In vitro assessment of the pesticide bioaccessibility in Dendrobium officinale Kimura et Migo.

Bioaccessibility (BA) is a crucial factor for evaluating the absorption of pollutants in the human digestion system, which is of vital importance for risk assessment of pollutants via food intake. Multi-pesticides were detected in Dendrobium officinale Kimura et Migo (D. officinale), a popular dual-use plant with both botanical medicine and food applications. Nevertheless, the BA of pesticides in D. officinale remains unknown, restricting its market size. Herein, the BA of 19 pesticides with varying properties was evaluated by using an in vitro digestion model, showing BA values between 27.4 and 96.8%. The BA was controlled by the hydrophobicity and water solubility of pesticides, since the significant correlation between these two factors and BA values was observed. Moreover, co-ingested food ingredients could influence the BA, wherein the effect was significant for pesticides of logKow values no less than 3. Lipids enhanced the BA by 9-66%, whereas proteins or carbohydrates decreased BA values by 6-28%. In particular, considering the BA, the risk quotient values were reduced by 3-73%. Clearly, this work suggested that traditional risk assessment without considering the BA would seriously overestimate the actual risk of pesticides in food.

Simultaneous Determination of 108 Pesticide Residues in Three Traditional Chinese Medicines Using a Modified QuEChERS Mixed Sample Preparation Method and HPLC-MS/MS.

A rapid, efficient, simple, and high-throughput method for the simultaneous determination of 108 pesticide residues in three traditional Chinese medicines (TCMs) was established, comprising an improved QuEChERS method in combination with HPLC-MS/MS based on mixed samples. A quantity of 10 mL of acetonitrile was used as extraction solvent, and 10 mg of amino-modified multi-walled carbon nanotubes (MWCNTs-NH2) and 150 mg of anhydrous magnesium sulfate (MgSO4) were selected as sorbents for dispersive solid phase extraction. The performance of the method was verified according to the analytical quality control standards of SANTE/11813/2017 guidelines. With good linearity (R2 > 0.9984) in the range of 2−200 µg/L for all pesticides in the selected matrices, and good accuracy, precision, and high sensitivity, the recoveries were in the range of 70−120% for more than 95% of the pesticides, with a relative standard deviation (RSD) of less than 16.82% for all. The limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.01−3.87 µg/kg and 0.07−12.90 µg/kg, respectively, for Fritillaria thunbergii Miq (F. thunbergii), Chrysanthemum Morifolium Ramat (C. morifolium), and Dendrobium officinale Kimura et Migo (D. officinale). The method was successfully applied to 60 batches of actual samples from different regions.

A Novel Enantioseparation Method and Enantioselective Dissipation of Novaluron in Tomatoes Using Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry via a Box-Behnken Design.

Enantioseparation parameters of novaluron were optimized on a Chiralpak IG-3 column by ultrahigh-performance liquid chromatography tandem mass spectrometry via response surface methodology. The absolute configuration and elution order were identified as R-(+)-novaluron and S-(-)-novaluron by polarimetry and X-ray diffraction. A modified QuEChERS method was developed for enantioselective determination of novaluron in eight food and environmental samples. Under optimal conditions, the mean recoveries of the novaluron enantiomers in the eight matrices were 74.4-108.1% with intraday relative standard deviations (RSDs) of 0.3-9.7% and interday RSDs of 0.1-4.1%. Enantioselective dissipation was observed in tomatoes. The dissipation of S-(-)-novaluron was faster than that of R-(+)-novaluron with a half-life of 7.1 and 7.9 days, and the enantiomer fraction value changed from 0.49 to 0.53 in 21 days. An effective method for monitoring novaluron enantiomer residues in food and the environment was established for the first time and had been successfully applied to real samples. This study is of great significance for strengthening the risk assessment and supervision level of chiral pesticides.