ABSTRACT
Twenty-six trichothecene mycotoxins produced by Fusarium sporotrichioides (MC-72083) and Fusarium sambucinum were screened for relative cytotoxicity in cultured baby hamster kidney (BHK-21) cells. The relative cytotoxicity was measured as LC100. The most cytotoxic trichothecenes were T-2 toxin (5 ng/ml) and the recently isolated 4-propanoyl HT-2 (5 ng/ml) and 3'-hydroxy T-2 toxin (5 ng/ml). T-2 tetraol (1 x 10(4) ng/ml), 8-beta-hydroxytrichothecene (1 x 10(4) ng/ml), sporotrichiol (2 x 10(4) ng/ml), 8-oxodiacetoxyscirpenol (6 x 10(4) ng/ml) and 8-acetyl T-2 tetraol (1 x 10(5) ng/ml) were the least toxic of the regular trichothecenes. None of the modified trichothecenes or the apotrichothecene were very cytotoxic: 8-beta-hydroxysambucoin (2 x 10(3) ng/ml), FS-1 (5 x 10(3) ng/ml), 8-alpha-hydroxysambucoin (8 x 10(4) ng/ml) and trichotriol (1 x 10(5) ng/ml). The modified trichothecenes, FS-2 and FS-3, were not toxic even at 1 x 10(5) ng/ml. The baby hamster kidney cell bioassay proved to be a very sensitive and reproducible means of screening new trichothecene mycotoxins for relative cytotoxicity.
Subject(s)
Fusarium , Trichothecenes/toxicity , Animals , Biological Assay , Cell Line , Cell Survival/drug effects , Molecular Structure , Reproducibility of Results , Trichothecenes/chemistryABSTRACT
From the stem bark of Erythrina eriotriocha, a novel isoflavone, eriotriochin has been isolated and characterized, in addition to known compounds auriculatin, dihydroauriculatin, and 3'-O-methylorobol. The structure of compound 1 was determined by COSY, selective INEPT, 2D HMBC, NOESY, DEPT, and other spectroscopic techniques.
Subject(s)
Erythrina/analysis , Flavonoids/isolation & purification , Isoflavones/isolation & purification , Plants, Medicinal/analysis , Molecular StructureABSTRACT
In addition to the known compounds erythinasinate [1] and abyssinone V [2], two new flavonoids were isolated from the CHCl3 extract of the stem bark of Erythrina eriotriocha and characterized by spectroscopic techniques as 3'-prenylnaringenin [3] and 2'-hydroxy-5'-methoxybiochanin A [4].
Subject(s)
Fabaceae/analysis , Fatty Acids, Monounsaturated/isolation & purification , Flavanones , Flavonoids/isolation & purification , Plants, Medicinal , Fatty Acids, Monounsaturated/analysis , Flavonoids/analysis , Isoflavones , Magnetic Resonance Spectroscopy , Mass SpectrometryABSTRACT
Garcinia epuctata is an indigenous medicinal plant of Cameroon from which taxifolin 6-C-glucoside was isolated and characterized. The nmr assignments obtained from COSY and INAPT experiments are reported for the first time.
