ABSTRACT
OBJECTIVE: Vanillosmopsis arborea Baker (Asteraceae) has high economic value from Chapada to Araripe and its bark essential oil is a potential source of alpha-bisabolol. The present study aimed to elucidate the antinociceptive and antipruritic properties of the essential oil of V. arborea Baker (EOVA) in mice. MATERIALS AND METHODS: The antinociceptive activity was assessed using the capsaicin, glutamate, hot plate and cold allodynia tests. The antipuritic effects were also verified based on histamine-induced scratching behavior. RESULTS: EOVA reduced the paw licking induced by capsaicin, but not that induced by glutamate. The essential oil increased the latency time in the hot plate, attenuated the cold allodynia induced by acetone and inhibited histamine-induced scratching behavior. CONCLUSION: The experimental data demonstrated that EOVA showed central and peripheral antinociceptive activity and antipruritic effect.
ABSTRACT
This study aimed to assess the possible topical antinociceptive activity of Vanillosmopsis arborea Baker essential oil (EOVA) and to clarify the underlying mechanism, using the acute model of chemical (eye wiping) nociception in mice. EOVA (25 to 200 mg/kg; p.o. and topical) evidenced significant antinociception against chemogenic pain in the test model of formalin-induced neuroinflammatory pain. Local application of 5 M NaCl solution on the corneal surface of the eye produced a significant nociceptive behavior, characterized by eye wiping. The number of eye wipes was counted during the first 30 s. EOVA (25, 50, 100, and 200 mg/kg; p.o. and topical) significantly decreased the number of eye wipes. Naloxone, yohimbine, L-NAME, theophylline, glibenclamide, and ruthenium red had no effect on the antinociceptive effect of EOVA. However, ondansetron, p-chlorophenylalanine methyl ester (PCPA), capsazepine, prazosin, and atropine prevented the antinociception induced by EOVA. These results indicate the topical antinociceptive effect of EOVA and showed that 5-HT, α 1, TRPV1, and central muscarinic receptors might be involved in the antinociceptive effect of EOVA in the acute corneal model of pain in mice.
ABSTRACT
BACKGROUND AND OBJECTIVES: Vanillosmopsis arborea Baker (Asteraceae) has high economic value and anti-inflammatory properties due the presence of alpha bisabololin its bark essential oil. Keeping in view the highcontent of ?-bisabolol in Vanillosmopsis arborea (EOVA) bark essential oil, the aim of our study was to determinewhether EOVA mitigates visceral nociception induced bydifferent noxious gents.METHOD: Mice (n = 8) were pretreated orally with EOVA (100, 200 e 400 mg/kg) or vehicle, and pain-related behavioral responses to intraperitoneal cyclophosphamide (CPM 400 mg/kg), intracolonic mustard oil (MO0.75%) or capsaicin (CAP 0.3%) were analyzed.RESULTS: Animals that received CFM, OM or CAP presented spontaneous nociceptive behaviors that were signifi cantly suppressed by EOVA. CONCLUSION: These fi ndings point to visceral antinociceptive properties of EOVA suggesting the potential use of Vanillosmopsis arborea to treat pain associated to gastrointestinal disorders
JUSTIFICATIVA E OBJETIVOS: Vanillosmopsis arborea Baker (Asteraceae) de reconhecido valor econômico que possui propriedades anti-inflamatórias provenientes do sesquiterpeno alfa-bisabolol, presente em teores elevados no óleo essencial de sua madeira. Considerando-se o alto teor de ?-bisabolol no óleo essencialdo caule de Vanillosmopsis arborea (OEVA). O objetivo deste estudo foi determinar o efeito do OEVA na nocicepção visceral induzida por diversos agentes irritantes.MÉTODO: Camundongos (n = 8) foram pré-tratados por via oral com EOVA (100, 200 e 400 mg/kg) ou veículo e as respostas comportamentais devido a administração de ciclofosfamida (CFM 400 mg/kg; por via intraperitoneal), óleo de mostarda (OM 0,75%; intracolônico) ou capsaicina (CAP 0,3%; intracolônica) foram registradas.RESULTADOS: Os animais que receberam CFM, OM ou CAP manifestaram comportamentos nociceptivos espontâneos que foram significativamente suprimidos nosgrupos tratados com OEVA.CONCLUSÃO: Estes achados apontam a propriedade antinociceptiva visceral do óleo essencial do caule de Vanillosmopsis arborea indicando o uso potencial no alívio da dor associada às desordens gastrintestinais.
