ABSTRACT
PA were found to promote creation of the NF-kappaB complex with the correspondent DNA sequences (NRE), p50 NF-kappaB-subunit affinity with NRE is essentially magnified in PA presence. This is one of important demonstrations of PA involvement in transcription control. NF-kappaB activity in experimental mammary tumors cells was shown to depend on PA metabolism. Retardation of experimental mammary tumors growth caused by the inhibitors of PA biosynthesis was accompanied with decreased expression of protein products of the NF-kappaB-dependent genes such as c-myc, bcl-xl. It was hypothesized that under PA depletion not classic NF-kappaB (p50/p65 heterodimer) but p50/p50 homodimer is mainly created, and this homodimer, because of PA deficiency, is not able to promote transcription of the gene have been tested.
Subject(s)
Biogenic Polyamines/physiology , Cell Proliferation , NF-kappa B/biosynthesis , Transcription, Genetic , Animals , Antineoplastic Agents/pharmacology , Biogenic Polyamines/biosynthesis , Biogenic Polyamines/metabolism , Blotting, Western , Cell Line, Tumor , Cell Nucleus/drug effects , Cell Nucleus/metabolism , Cell Proliferation/drug effects , Computer Simulation , Eflornithine/pharmacology , Electrophoresis, Polyacrylamide Gel , Humans , Mice , Models, Biological , NF-kappa B/genetics , Polymerase Chain ReactionABSTRACT
Green tea biocomposite had effectivey hampered the growth of rat Walker W-256 carcinoma and in less extent rat Guerin's carcinoma. Black tea biocomposite had not practically influenced on Guerin's carcinoma growth. The biocomposite from green tea and extract from red vine rind and lemon suppressed at the level of tendency the growth of rat Walker W-256 carcinoma. The biocomposite from green tea and extract from red vine rind had hampered only Guerin's carcinoma growth and at the tendency had increased the growth of W-256 carcinosarcoma growth. This biocomposite increased also considerably the therapeutic efficiency of cisplatin on Guerin's carcinoma. Studed vegetable biocomposites posesses antiinflammatory and antioxidant properties.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Carcinoma 256, Walker/drug therapy , Neoplasms, Hormone-Dependent/drug therapy , Tea/chemistry , Animals , Antineoplastic Agents, Phytogenic/administration & dosage , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/therapeutic use , Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Cell Line, Tumor , Cisplatin/administration & dosage , Cisplatin/therapeutic use , Drug Screening Assays, Antitumor , Female , Flavonoids/administration & dosage , Flavonoids/isolation & purification , Flavonoids/therapeutic use , Neoplasm Transplantation , Neoplasms, Experimental/drug therapy , Phenols/administration & dosage , Phenols/isolation & purification , Phenols/therapeutic use , Polyphenols , Rats , Rats, WistarABSTRACT
Moderate consumption of a curd-like product made of thermally-treated soy (SPT) led to the retardation of hormone-dependent (Walker W 256 carcinosarcoma in females) and some less hormone-independent (Guerin's carcinoma in males) tumor growth in rats. Excessive (ad libitum) consumption of the same product led to accelaration of W 256 tumor growth. A similar product made of raw soy (SPR) accelerated the growth of W-256 carcinosarcoma and made not any effect on the growth of Guerin's carcinoma. Moderate SPT consumption corrected erythropoesis, decreased lipids peroxidation, retarded peritumoral inflammation, decreased or not changed the content of direct bilirubin in blood serum. SPRconsumption did not lead to those positive effects but sometimes deteriorated those indices. Our experiments have also shown the express-test validity based on dynamical variant of cancerolysis reaction to be practical for evaluation of food quality for cancer patients.
