ABSTRACT
Development of cutaneous carcinomas has been associated with HPV infection. There have been various reports on p16, p53 and pRb expression in cutaneous carcinomas and on its linkage to HPV status. Association of protein expression and HPV infection with DNA content is not clear. The aim of this study was to determine a possible correlation between HPV type, protein expression and DNA content in both pre-invasive and invasive squamous cell carcinoma, as well as differences between studied groups in these parameters. Sections of formalin fixed paraffin-embedded tumor tissue from 54 cases of Morbus Bowen (preinvasive cutaneous carcinoma) and 41 cases of invasive squamous cell carcinoma of the skin were subjected to HPV genotyping using Lipa (Line imuno probe assay), immunohistochemical staining for p16(INK4A), p53, pRb and prepared for flow cytometry DNA content analysis. Obtained data were analyzed in SPSS using Chi square test. Only p16 expression showed statistically significant differences in studied groups. Statistically significant correlations were found only in MB between parameters HPV-p53, p53-pRb and p53-p16. Our results suggest different virus-induced pathobiology pathways for different cutaneous carcinoma groups.
Subject(s)
Carcinoma in Situ/genetics , Carcinoma, Squamous Cell/genetics , Papillomaviridae/genetics , Papillomavirus Infections/genetics , Skin Neoplasms/genetics , Tumor Suppressor Proteins/genetics , Aged , Aged, 80 and over , Carcinoma in Situ/metabolism , Carcinoma in Situ/virology , Carcinoma, Squamous Cell/metabolism , Carcinoma, Squamous Cell/virology , Croatia , Cyclin-Dependent Kinase Inhibitor p16/genetics , Cyclin-Dependent Kinase Inhibitor p16/metabolism , DNA, Neoplasm/genetics , DNA, Neoplasm/metabolism , DNA, Viral/genetics , DNA, Viral/metabolism , Female , Flow Cytometry , Host-Pathogen Interactions/genetics , Humans , Male , Middle Aged , Papillomaviridae/physiology , Papillomavirus Infections/metabolism , Papillomavirus Infections/virology , Retinoblastoma Protein/genetics , Retinoblastoma Protein/metabolism , Skin Neoplasms/metabolism , Skin Neoplasms/virology , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism , Tumor Suppressor Proteins/metabolismSubject(s)
DNA, Viral/analysis , Neoplasm Recurrence, Local/diagnosis , Papillomaviridae/genetics , Papillomavirus Infections/diagnosis , Uterine Cervical Dysplasia/diagnosis , Uterine Cervical Neoplasms/diagnosis , Adolescent , Adult , Aged , Combined Modality Therapy , Female , Follow-Up Studies , Humans , Male , Middle Aged , Neoplasm Recurrence, Local/therapy , Neoplasm Recurrence, Local/virology , Neoplasm Staging , Papillomaviridae/isolation & purification , Papillomavirus Infections/complications , Papillomavirus Infections/therapy , Papillomavirus Infections/virology , Prognosis , Uterine Cervical Neoplasms/therapy , Uterine Cervical Neoplasms/virology , Young Adult , Uterine Cervical Dysplasia/therapy , Uterine Cervical Dysplasia/virologyABSTRACT
This study examines anthropometrically assessed nutritional status of the Bayash, the Roma population from the eastern Croatian region of Baranya, and compares it to the non-Roma general population of eastern Croatia. The analysis of nutritional status and diets is a segment of multidisciplinary anthropological and epidemiological survey of the Roma minority population in Croatia began in 2005. The Bayash are an ethnic group that arrived to Croatia from Romania most likely in the 19th century and speaks a distinct archaic dialect of the Romanian language. The Roma population of Baranya approximates 1,000 according to the 2001 census. The Bayash sample comprised 227 adults aged 18-65yrs. The women fall below the Croatian 10th percentile for stature and men track about the 10th percentile. Both sexes approximate the 25th percentile for body weight. Despite their diminutive size, the Bayash appear to have adequate nutritional status until the age of 35yrs after which their average BMI exceeds the value of 25 kg/m(2) and falls in the overweight category. However, 8% of Bayash are underweight (BMI<18.5) in contrast to 1% of the majority population in the region. Underweight rates are especially high in women (11%) compared to men (4%). The prevalence of overweight (BMI 25.0 to 29.9) of 30% is considerably lower than in the majority population (42%) while the prevalence of obesity (BMI>or=30.0) of 23% is approximately equal. Overall unsatisfactory nutritional status of the Bayash merits attention. It appears to be the product of unhealthy dietary habits and their socio-economic deprivation that resulted from their poor education and extremely high unemployment.
