The tide of tiger poaching in India is rising! An investigation of the intertwined facts with a focus on conservation.

Poaching and illegal trafficking are major threats to biodiversity, especially when endangered felids are concerned. Tigers are iconic animals, and there is huge demand for their body parts both in the national and international illegal markets. India forms the largest tiger conservation unit in the world and poaching is at its peak even though there are stringent laws and strict enforcement. In the present study, we analytically estimated the tiger seizure cases in India from 2001-2021 using newspaper archives as the main source of data. The data was geo-referenced to understand the details of seizure, demand, and locality. We statistically correlated the seizure rate with the density of tigers, tiger reserves, and various other socio-economic factors. Our result shows that skin, claws, bones, and teeth have more demand, with nails and teeth being the most preferred in local markets. The bones, flesh, and other parts were mostly seized in the border states of the north and eastern states. The intensity of seizures is very high in the states of Maharashtra, Karnataka, Tamil Nadu, and Assam. From our analysis, we predict four trade routes for the export of the seized parts: the Nepal-Bhutan border, Assam border, the Brahmaputra, and the Mumbai port. This corresponds to the five tiger conservation blocks in India, and we observed the seizure rate is high near the Western Ghats region, which has not yet been noticed. Apart from the seizure, we are unconcerned with the seizure's origin or the local trading routes. The study demonstrates the importance of identifying the source population using DNA methods and carefully enforcing the rules in area of poaching. We assert that current approaches are incapable of resolving the issue and that a more precise and effective forensic procedure capable of resolving the issue at the minute local level is critical for precisely tracing trade channels. Supplementary Information: The online version contains supplementary material available at 10.1007/s10708-022-10633-4.

Two new species and two new records of Onychiurinae (Collembola, Onychiuridae) from the Nilgiris, India.

Four species of Onychiurinae are reported from the Nilgiris, Western Ghats, southern India, including two new species, Thalassaphorura udagaiensis sp. nov. and Protaphorura sholai sp. nov., and two new records for India: Protaphorura fimata (Gisin, 1952) and Orthonychiurus folsomi (Schffer, 1900). Thalassaphorura udagaiensis sp. nov. can be separated easily from the similar species T. encarpata by the presence of 10/011/221110 dorsal S-chaetae and shorter anal spines. Protaphorura sholai sp. nov. is the most similar to the species P. microtica, P. subarctica and P. levantina as having the same number of ventral pseudocelli and parapseudocelli on abdomen; it can be easily distinguished by the position of head chaeta p2 in relation to p1 and p3, shorter M and Sp chaetae on Abd V and longer anal spines. We also sequenced the mitochondrial barcode region of cytochrome oxidase I (658 base pairs) to confirm the species status.

Arab-Indian -530 ß-distal promoter haplotype and sickle/Hb D heterozygosis in Badagas of Nilgiris: is it suggestive of Harappan origin?

Origin and ancestry of the Badagas of Nilgiris, an indigenous community of Nilgiris, Tamil Nadu in India, is a controversial topic. During our massive screening for haemoglobinopathies among Badagas, we identified a rare south Indian haemoglobin variant, the haemoglobin D (Hb D) Punjab (HBB: c.364G>C; rs33946267). The observed Hb D cases were double heterozygous with sickle cell trait (HbSD-Punjab) and manifested symptoms. The beta-globin distal promoter motif (-530 cap site BP1U) sequencing revealed a characteristic heterozygous combination of Arab-Indian sickle haplotype ((AT)9 (T)5) with universally common beta gene haplotype ((AT)7 (T)7). The report is a pioneer one informing the presence of Hb-D Punjab from an indigenous or aboriginal group of southern India. By the above information, we support the theory of unicentric tribal origin and distribution during Harappan civilization.

Simple Nested Allele-Specific approach with penultimate mismatch for precise species and sex identification of tiger and leopard.

Accurate species and sex identification of non-invasive and forensic samples of the tiger and leopard is still confusing when using the allele-specific methods. We designed allele-specific methods with penultimate nucleotide mismatch in a nested manner for the exact identification and double-checking of forensic samples. The mismatch design is a novel concept in species and sex identification, making the allele-specific targeting precise. We developed three sets of markers, a 365 bp outer and a 98 bp inner marker for nested tiger species identification assay, 136 bp leopard specific marker, and carnivore sex identification markers. We validated the method with tissue/blood forensic samples of various felids and herbivorous available in our lab and on known fecal samples from Vandalur Zoo. We also collected 37 scat samples at diverse stages of deterioration from the Mudumalai Tiger Reserve, Tamil Nadu, India. The 365 bp targeted markers resulted in 70.2% (n = 22; 22/37) amplification success, while the 98 bp FAM-labelled marker amplified 89% (n = 33; 33/37) scat samples independently. The 136 bp leopard markers answered four scat samples (11%) unrequited by the tiger specific markers. We evaluated species and the sex identification with these markers in another 190 non-invasive samples provided by the Mudumalai Tiger Reserve authorities. Among which 56.3% (n = 107) of samples were recognized as tiger (64 male and 43 female) and 38.9% (n = 74) as leopard (41 male and 33 female). The method supersedes any other previous methods in this regard by its high accuracy and simplicity.

Habitat suitability model of endangered Latidens salimalii and the probable consequences of global warming.

Salim Ali's fruit bat, Latidens salimalii, is a monotypic endangered fruit bat endemic to Western Ghats (WG) with an ambiguous distribution. The distribution range, habitat suitability, and biology of this species are still uncertain. Endemic species inhabiting the high elevation of  WG like L. salimalii are threatened due to climatic change and seeks urgent management interventions. Hence, we developed a habitat suitability model for L. salimalii using MaxEnt in the current climate condition and projected their distribution for three Representation Concentration Pathway (RCP 4.5, 6.0, and 8.5) climate scenarios of the 2070 time frame. The results show that 9531 km2of habitat in WG is suitable for L. salimalii at present, while all the future scenarios estimates propose complete loss of highly suitable habitat. The significant factors influencing the distribution of L. salimalii are the precipitation of the driest month, tree density, rain in the coldest quarter, canopy height, and altitude. The study pioneers in predicting the suitable habitat and emphasis the need to develop strategies for the long-term conservation of endangered L. salimalii in WG under global warming scenarios.

Refutation of media reports on introduction of the red bellied piranha and potential impacts on aquatic biodiversity in India.

The presence of a new, potentially deadly exotic fish resembling the Red Bellied Piranha, Pygocentrus nattereri was reported in India by print media from various aquatic resources. These reports raised dramatic concerns over public health issues and threats to the aquatic biodiversity of India. Considering the potential severity of the issue and concerns raised by the media, we undertook a study to evaluate the reliability of identification reports of the suspected fish, any relationships to other species of piranha and to address any possible threats to the aquatic biodiversity of India. For this study, samples were collected from most of the major river systems and lakes in India and evaluated for taxonomic identifications of the suspect fish and phylogenetic relationships to other fish species. Our results clearly show that the suspect fish is in fact Piaractus brachypomus, a species commonly referred as "Pacu", and not the red bellied piranha, P. nattereri. Comparisons of both fish do show striking similarities that may account for the misreporting in the media. Furthermore, P. brachypomusas is still an exotic fish, and as such may still have potentially harmful impacts on the native aquatic fauna of India. Quick attention to this issue and the imposition of control measures, including market bans, should be considered to prevent further loss of biodiversity.