ABSTRACT
All-trans retinoic acid (ATRA) is controversially discussed in emphysema therapy. We re-evaluated ATRA in the elastase model and hypothesised that beneficial effects should be reflected by increased alveolar surface area, elastin expression and downregulation of inflammatory mediators and matrix metalloproteinases (MMPs). Emphysema was induced by porcine pancreatic elastase versus saline in Sprague-Dawley rats. On days 26-37, rats received daily intraperitoneal injections with ATRA (500 µg · kg(-1) body weight) versus olive oil. Lungs were removed at day 38. Rat alveolar epithelial L2 cells were incubated with/without elastase followed by ATRA- or vehicle-treatment, respectively. ATRA only partially ameliorated structural defects. Alveolar walls exhibited irregular architecture: increased arithmetic mean thickness, reduction in surface coverage by alveolar epithelial cells type II. ATRA only partially restored reduced soluble elastin. It tended to increase the ratio of ED1(+):ED2(+) macrophages. Bronchoalveolar lavage (BAL) cells exhibited a proinflammatory state and high expression of interleukin-1ß, cytokine-induced neutrophil chemoattractant-1, tumour necrosis factor-α, nuclear factor-κB, MMP-2, MMP-9, MMP-12, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in emphysema, with ATRA exerting only few effects. MMP-7 was highly induced by ATRA in healthy but not in emphysematous lungs. ATRA reduced both MMP-2 and TIMP-1 activity in BAL fluid of emphysematous lungs. ATRA-therapy may bear the risk of unwanted side-effects on alveolar septal architecture in emphysematous lungs.
Subject(s)
Emphysema/drug therapy , Macrophages/drug effects , Pulmonary Alveoli/drug effects , Tretinoin/therapeutic use , Animals , Bronchoalveolar Lavage Fluid/chemistry , Cell Line , Ectodysplasins/analysis , Elastin/analysis , Emphysema/chemically induced , Emphysema/enzymology , Emphysema/pathology , Interleukin-1beta/biosynthesis , Lung/chemistry , Lung/drug effects , Lung/enzymology , Lung/pathology , Macrophages/enzymology , Macrophages/pathology , Male , Matrix Metalloproteinase 12/biosynthesis , Matrix Metalloproteinase 2/biosynthesis , Matrix Metalloproteinase 7/biosynthesis , Matrix Metalloproteinase 9/biosynthesis , Pancreatic Elastase/toxicity , Pulmonary Alveoli/enzymology , Pulmonary Alveoli/pathology , Rats , Rats, Sprague-Dawley , Tissue Inhibitor of Metalloproteinase-1/biosynthesis , Tissue Inhibitor of Metalloproteinase-2/biosynthesis , Tumor Necrosis Factor-alpha/biosynthesisABSTRACT
Victims of Alzheimer's disease show a gradual and steady deterioration in memory, orientation, emotional stability, language capacity, abstract thinking, motor skills, and, ultimately, self care. Cognitive and motor deficits are accompanied by a gradual inability to perform adequate oral hygiene. Alzheimer's disease also interferes with the patient's ability to communicate dental symptoms of pain or dysfunction, and progressive deterioration of cognition interferes with the patient's ability to tolerate most therapeutic interventions. When treating patient's with Alzheimer's disease, oral health care providers must develop timely, preventive, and therapeutic strategies compatible with the patient's physical and cognitive ability to undergo and respond to dental care. They should strive to achieve those goals with the same ethical, moral, and professional standards of care as may be appropriate in the management of any other patient.
Subject(s)
Alzheimer Disease , Dental Care for Disabled , Aged , Aged, 80 and over , Alzheimer Disease/diagnosis , Alzheimer Disease/drug therapy , Alzheimer Disease/physiopathology , Alzheimer Disease/psychology , Dental Care for Disabled/classification , Dental Care for Disabled/methods , Dental Plaque/therapy , Dental Restoration, Permanent , Disease Progression , Ethics, Dental , Female , Humans , Male , Mouth Diseases/prevention & control , Mouth Diseases/therapy , Oral Hygiene , Patient Care Planning , Patient Care Team , Periodontal Diseases/therapy , Self Care , Tooth Diseases/prevention & control , Tooth Diseases/therapyABSTRACT
Anxiety symptoms are commonly reported both in patients and in the general population. There is also increasing concern being expressed over the widespread use of prescribed anxiolytics. This has encouraged the development of psychological interventions for both specific and generalised anxiety. In this paper, 29 patients, most of whom suffered from generalised or free-floating anxiety, were treated in small groups as part of a staff training programme. Their treatment was standardised, and consisted of progressive muscular relaxation, cognitive therapy and an educational input as to the nature of anxiety. Two measures, the Spielberger Trait Scale and an individualised problem rating scale were completed before and after the group and at three months follow-up. All but one of the results was statistically significant. The implications of these results are discussed.
