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1.
J Neurovirol ; 22(6): 831-839, 2016 12.
Article in English | MEDLINE | ID: mdl-27273075

ABSTRACT

Flaviviruses, including Zika and dengue (DENV), pose a serious global threat to human health. Of the 50+ million humans infected with DENV annually, approximately 1-3 % progress to severe disease manifestations, dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Several factors are suspected to mediate the course of infection and pathogenesis of DENV infection. DHF and DSS are associated with vascular leakage and neurological sequelae. Our hypothesis was that altered astrocyte activation and morphology would alter the dynamics of the extracellular space and hence, neuronal and vascular function. We investigated the mechanisms of neuropathogenesis DENV infection in rhesus macaques. There were decreased numbers of GFAP immunopositive astrocytes per unit area, although those that remained had increased arbor length and complexity. This was combined with structural hypertrophy of white matter astrocytes in the absence of increased vascular leakage. Combined, these studies show how even low-grade infection with DENV induces measurable changes within the parenchyma of infected individuals.


Subject(s)
Astrocytes/pathology , Dengue Virus/pathogenicity , Dengue/pathology , White Matter/pathology , Animals , Astrocytes/metabolism , Biomarkers/metabolism , Dengue/genetics , Dengue/metabolism , Dengue/virology , Dengue Virus/classification , Dengue Virus/genetics , Disease Models, Animal , Gene Expression , Glial Fibrillary Acidic Protein/genetics , Glial Fibrillary Acidic Protein/metabolism , Humans , Hypertrophy , Macaca mulatta , Serogroup , White Matter/metabolism , Zonula Occludens-1 Protein/genetics , Zonula Occludens-1 Protein/metabolism
2.
J Neurovirol ; 20(5): 474-84, 2014 Oct.
Article in English | MEDLINE | ID: mdl-24970236

ABSTRACT

Cortical function is disrupted in neuroinflammatory disorders, including HIV-associated neurocognitive disorders (HAND). Astrocyte dysfunction includes retraction of foot processes from the blood-brain barrier and decreased removal of neurotransmitters from synaptic clefts. Mechanisms of astrocyte activation, including innate immune function and the fine neuroanatomy of astrocytes, however, remain to be investigated. We quantified the number of glial fibrillary acidic protein (GFAP)-labeled astrocytes per square millimeter and the proportion of astrocytes immunopositive for Toll-like receptor 2 (TLR2) to examine innate immune activation in astrocytes. We also performed detailed morphometric analyses of gray and white matter astrocytes in the frontal and parietal lobes of rhesus macaques infected with simian immunodeficiency virus (SIV), both with and without encephalitis, an established model of AIDS neuropathogenesis. Protoplasmic astrocytes (gray matter) and fibrous astrocytes (deep white matter) were imaged, and morphometric features were analyzed using Neurolucida. Gray matter and white matter astrocytes showed no change in cell body size in animals infected with SIV regardless of encephalitic status. In SIV-infected macaques, both gray and white matter astrocytes had shorter, less ramified processes, resulting in decreased cell arbor compared with controls. SIV-infected macaques with encephalitis showed decreases in arbor length in white matter astrocytes and reduced complexity in gray matter astrocytes compared to controls. These results provide the first evidence that innate immune activation of astrocytes is linked to altered cortical astrocyte morphology in SIV/HIV infection. Here, we demonstrate that astrocyte remodeling is correlated with infection. Perturbed neuron-glia signaling may be a driving factor in the development of HAND.


Subject(s)
Astrocytes/immunology , Astrocytes/pathology , Simian Acquired Immunodeficiency Syndrome/immunology , Simian Acquired Immunodeficiency Syndrome/pathology , AIDS Dementia Complex/immunology , AIDS Dementia Complex/pathology , Animals , Disease Models, Animal , Fluorescent Antibody Technique , Macaca mulatta
3.
Am J Pathol ; 184(2): 382-96, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24316110

ABSTRACT

Globoid cell leukodystrophy is a lysosomal storage disease characterized by the loss of galactocerebrosidase. Galactocerebrosidase loss leads to the accumulation of psychosine and subsequent oligodendrocyte cell death, demyelination, macrophage recruitment, and astroglial activation and proliferation. To date, no studies have elucidated the mechanism of glial cell activation and cytokine and chemokine up-regulation and release. We explored a novel explanation for the development of the pathological changes in the early stages of globoid cell leukodystrophy associated with toll-like receptor (TLR) 2 up-regulation in the hindbrain and cerebellum as a response to dying oligodendrocytes. TLR2 up-regulation on microglia/macrophages coincided with morphological changes consistent with activation at 2 and 3 weeks of age. TLR2 up-regulation on activated microglia/macrophages resulted in astrocyte activation and marked up-regulation of cytokines/chemokines. Because oligodendrocyte cell death is an important feature of globoid cell leukodystrophy, we tested the ability of TLR2 reporter cells to respond to oligodendrocyte cell death. These reporter cells responded in vitro to medium conditioned by psychosine-treated oligodendrocytes, indicating the likelihood that oligodendrocytes release a TLR2 ligand during apoptosis. TLRs are a member of the innate immune system and initiate immune and inflammatory events; therefore, the identification of TLR2 as a potential driver in the activation of central nervous system glial activity in globoid cell leukodystrophy may provide important insight into its pathogenesis.


Subject(s)
Immunity, Innate , Leukodystrophy, Globoid Cell/etiology , Leukodystrophy, Globoid Cell/immunology , Aging/metabolism , Animals , Astrocytes/drug effects , Astrocytes/metabolism , Astrocytes/pathology , Calcium-Binding Proteins/metabolism , Cell Aggregation/drug effects , Cell Line , Cell Shape/drug effects , Chemokines/metabolism , Disease Models, Animal , Fluorescence , Glial Fibrillary Acidic Protein/metabolism , Humans , Immunity, Innate/drug effects , Leukodystrophy, Globoid Cell/pathology , Macrophages/drug effects , Macrophages/metabolism , Macrophages/pathology , Mice , Microfilament Proteins/metabolism , Microglia/drug effects , Microglia/metabolism , Microglia/pathology , Myelin Sheath/metabolism , Oligodendroglia/drug effects , Oligodendroglia/metabolism , Oligodendroglia/pathology , Psychosine/pharmacology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rhombencephalon/metabolism , Signal Transduction/drug effects , Toll-Like Receptor 1/metabolism , Toll-Like Receptor 2/genetics , Toll-Like Receptor 2/metabolism , Up-Regulation/drug effects
4.
Article in English | MEDLINE | ID: mdl-24350061

ABSTRACT

Brucella melitensis, a bacterial pathogen and agent of epizootic abortion causes multiple pathologies in humans as well as a number of agriculturally important animal species. Clinical human brucellosis manifests as a non-specific, chronic debilitating disease characterized by undulant fever, arthropathies, cardiomyopathies and neurological sequelae. These symptoms can occur acutely for a few weeks or persist for months to years. Within the brain, endothelial and glial cells can be infected leading to downstream activation events including matrix metalloprotease (MMP) and cytokine secretion and Toll-like receptor (TLR) signaling. These events are likely to lead to tissue remodeling, including morphologic changes in neuronal and glial cells, which are linked to neurological complications including depressive behavior, immune activation and memory loss. Our hypothesis was that B. melitensis infection and neurobrucellosis would lead to activation of astrocytes through upregulation of TLR2 and stimulate concurrent changes in the microanatomy. All six animals were infected via inhalation route. TLR2 expression was approximately doubled in white matter astrocytes of infected rhesus macaques. There was also a 50% increase in the number of astrocytes per unit area in subcortical white matter tracts suggesting increased innate immune activation. This coincided with dramatic increases in the length and complexity of the cell arbor of hypertrophic astrocytes in both cortical gray and white matter. Thus, aerosol-induced brucellosis results in dramatically increased innate immune activation of astrocytes in the absence of widespread neuroinflammation.


Subject(s)
Astrocytes/immunology , Astrocytes/microbiology , Brucella melitensis/immunology , Brucellosis/pathology , Toll-Like Receptor 2/biosynthesis , Aerosols , Animals , Brain/pathology , Brucellosis/microbiology , Disease Models, Animal , Gene Expression , Histocytochemistry , Macaca mulatta , Microscopy, Fluorescence
5.
PLoS One ; 8(7): e69980, 2013.
Article in English | MEDLINE | ID: mdl-23922882

ABSTRACT

BACKGROUND: Self-injurious behavior (SIB) is a complex condition that exhibits a spectrum of abnormal neuropsychological and locomotor behaviors. Mechanisms for neuropathogenesis could include irregular immune activation, host soluble factors, and astrocyte dysfunction. METHODS: We examined the role of astrocytes as modulators of immune function in macaques with SIB. We measured changes in astrocyte morphology and function. Paraffin sections of frontal cortices from rhesus macaques identified with SIB were stained for glial fibrillary acidic protein (GFAP) and Toll-like receptor 2 (TLR2). Morphologic features of astrocytes were determined using computer-assisted camera lucida. RESULTS: There was atrophy of white matter astrocyte cell bodies, decreased arbor length in both white and gray matter astrocytes, and decreased bifurcations and tips on astrocytes in animals with SIB. This was combined with a five-fold increase in the proportion of astrocytes immunopositive for TLR2. CONCLUSIONS: These results provide direct evidence that SIB induces immune activation of astrocytes concomitant with quantifiably different morphology.


Subject(s)
Astrocytes/pathology , Macaca/physiology , Self-Injurious Behavior/immunology , Self-Injurious Behavior/pathology , Animals , Astrocytes/immunology , Atrophy/immunology , Atrophy/pathology , Cell Size , Glial Fibrillary Acidic Protein/analysis , Macaca/immunology , Self-Injurious Behavior/physiopathology , Toll-Like Receptor 2/analysis
6.
J Cell Physiol ; 228(6): 1284-94, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23154943

ABSTRACT

The foot processes of astrocytes cover over 60% of the surface of brain microvascular endothelial cells, regulating tight junction integrity. Retraction of astrocyte foot processes has been postulated to be a key mechanism in pathology. Therefore, movement of an astrocyte in response to a proinflammatory cytokine or even limited retraction of processes would result in leaky junctions between endothelial cells. Astrocytes lie at the gateway to the CNS and are instrumental in controlling leukocyte entry. Cultured astrocytes typically have a polygonal morphology until stimulated. We hypothesized that cultured astrocytes which were induced to stellate would have an activated phenotype compared with polygonal cells. We investigated the activation of astrocytes derived from adult macaques to the cytokine TNF-α under resting and stellated conditions by four parameters: morphology, intermediate filament expression, adhesion, and cytokine secretion. Astrocytes were stellated following transient acidification; resulting in increased expression of GFAP and vimentin. Stellation was accompanied by decreased adhesion that could be recovered with proinflammatory cytokine treatment. Surprisingly, there was decreased secretion of proinflammatory cytokines by stellated astrocytes compared with polygonal cells. These results suggest that astrocytes are capable of multiple phenotypes depending on the stimulus and the order stimuli are applied.


Subject(s)
Astrocytes/metabolism , Cytokines/metabolism , Inflammation Mediators/metabolism , Animals , Astrocytes/drug effects , Astrocytes/immunology , Biomarkers/metabolism , Buffers , Cell Adhesion , Cell Shape , Cells, Cultured , Cytokines/genetics , Female , Gene Expression Regulation , Glial Fibrillary Acidic Protein/metabolism , HEPES/pharmacology , Hydrogen-Ion Concentration , Intermediate Filaments/metabolism , Macaca mulatta , Male , Neural Cell Adhesion Molecules/metabolism , Phenotype , Time Factors , Vimentin/metabolism
7.
J Neurovirol ; 18(3): 213-21, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22535448

ABSTRACT

HIV infection in the brain and the resultant encephalitis affect approximately one third of individuals infected with HIV, regardless of treatment with antiretroviral drugs. Microglia are the resident phagocytic cell type in the brain, serving as a "first responder" to neuroinvasion by pathogens. The early events of the microglial response to productively infected monocyte/macrophages entering the brain can best be investigated using in vitro techniques. We hypothesized that activation of microglia would be specific to the presence of simian immunodeficiency virus (SIV)-infected macrophages as opposed to responses to macrophages in general. Purified microglia were grown and stimulated with control or SIV-infected macrophages. After 6 h, aliquots of the supernatant were analyzed for 23 cytokines using Millipore nonhuman primate-specific kit. In parallel experiments, morphologic changes and cytokine expression by individual microglia were examined by immunofluorescence. Surprisingly, the presence of macrophages was more important to the microglial response rather than whether the macrophages were infected with SIV. None of the cytokines examined were unique to co-incubation with SIV-infected macrophages compared with control macrophages, or their supernatants. Media from SIV-infected macrophages, however, did induce secretion of higher levels of IL-6 and IL-8 than the other treatments. As resident macrophages in the brain, microglia would be expected to have a strong response to infiltrate innate immune cells such as monocyte/macrophages. This response is triggered by incubation with macrophages, irrespective of whether or not they are infected with SIV, indicating a rapid, generalized immune response when infiltrating macrophages entering the brain.


Subject(s)
Cytokines/immunology , Frontal Lobe/immunology , Macrophages/immunology , Microglia/immunology , Simian Acquired Immunodeficiency Syndrome/immunology , Animals , Cell Communication , Cell Movement , Coculture Techniques , Culture Media, Conditioned , Cytokines/biosynthesis , Frontal Lobe/pathology , Frontal Lobe/virology , Macaca mulatta , Macrophages/pathology , Macrophages/virology , Microglia/pathology , Microglia/virology , Primary Cell Culture , Simian Acquired Immunodeficiency Syndrome/pathology , Simian Acquired Immunodeficiency Syndrome/virology , Simian Immunodeficiency Virus/immunology
8.
J Neurosci Methods ; 207(1): 91-6, 2012 May 30.
Article in English | MEDLINE | ID: mdl-22484463

ABSTRACT

The blood-brain barrier (BBB) is increasingly being recognized as a site of special scientific importance. Numerous models of the BBB have been constructed over the past years with increasingly mechanistic studies of fundamental questions of cell biology and neuroimmunology. However, there has been a limiting factor of not being able to perform real-time studies of BBB function utilizing 3D models. Equally, real-time models have been limited mainly to 2D models comprised solely of endothelial cells (ECs). To measure changes in the electrical resistance across a BBB model, when adding inflammatory or stem cells which will interact with co-cultured glial cells has, to date, been beyond the capabilities of models. We have cultured an inverted BBB model with ECs on electrodes which are on the lower surface of xCELLigence Cell Invasion Migration plates. Glial cells were cultured in the basal well with foot processes extending through the filters to make contact with the ECs. SIV-infected macrophages decreased electrical resistance of the EC monolayer when added to the "parenchymal" face of the model. We present a novel inverted blood-brain barrier model that allow real time analyses of endothelial cell adhesion during modeled neuroinflammation.


Subject(s)
Blood-Brain Barrier/metabolism , Cell Culture Techniques/methods , Endothelial Cells/metabolism , Models, Biological , Neuroglia/metabolism , Animals , Blood-Brain Barrier/immunology , Electric Impedance , Endothelial Cells/immunology , Inflammation/immunology , Inflammation/metabolism , Macaca , Macrophages/immunology , Macrophages/virology , Microscopy, Confocal , Neuroglia/immunology , Simian Acquired Immunodeficiency Syndrome/immunology
9.
PLoS One ; 6(6): e21496, 2011.
Article in English | MEDLINE | ID: mdl-21720550

ABSTRACT

Extracellular matrix factors within the tumor microenvironment that control resistance to chemotherapeutics are poorly understood. This study focused on understanding matrix adhesion pathways that control the oral carcinoma response to cisplatin. Our studies revealed that adhesion of HN12 and JHU012 oral carcinomas to carcinoma matrix supported tumor cell proliferation in response to treatment with cisplatin. Proliferation in response to 30 µM cisplatin was not observed in HN12 cells adherent to other purified extracellular matrices such as Matrigel, collagen I, fibronectin or laminin I. Integrin ß1 was important for adhesion to carcinoma matrix to trigger proliferation after treatment with cisplatin. Disruption of talin expression in HN12 cells adherent to carcinoma matrix increased cisplatin induced proliferation. Pharmacological inhibitors were used to determine signaling events required for talin deficiency to regulate cisplatin induced proliferation. Pharmacological inhibition of NF-kB reduced proliferation of talin-deficient HN12 cells treated with 30 µM cisplatin. Nuclear NF-kB activity was assayed in HN12 cells using a luciferase reporter of NF-kB transcriptional activity. Nuclear NF-kB activity was similar in HN12 cells adherent to carcinoma matrix and collagen I when treated with vehicle DMSO. Following treatment with 30 µM cisplatin, NF-kB activity is maintained in cells adherent to carcinoma matrix whereas NF-kB activity is reduced in collagen I adherent cells. Expression of talin was sufficient to trigger proliferation of HN12 cells adherent to collagen I following treatment with 1 and 30 µM cisplatin. Talin overexpression was sufficient to trigger NF-kB activity following treatment with cisplatin in carcinoma matrix adherent HN12 cells in a process disrupted by FAK siRNA. Thus, adhesions within the carcinoma matrix create a matrix environment in which exposure to cisplatin induces proliferation through the function of integrin ß1, talin and FAK pathways that regulate NF-kB nuclear activity.


Subject(s)
Cisplatin/pharmacology , Drug Resistance, Neoplasm/drug effects , Extracellular Matrix/metabolism , Mouth Neoplasms/metabolism , NF-kappa B/metabolism , Talin/metabolism , Cell Line, Tumor , Cell Nucleus/drug effects , Cell Nucleus/metabolism , Cell Proliferation/drug effects , Cell-Matrix Junctions/drug effects , Cell-Matrix Junctions/metabolism , Crk-Associated Substrate Protein/metabolism , Extracellular Matrix/drug effects , Humans , Integrin beta1/metabolism , Intracellular Space/drug effects , Intracellular Space/metabolism , Models, Biological , Mouth Neoplasms/pathology , Signal Transduction/drug effects
10.
Biochem Biophys Res Commun ; 406(2): 171-6, 2011 Mar 11.
Article in English | MEDLINE | ID: mdl-21291860

ABSTRACT

Ligand engagement by integrins induces receptor clustering and formation of complexes at the integrin cytoplasmic face that controls cell signaling and cytoskeletal dynamics critical for adhesion-dependent processes. This study searches for a subset of integrin effectors that coordinates both tumor cell invasion and resistance to the chemotherapeutic drug cisplatin in oral carcinomas. Candidate integrin effectors were identified in a proteomics screen of proteins recruited to clustered integrin αß1, α(v)ß or α(6)ß receptors in oral carcinomas. Proteins with diverse functions including microtubule and actin binding proteins, and factors involved in trafficking, transcription and translation were identified in oral carcinoma integrin complexes. Knockdown of effectors in the oral carcinoma HN12 cells revealed that p130Cas, Dek, Src and talin were required for invasion through Matrigel. Disruption of talin or p130Cas by RNA interference increased resistance to cisplatin, whereas targeting Dek, Src or zyxin reduced HN12 resistance to cisplatin. Analysis of the spreading of HN12 cells on collagen I and laminin I revealed that a decrease in p130Cas or talin expression inhibited spreading on both matrices. Interestingly, a reduction in zyxin expression enhanced spreading on laminin I and inhibited spreading on collagen I. Reduction of Dek, Src, talin or zyxin expression reduced HN12 proliferation by 30%. Proliferation was not affected by a reduction in p130Cas expression. We conclude that p130Cas, Src and talin function in both oral carcinoma invasion and resistance to cisplatin.


Subject(s)
Carcinoma/pathology , Crk-Associated Substrate Protein/metabolism , Drug Resistance, Neoplasm , Mouth Neoplasms/pathology , Talin/metabolism , src-Family Kinases/metabolism , Carcinoma/drug therapy , Carcinoma/metabolism , Cell Line, Tumor , Cell Proliferation , Cisplatin/pharmacology , Collagen/metabolism , Collagen Type I/metabolism , Crk-Associated Substrate Protein/genetics , Drug Combinations , Humans , Integrin alpha1beta1/metabolism , Integrin alpha5beta1/metabolism , Integrin alpha6beta1/metabolism , Laminin/metabolism , Mouth Neoplasms/drug therapy , Mouth Neoplasms/metabolism , Neoplasm Invasiveness , Proteoglycans/metabolism , Proteomics , RNA, Small Interfering/genetics , Talin/genetics , src-Family Kinases/genetics
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