Abstract perceptual choice signals during action-linked decisions in the human brain.

Humans can make abstract choices independent of motor actions. However, in laboratory tasks, choices are typically reported with an associated action. Consequentially, knowledge about the neural representation of abstract choices is sparse, and choices are often thought to evolve as motor intentions. Here, we show that in the human brain, perceptual choices are represented in an abstract, motor-independent manner, even when they are directly linked to an action. We measured MEG signals while participants made choices with known or unknown motor response mapping. Using multivariate decoding, we quantified stimulus, perceptual choice, and motor response information with distinct cortical distributions. Choice representations were invariant to whether the response mapping was known during stimulus presentation, and they occupied a distinct representational space from motor signals. As expected from an internal decision variable, they were informed by the stimuli, and their strength predicted decision confidence and accuracy. Our results demonstrate abstract neural choice signals that generalize to action-linked decisions, suggesting a general role of an abstract choice stage in human decision-making.

Testing the generalization of neural representations.

Multivariate analysis methods are widely used in neuroscience to investigate the presence and structure of neural representations. Representational similarities across time or contexts are often investigated using pattern generalization, e.g. by training and testing multivariate decoders in different contexts, or by comparable pattern-based encoding methods. It is however unclear what conclusions can be validly drawn on the underlying neural representations when significant pattern generalization is found in mass signals such as LFP, EEG, MEG, or fMRI. Using simulations, we show how signal mixing and dependencies between measurements can drive significant pattern generalization even though the true underlying representations are orthogonal. We suggest that, using an accurate estimate of the expected pattern generalization given identical representations, it is nonetheless possible to test meaningful hypotheses about the generalization of neural representations. We offer such an estimate of the expected magnitude of pattern generalization and demonstrate how this measure can be used to assess the similarity and differences of neural representations across time and contexts.

Neural representations of the content and production of human vocalization.

Speech, as the spoken form of language, is fundamental for human communication. The phenomenon of covert inner speech implies functional independence of speech content and motor production. However, it remains unclear how a flexible mapping between speech content and production is achieved on the neural level. To address this, we recorded magnetoencephalography in humans performing a rule-based vocalization task. On each trial, vocalization content (one of two vowels) and production form (overt or covert) were instructed independently. Using multivariate pattern analysis, we found robust neural information about vocalization content and production, mostly originating from speech areas of the left hemisphere. Production signals dynamically transformed upon presentation of the content cue, whereas content signals remained largely stable throughout the trial. In sum, our results show dissociable neural representations of vocalization content and production in the human brain and provide insights into the neural dynamics underlying human vocalization.

Monkey EEG links neuronal color and motion information across species and scales.

It remains challenging to relate EEG and MEG to underlying circuit processes and comparable experiments on both spatial scales are rare. To close this gap between invasive and non-invasive electrophysiology we developed and recorded human-comparable EEG in macaque monkeys during visual stimulation with colored dynamic random dot patterns. Furthermore, we performed simultaneous microelectrode recordings from 6 areas of macaque cortex and human MEG. Motion direction and color information were accessible in all signals. Tuning of the non-invasive signals was similar to V4 and IT, but not to dorsal and frontal areas. Thus, MEG and EEG were dominated by early visual and ventral stream sources. Source level analysis revealed corresponding information and latency gradients across cortex. We show how information-based methods and monkey EEG can identify analogous properties of visual processing in signals spanning spatial scales from single units to MEG - a valuable framework for relating human and animal studies.