ABSTRACT
La conjuntivitis es la enfermedad ocular más extendida en el mundo. Constituye la respuesta de la conjuntiva a una gran variedad de agresiones: bacteriana, vírica, micótica, parasitaria, alérgica, tóxica y mecánica. El propósito de este estudio es mostrar algunos casos de conjuntivitis infecciosas en neonatos y poner en evidencia su etiología bacteriana. Este estudio comprende seis casos en los que se realizó examen citobacteriológico, cultivo y antibiograma en la Unidad de Microbiología del Hospital General Universitario de Valencia. Sus edades oscilaban entre 27 horas y 6 días (AU)
Subject(s)
Female , Male , Humans , Infant, Newborn , Conjunctivitis/complications , Conjunctivitis/diagnosis , Conjunctivitis/drug therapy , Vaginosis, Bacterial/complications , Vaginosis, Bacterial/epidemiology , Vaginosis, Bacterial/prevention & control , Microbial Sensitivity Tests/methods , Microbial Sensitivity Tests , Conjunctivitis, Bacterial/diagnosis , Conjunctivitis, Bacterial/etiology , Conjunctivitis, Bacterial/epidemiology , Jaundice, Neonatal/complications , Jaundice, Neonatal/diagnosis , Jaundice, Neonatal/etiology , Ciprofloxacin/administration & dosage , Ciprofloxacin/therapeutic useABSTRACT
Staphylococci are frequently isolated from human, animal and environmental samples. The genus Staphylococcus comprises 27 species, of which 23 are coagulase-negative. The pathogenic role of the coagulase-negative species remains to be fully established. Staphylococcus lugdunensis is a recently described coagulase-negative species. Its Latin name reflects the city (Lyon, France) where the bacteria was first described. The present study reports 7 cases of human infection caused by S. lugdunensis. Case 1: a 64 y/o diabetic woman in whom S. lugdunensis was isolated in the conjunctival exudate. Case 2: a 51 y/o male diagnosed of a bladder tumor, with S. lugdunensis detected in the purulent exudate of a transurethral resection. Case 3: an 81 y/o woman with cerebrovascular stroke, in whom S. lugdunensis was isolated from urine. Case 4: a 65 y/o male with bacterial endocarditis and S. lugdunensis isolation in blood culture. Case 5: a 79 y/o male with liver cirrhosis and S. lugdunensis in blood culture. Case 6: a 75 y/o female with cerebrovascular stroke, in whom S. lugdunensis was isolated from urine. Case 7: a 77 y/o male diagnosed of femoral osteosarcoma and isolation of S. lugdunensis in the purulent exudate of the wound. The present communication broadens the range of clinical syndromes in which coagulase-negative S. lugdunensis appears as pathogen.
Subject(s)
Staphylococcal Infections/microbiology , Staphylococcus/isolation & purification , Aged , Coagulase/deficiency , Female , Humans , Male , Middle Aged , Staphylococcal Infections/diagnosis , Staphylococcus/enzymologyABSTRACT
Los estafilococos se aislan frecuentemente en muestras humanas, animales y del medio ambiente. Existen 27 especies dentro del género Staphylococcus, de ellas, 23 son coagulasa negativas. El papel patógeno de las especies coagulasa negativas no está todavía bien asentado. Staphylococcus lugdunensts es una especie coagulasa negativa descrita recientemente. Su nombre viene del latín lugdunum, que significa Lyon, por haber sido en esta ciudad donde se describió por primera vez. El objeto de la presente comunicacion es describir 7 casos humanos de infección por esta bacteria. Caso 1: paciente diabetica de 64 años en la que se aisló S. lugdunensis en el exudado conjuntival. Caso 2: paciente varón de 51 años diagnosticado de neoformación vesical, aislando S. lugdunensis en el exudado purulento de una resección transuretral. Caso 3: paciente de 81 años diagnosticada de accidente cerebrovascular isquémico, con S. lugdunensis aislado en orina. Caso 4: paciente de 65 años diagnosticado de endocarditis bacteriana, aislándose S. lugdunensis en hemocultivo. Caso 5: paciente de 79 años con cirrosis hepática y aislamiento de S. lugdunensis en hemocultivo. Caso 6: paciente de 75 años diagnosticada de accidente cerebrovascular, aislando S. lugdunensis en orina. Caso 7: varón de 77 años con osteosarcoma de fémur y aislamiento de S. lugdunensis en el exudado purulento de la herida. La presente comunicación expande los síndromes clínicos en los que S. lugdunensis, estafilococo coagulasa negativo, aparece como bacteria patógena (AU)
Subject(s)
Aged , Female , Male , Middle Aged , Humans , Coagulase/deficiency , Staphylococcal Infections/diagnosis , Staphylococcus/enzymology , Staphylococcal Infections/microbiology , Staphylococcus/isolation & purificationABSTRACT
Urinary infections caused by Staphylococcus are attributed usually to Staphylococcus epidermidis or Staphylococcus saprophyticus. 8 cases of urinary infection due to S. haemolyticus are discussed: 5 adults all of them over 66 years, diagnosed respectively of diabetes, cancer and stroke. Three children diagnosed of febrile syndrome, encephalopathy and enterocolitis associated with urinary infection. S. haemolyticus was identified through the determination of 19 biochemical parameters. The antibiograms performed showed that all S. haemolyticus isolated were sensible to vancomycin, nitrofurantoin and trimethoprim/sulfamethoxazole. It is concluded that S. haemolyticus is an opportunistic pathogen that is present on immunodepressed hosts, that is why is advisable to perform a more in-depth characterization of the S. epidermidis isolated in urine, especially in persistent pathologic processes.
Subject(s)
Opportunistic Infections/microbiology , Staphylococcal Infections/microbiology , Staphylococcus/isolation & purification , Urinary Tract Infections/microbiology , Aged , Aged, 80 and over , Anti-Bacterial Agents/pharmacology , Bacteriuria/complications , Bacteriuria/microbiology , Female , Humans , Infant , Male , Microbial Sensitivity Tests , Opportunistic Infections/complications , Staphylococcal Infections/complications , Staphylococcus/drug effects , Urinary Tract Infections/complicationsABSTRACT
A clinical case of a 58 year old male suffering from hepatitis non A, non B, and terminal chronic renal insufficiency under periodic dialysis, is presented. Following a 600 cc of packed red blood cells transfusion, there was an increase of antibodies IgG against cytomegalovirus (from 1/160 to 1/1560), detected by ELISA. At the same time, there were an increase of transaminases up to 404 UI/ml of GPT. Total bilirubin persisted normal. The possible etiology of the hepatitis non A, non B caused by cytomegalovirus is discussed and the results compared to those observed by other authors in immunosuppressed renal transplant patients.
Subject(s)
Cytomegalovirus Infections/complications , Hepatitis C/complications , Humans , Male , Middle AgedABSTRACT
We study 18 children with acute respiratory infection diagnosed early by direct immunofluorescence (DIF) using monoclonal antibodies to synticial respiratory virus (SRV). Antibodies to adenovirus, influenza virus A and B, parainfluenza virus 1, 2 and 3, mycoplasma, Q fever, herpes and cytomegalovirus (CMV) are also studied. We consider that in most cases the diagnosis of infections caused by SRV cannot be established by complement fixation, and should be accompanied by some antigen detection technique. Direct immunofluorescence facilitates the early detection of SRV in most cases before the appearance of antibodies to this virus. Only one of the 18 patients with positive DIF presented antibodies to SRV.
Subject(s)
Respiratory Syncytial Viruses/immunology , Respirovirus Infections/diagnosis , Antibodies, Monoclonal/immunology , Fluorescent Antibody Technique , Humans , Infant , Infant, Newborn , Respirovirus Infections/immunologyABSTRACT
Nine cases of respiratory infections caused by syncytial respiratory virus which were diagnosed by direct immunofluorescence with monoclonal antibodies are presented here. Six of them have been clinically diagnosed as bronchiolitis and the remaining three are diagnosed as obstructive bronchitis, upper respiratory cold and respiratory distress respectively. Seven are male and two are female. Their ages vary from 13 days to eleven months. Its usefulness is indicated given its speed and the current availability of specific antiviral therapeutics. The test would be especially indicated in atypical cases like nursing babies with apnea or those with congenital heart disease or other underlying lung diseases and also to prevent intrahospital epidemias. The conclusion is reached that direct immunofluorescence seems to be an attractive alternative for laboratories that do not have costly cell culture equipment.
Subject(s)
Respiratory Syncytial Viruses , Respirovirus Infections/diagnosis , Female , Humans , Infant , Infant, Newborn , MaleABSTRACT
The authors reports the results of ELISA IgG and IgA antigliadin antibodies measurements in a study of 271 serum samples proceeding from celiac patients (with and without gluten containing diet) and control subjects. IgA antigliadin antibody measurement had the most specificity and positive predictive value, IgG antigliadin antibody measurement had the most sensitivity and negative predictive value. Our results point out that antigliadin antibodies are helpful in the diagnosis and management of celiac disease. Un the same manner, antigliadin antibodies are helpful to evaluate the adherence of patients to gluten-free diet.
Subject(s)
Celiac Disease/immunology , Gliadin/immunology , Glutens/adverse effects , Immunoglobulin A/immunology , Immunoglobulin G/immunology , Plant Proteins/immunology , Celiac Disease/diagnosis , Celiac Disease/diet therapy , Child , Enzyme-Linked Immunosorbent Assay , HumansSubject(s)
Acquired Immunodeficiency Syndrome/complications , Antibodies, Viral/analysis , Cytomegalovirus Infections/complications , Immunoglobulin M/analysis , Opportunistic Infections/complications , Acquired Immunodeficiency Syndrome/immunology , Adult , Cytomegalovirus/immunology , Humans , Male , PrognosisSubject(s)
Hypertension/epidemiology , Personnel, Hospital , Adult , Age Factors , Aged , Female , Humans , Male , Middle Aged , RiskSubject(s)
Eosinophilia/etiology , Filariasis/complications , Loiasis/complications , Adult , Female , HumansABSTRACT
Present survey has analysed the results obtained from 13 children whose FTA-abs (IgG) test proved to be positive. For assessing their anti-Treponemal and reaginic antibodies, the RPR, passive hemagglutination (MHA-TP) and FTA-abs trials were carried out, the existence of specific IgG and IgM being noticed from the latter. Out of the 13 samples subject to our analyses, 7 showed some specific IgM antibodies, with the existence of a congenital syphilis being clinically assessed from three of them, whereas the remaining 6 samples only showed some IgG antibodies. The need for an early diagnosis during the first and third quarters of pregnancy is indicated, through a reaginic (RPR o VDRL) trial or a treponemic (MHA-TP) one, as well as the assessing of anti-Treponema pallidum antibodies in the umbilical cord blood, where a positive luetic serology appears to be the case during the period of pregnancy.
