ABSTRACT
AIM: Analysis of epidemic manifestations of natural-foci infections (NFI), clarification of spectrum of their causative agents, determination of epizootic activity of natural foci in the Crimea Federal District (KFD). MATERIALS AND METHODS: Epizootologic examination of 10 administrative districts of KDF was carried out. 291 pools (2705 specimens) of ixodes ticks and 283 samples of organs of small mammals were studied by PCRmethod for the presence of DNA/RNA of causative agents of a number of NFI. RESULTS: Morbidity by NFI in KFD was registered by 6 nosologies: Lyme borreliosis, Marseilles fever, leptospirosis; tularemia, intestine yersiniosis and tick-borne viral encephalitis, wherein, transmissive infections made up 91.6%. Circulation of causative agents of Crimea hemorrhagic fever, Q fever, group of tick-borne spotted fever, Lyme borreliosis, human granulocytic anaplasmosis, human monocytic ehrlichiosis, hemorrhagic fever with renal syndrome, West Nile fever, tularemia and leptospirosis was established. CONCLUSION: Due to activity of natural foci of NFI further monitoring of epidemiologic and epizootologic manifestations of these infections in the Crimea, including using genetic methods of analysis, is necessary for ensuring sanitary-epidemiologic welfare of KFD population.
Subject(s)
Focal Infection/epidemiology , Lyme Disease/epidemiology , Tick-Borne Diseases/epidemiology , Viruses/isolation & purification , West Nile Fever/epidemiology , Animals , DNA, Viral/genetics , DNA, Viral/isolation & purification , Disease Reservoirs , Focal Infection/genetics , Focal Infection/virology , Humans , Ixodes/pathogenicity , Ixodes/virology , Lyme Disease/genetics , Lyme Disease/virology , Mammals/virology , Russia , Tick-Borne Diseases/genetics , Tick-Borne Diseases/virology , Viruses/genetics , Viruses/pathogenicity , West Nile Fever/geneticsABSTRACT
The character of drug interaction in 18 various combinations of sisomicin, amikacin, gentamicin, cefotaxime, doxycycline, trimethoprim and nitroxaline was studied by the chess board method with respect to 25 strains of Brucella melitensis. The synergistic effect with respect to the majority of the cultures was observed with the use of the combinations of gentamicin, amikacin or sisomicin with doxycycline. The combinations of the aminoglycosides with cefotaxime and sisomicin or gentamicin with rifampicin had a summation effect on the majority of the cultures. With the use of the combinations of cefotaxime with nitroxaline or trimethoprim and rifampicin with nitroxaline the effect on the majority of the cultures was indifferent. The effect was mainly antagonistic with the use of the combinations of amikacin with rifampicin, gentamicin with trimethoprim and cefotaxime with doxycycline. The effect of the combinations of gentamicin with nitroxaline and cefotaxime with rifampicin was different with respect to different strains of B. melitensis. It was indifferent, antagonistic or summation depending on the strain.
Subject(s)
Anti-Bacterial Agents/pharmacology , Brucella melitensis/drug effects , Animals , Brucella melitensis/isolation & purification , Drug Interactions , Drug Therapy, Combination/pharmacology , Humans , Microbial Sensitivity TestsABSTRACT
In vitro activity of 13 antibiotics and drug preparations as well as some of their combinations was studied with respect to Brucella melitensis. It was shown that the strains of B. melitensis were sensitive to sisomicin, amikacin, gentamicin, cefotaxime, rifaprim +, nitroxoline and trimethoprim. Variations in the doxycycline sensitivity depending on the strain were observed. The strains of B. melitensis were resistant to cephalexin, phosphomycin, chinoxydine, dioxydine and oxolinic acid. Combinations of sisomicin and trimethoprim at a ratio of 1:10 and sisomicin and nitroxoline at a ratio of 1:2 had a summation effect.
