RMS), and MMG MPF during maximal unilateral isokinetic muscle actions performed at 60° and 240°·s -1 velocities within the right and left vastus lateralis (VL) after 4 weeks of dynamic constant external resistance (DCER) bilateral leg press training. Twelve resistance-trained men (age: mean ± SD = 21.4 ± 3.6 years) visited the laboratory 3d·wk -1 to perform resistance training consisting of 3 sets of 10 DCER leg presses. Four, three-way analysis of variance were performed to evaluate changes in neuromuscular responses (EMG RMS, EMG MPF, MMG RMS, and MMG MPF) from the right and left VL during 1 single-leg maximal isokinetic leg extension performed at 60° and 240°·s -1 before and after 4 weeks of DCER leg press training ( p < 0.05). The results indicated a 36% increase in EMG RMS for the right leg, as well as a 23% increase in MMG RMS and 10% decrease in MMG MPF after training, collapsed across velocity and leg. In addition, EMG RMS was 65% greater in the right leg than the left leg following training, whereas EMG MPF was 11% greater for the left leg than the right leg throughout training. Thus, 4 weeks of DCER leg press training provides sufficient stimuli to alter the neuromuscular activation process of the VL but not velocity-specific neuromuscular adaptations in trained males.

Influences of Vitamin D and Iron Status on Skeletal Muscle Health: A Narrative Review.

There is conflicting evidence of the roles vitamin D and iron have in isolation and combined in relation to muscle health. The purpose of this narrative review was to examine the current literature on the roles that vitamin D and iron have on skeletal muscle mass, strength, and function and how these nutrients are associated with skeletal muscle health in specific populations. Secondary purposes include exploring if low vitamin D and iron status are interrelated with skeletal muscle health and chronic inflammation and reviewing the influence of animal-source foods rich in these nutrients on health and performance. PubMed, Scopus, SPORT Discus, EMBAE, MEDLINE, and Google Scholar databases were searched to determine eligible studies. There was a positive effect of vitamin D on muscle mass, particularly in older adults. There was a positive effect of iron on aerobic and anaerobic performance. Studies reported mixed results for both vitamin D and iron on muscle strength and function. While vitamin D and iron deficiency commonly occur in combination, few studies examined effects on skeletal muscle health and inflammation. Isolated nutrients such as iron and vitamin D may have positive outcomes; however, nutrients within food sources may be most effective in improving skeletal muscle health.

Cognitive Function is Unaffected during Acute Hypoxic Exposure but was Improved Following Exercise.

To investigate the effects of two levels of acute hypoxic exposure and exercise compared to normoxia on the Stroop color word test. A total of 14 (4 females and 10 males) active participants with a self-reported (mean±SEM) 8.54±1.44 h/week of physical activity, performed a 3-repetition maximum hex/trap bar deadlift, Hand-Release Push-Up, and Leg Tuck events from the Army Combat Fitness Test at normoxia and normobaric hypoxia of fraction of inspired oxygen (FiO2) of 16% and 14.3%. The Stroop color-word test was administered on a touch screen device before and after the exercise battery, where participants were given congruent (word and ink color matching) and incongruent (non-matching) prompts. Peripheral oxygen saturation (SpO2) and heart rate were recorded at pre- and post-exercise. Variables obtained from the Stroop color word test were not influenced as a result of acute hypoxic exposure but did improve after an exercise battery. Peripheral oxygen saturation was greater during normoxia compared to acute hypoxic exposure which indicated a systemic change in oxygenation. The results of the present study indicated that the Stroop color-word test is not influenced by an FiO2 16% or 14.3%, however, exercise did improve Stroop score and response time.

Neuromuscular responses at acute moderate and severe hypoxic exposure during fatiguing exercise of the biceps brachii.

PURPOSE: The present study examined acute normobaric hypoxic exposure on the number of repetitions to failure, electromyographic (EMG) repetition duration (Time), EMG root mean square (RMS) and EMG mean power frequency (MPF) during biceps brachii (BB) dynamic constant external resistance (DCER) exercise. METHODS: Thirteen subjects performed two sets of fatiguing DCER arm curl repetitions to failure at 70% of their one repetition maximum under normoxic (NH), moderate hypoxia FiO2 = 15% (MH) and severe hypoxia FiO2 = 13% (SH). Electromyography of the BB was analyzed for EMG Time, EMG RMS, and EMG MPF. Repetitions were selected as 25%, 50%, 75%, and 100% of total repetitions (%Fail) completed. Pulse oximetry (SpO2) was measured pre-and post-fatigue. RESULTS: There was no significant three-way (Condition x Set x %Fail) or two-way (Condition x Set) interaction for any variable. The number of repetitions to failure significantly decreased from (mean ± SEM) 18.2 ± 1.4 to 9.5 ± 1.0 with each Set. In addition, EMG Time increased (25% < 50%<75% < 100%), EMG RMS decreased (50% > 75%>100%), and EMG MPF decreased (75% > 100%) as a result of fatiguing exercise. SpO2 was lower during MH (Δ5.3%) and SH (Δ9.2%) compared to NH and as a result of fatiguing exercise increased only in MH (Δ2.1%) and SH (Δ5.7%). CONCLUSION: The changes in BB EMG variables indicated exercise caused myoelectric manifestations of fatigue, however, acute moderate or severe hypoxia had no additional influence on the rate of fatigue development or neuromuscular parameters.

Effect of moderate and Severe Hypoxic exposure coupled with fatigue on psychomotor vigilance testing, muscle tissue oxygenation, and muscular performance.

PURPOSE: The purpose of this study is to examine the effects of fatigue on muscular performance, oxygenation saturation, and cognition following acute hypoxic exposure at Normoxia, Moderate Hypoxia (MH), and Severe Hypoxia (SH). METHODS: Twelve males performed 3 sets of leg extensions to failure under Normoxia (FiO2: 21%), MH (Fi02: 15.4%), and SH (Fi02: 12.9%). Heart rate, peripheral oxygenation saturation, total saturation index, psychomotor vigilance testing reaction time, psychomotor vigilance error rate, maximum strength, and repetitions to failure were measured throughout each visit. RESULTS: The primary findings indicated that MH and SH resulted in significant decreases in psychomotor vigilance test performance (MH: 388.25-427.17 ms, 0.41-0.33 error rate; SH: 398.17-445.42 ms reaction time, 0.25-1.00 error rate), absolute muscle tissue oxygen saturation (Abs-StO2) (MH:67.22% compared to SH:57.56%), but similar muscular strength, heart rate, and patterns of muscle tissue oxygen saturation responses (StO2%) during fatigue when compared to Normoxia. There was an acute decrease in the ability to remain vigilant and/or respond correctly to visual stimuli as indicated by the worsened reaction time (PVTRT) during MH (FiO2: 15.4%) and increased PVTRT and error rate (PVTE) during SH (FiO2: 12.9%) conditions. CONCLUSIONS: Acute hypoxic exposure in the current study was not a sufficient stimuli to elicit hypoxic-related changes in HR, muscular strength (1-RM), or repetitions to failure. The SpO2 responses were hypoxic-level dependent with increasing levels of hypoxia resulting in greater and more sustained reductions in SpO2. The combined SpO2 and StO2 responses at MH and SH suggested a balance between the muscles metabolic demand remaining lower than the muscle oxygen diffusion capacity. During the SH condition, Abs-StO2 suggested greater metabolic stress than Normoxia and MH conditions during the fatiguing leg extensions. The patterns of responses for StO2% during the three sets of leg press to failure indicated that exercise is a more potent influencer to muscle oxygenation status than hypoxic conditions (FiO2: 15.4 and 12.9%).