ABSTRACT
The aim of the study is in vitro investigation of the feasibility of surface-modified bacterial nanofibrous poly [(R)-3-hydroxybutyrate] (PHB) graft for bladder reconstruction. In this study, the surface of electrospun bacterial PHB was modified with PEG- or EDA via radio frequency glow discharge method. After plasma modification, contact angle of EDA-modified PHB scaffolds decreased from 110 ± 1.50 to 23 ± 0.5 degree. Interestingly, less calcium oxalate stone deposition was observed on modified PHB scaffolds compared to that of non-modified group. Results of this study show that surface-modified scaffolds not only inhibited calcium oxalate growth but also enhanced the uroepithelial cell viability and proliferation.
Subject(s)
Biocompatible Materials/pharmacology , Epithelial Cells/drug effects , Hydroxybutyrates/pharmacology , Regeneration/drug effects , Urinary Bladder/drug effects , Animals , Biocompatible Materials/isolation & purification , Biocompatible Materials/metabolism , Calcium Oxalate/chemistry , Cell Line , Cell Proliferation/drug effects , Cell Survival/drug effects , Cupriavidus necator/chemistry , Cupriavidus necator/metabolism , Epithelial Cells/pathology , Ethylenediamines/chemistry , Hydroxybutyrates/isolation & purification , Hydroxybutyrates/metabolism , Kidney Calculi/chemistry , Mice , Polyethylene Glycols/chemistry , Polymerization , Tissue Engineering , Urinary Bladder/pathologyABSTRACT
Olive oil mill wastewater (OMWW), a recalcitrant pollutant, has features including high phenolic content and dark color; thereby, several chemical or physical treatments or biological processes were not able to remediate it. In this study, the treatment efficiencies of three treatments, including adsorption, biological application, and photo-Fenton oxidation were sequentially evaluated for OMWW. Adsorption, biological treatment, and photo-Fenton caused decreasing phenolic contents of 48.69 %, 59.40 %, and 95 %, respectively. However, after three sequential treatments were performed, higher reduction percentages in phenolic (total 99 %) and organic contents (90 %) were observed. Although the studied fungus has not induced significant color reduction, photo-Fenton oxidation was considered to be an attractive solution, especially for color reduction. Besides, toxicity of OMWW treatment was significantly reduced.
Subject(s)
Hydrogen Peroxide/chemistry , Iron/chemistry , Plant Oils/chemistry , Plant Oils/metabolism , Trametes/metabolism , Waste Disposal, Fluid/methods , Water Pollutants, Chemical/chemistry , Water Pollutants, Chemical/metabolism , Adsorption , Aliivibrio fischeri/drug effects , Industrial Waste/analysis , Luminescence , Olive Oil , Oxidation-Reduction , Plant Oils/toxicity , Turkey , Water Pollutants, Chemical/toxicityABSTRACT
Polyhydroxyalkanoates (PHA) are natural, thermoplastic polyesters and due to their biocompatible and biodegradable properties they are good alternatives for the production of scaffolds for engineered tissues or nanoparticles for drug delivery. As a member of polyhydroxyalkanoate family, polyhydroxybutyrates (PHB) have been widely used as a biomaterial for in vitro and in vivo studies since their mechanical properties are very similar to conventional plastics. By using multi-emulsion technique, iron oxide particles were coated with polyhydroxybutyrate (PHB) polymer synthesized from Alcaligenes eutrophus bacteria and the magnetic carrier system was prepared accordingly. The bare nanoparticles and magnetic nanoparticles were morphologically, structurally and magnetically characterized by using Scanning electron microscope (SEM) and Atomic force microscope (AFM); Fourier Transform Infrared Spectrometry (FTIR), and Electron Spin Resonance (ESR) and Vibrating Sample Magnetometer (VSM) techniques, respectively. Particle size of PHB nanoparticles was determined by Zeta Sizer. It was found that the smallest particles were in the range of 239.43 +/- 5.25 nm in diameter. Concanavalin-A (Con-A) was used for targeting the cancer cells while etoposide was used as drug. Con-A and etoposide were loaded onto the particles. Release studies of etoposide were evaluated and the system was optimized for the further in vivo applications. Finally different formulation magnetic PHB nanoparticles cytotoxicity were evaluated in cell culture studies and used HeLa cell line (cervical cancer cells) as a cancer cells and L929 cells (mouse fibroblast cells) as a non-cancer cell line.
