Treatment with an Anti-CK2 Synthetic Peptide Improves Clinical Response in COVID-19 Patients with Pneumonia. A Randomized and Controlled Clinical Trial.

The instrumental role of CK2 in the SARS-CoV-2 infection has pointed out this protein kinase as promising therapeutic target in COVID-19. Anti-SARS-CoV-2 activity has been reported by CK2 inhibitors in vitro; however, no anti-CK2 clinical approach has been investigated in COVID-19. This trial aimed to explore the safety and putative clinical benefit of CIGB-325, an anti-CK2 peptide previously assessed in cancer patients. A monocentric, controlled, and therapeutic exploratory trial of intravenous CIGB-325 in adults hospitalized with COVID-19 was performed. Twenty patients were randomly assigned to receive CIGB-325 (2.5 mg/kg/day during 5-consecutive days) plus standard-of-care (10 patients) or standard-of-care alone (10 patients). Adverse events were classified by the WHO Adverse Reaction Terminology. Parametric and nonparametric statistical analyses were performed according to the type of variable. Considering the small sample size, differences between groups were estimated by Bayesian analysis. CIGB-325 induced transient mild and/or moderate adverse events such as pruritus, flushing, and rash in some patients. Both therapeutic regimens were similar with respect to SARS-CoV-2 clearance in nasopharynx swabs over time. However, CIGB-325 significantly reduced the median number of pulmonary lesions (9.5 to 5.5, p = 0.042) at day 7 and the proportion of patients with such an effect was also higher according to Bayesian analysis (pDif > 0; 0.951). Also, CIGB-325 significantly reduced the CPK (p = 0.007) and LDH (p = 0.028) plasma levels at day 7. Our preliminary findings suggest that this anti-CK2 clinical approach could be combined with standard-of-care in COVID-19 in larger studies.

Scientific Representations at the UPR School of Tropical Medicine. IV: Special Technical Studies.

The essay examines the scientific representations that unfolded and evolved at the University of Puerto Rico School of Tropical Medicine (STM) under the auspices of Columbia University (1926-1949). This article on the STM's scientific endeavors is the fourth in a historical serial collection about the images and evolution of sciences at the institution and it portrays the diagrammatic representations of special technical research aspects and studies (i.e., personnel, epidemiology, methodology, animal studies, biology, field studies, treatment and immunology, and chemotherapy agents). The essay focuses on the emerged scientific representations and on the nature and evolution of sciences at the School, and has been divided into four sections: a) images of science, b) evolution during the first two eras, c) the third and last era unfolding, and d) special technical studies. In this paper the scientific representations have been brought about mainly through the analyses of research publications in external and local venues. The analysis of the STM's scientific evolution has been organized in three distinct historical stages: 1926-31, 1932-40, and 1941-49. These representations open an exploration pathway for a better understanding of the intricate interrelationships between the techné and the episteme horizons of tropical medical science in Puerto Rico.

Scientific Representations at the UPR School of Tropical Medicine. III: The Evolution of Science, the Last Era (1941-1949).

The essay examines the scientific representations that unfolded and evolved at the University of Puerto Rico School of Tropical Medicine (STM) under the auspices of Columbia University (1926-1949). It focuses on the emerged scientific representations and on the nature and evolution of sciences at the School, and has been divided into four sections: images of science, evolution during the first two eras (1926-31 and 1932-40), the unfolding last era (1941-49), and special technical studies. This article on the STM's scientific endeavors is the third in this historical serial collection about the images and evolution of sciences at the institution, and portrays the events and processes of the last scientific era. It analyzes the faculty's principal investigations, development of research programs, and concomitant scientific productivity and research outcomes. The scientific representations have been brought forth through the analyses of different sources: academic and research reports, and publications in external and local venues. The analysis of the STM's scientific evolution has been organized in three distinct chronological stages, while also considering other time evolving models (e.g., historical moments). The main themes of the collection are the scientific images and knowledge exemplars: the emergence of a tradition. An analytical framework of research schemas, exemplars of knowledge, and epistemes proved useful and constructive. These studies on the history of science allow for the postulation of an 'enriched thesis' on the different kinds of paradigmatic diseases of tropical medicine in Puerto Rico during the 20th Century, and enable further substantiation of the tropical obliviousness thesis.

Scientific Representations at the UPR School of Tropical Medicine. I: Images of Science.

The essay examines the scientific representations that prevailed at the University of Puerto Rico School of Tropical Medicine (STM) under the auspices of Columbia University (1926-1949). This article on the STM's scientific endeavors is the first in a historical serial collection about the images and evolution of sciences at the institution. It reviews faculty's principal investigations (by disciplines and research problems), development of research programs, and concomitant scientific productivity and research outcomes. The essay focuses on the emerged scientific representations and the nature of sciences, and has been divided into four sections: 1) images of science, 2) the evolution of science in the first two eras (1926-40), 3) the third and last era unfolding (1941-49), and 4) special studies. This first paper focuses on the scientific images that emerged from an examination of communities' interactions, networks, and academic and foundational documents. The scientific representations have been brought about through the analyses of different sources: academic and research reports, and publications in external and local venues. The most significant findings of this representational inquiry are: the idea of an academic tropical center in the tropics had a shared colonial-metropolis image; the community of common, but unequal, scientific citizens became an integrated epistemological community; interdisciplinary cooperation was the School's research dictum; and an image of a mature science and school of tropical medicine emerged. The richness and varieties of the practices and outcomes of science at the STM are analytically viewed as research schemas, exemplars of knowledge (paradigms), and epistemological fields (epistemes).

Extreme-QTL mapping of monepantel resistance in Haemonchus contortus.

BACKGROUND: Haemonchus contortus, a gastrointestinal nematode parasite of sheep, is mainly controlled by anthelmintics; the occurrence of anthelmintic resistance leads to treatment failures and increases economic burden. Because molecular mechanisms involved in drug resistance can be elucidated by genomic studies, an extreme quantitative trait locus (X-QTL) mapping approach was used to identify co-segregation of the resistance phenotype with genetic markers to detect the genome-wide variants associated with monepantel resistance in H. contortus. METHODS: A cross between H. contortus isolates using parental susceptible (Par-S) males and monepantel resistant (Par-R) females resulted in SR progeny, while reciprocal cross resulted in RS progeny. Pools (n = 30,000) of infective larvae (L3) recovered from Par-R, and from SR and RS populations in the F3 generation, collected both before (unselected group) and 7 days after (selected group) selection with monepantel treatment in sheep hosts, were subjected to genome sequencing (Pool-Seq). Pairwise comparisons of allele frequencies between unselected and selected groups were performed for each population by Fisher's exact test (FET) and for both populations combined by a Cochran-Mantel-Haenszel (CMH) test. RESULTS: Mapping rates varied from 80.29 to 81.77% at a 90.4X mean coverage of aligned reads. After correction for multiple testing, significant (P < 0.05) changes in allele frequencies were detected by FET for 6 and 57 single nucleotide polymorphisms (SNPs) in the SR and RS populations, respectively, and by the CMH test for 124 SNPs in both populations. The significant variants located on chromosome 2 generated a selection signal in a genomic region harboring the mptl-1, deg-3 and des-2 genes, previously reported as candidates for monepantel resistance. In addition, three new variants were identified in the mptl-1 gene. CONCLUSIONS: This study expands knowledge on genome-wide molecular events underlying H. contortus resistance to monepantel. The identification of a genome region harboring major genes previously associated with monepantel resistance supports the results of the employed X-QTL approach. In addition, a deletion in exon 11 of the mptl-1 gene should be further investigated as the putative causal mutation leading to monepantel resistance.

Scientific Representations at the UPR School of Tropical Medicine. II: The Evolution of Science, the First Two Eras (1926-31, 1932-1940).

The essay examines the scientific representations that unfolded and manifested at the University of Puerto Rico School of Tropical Medicine (STM) under the auspices of Columbia University (1926-1949). It focuses on the emerged scientific representations and on the kind of science practiced at the School, and has been divided into four sections: images of science, evolution during the first two eras (1926-31 and 1932-40), the unfolding third and last era (1941-49), and special technical studies. This article on the STM's scientific endeavors is the second in this historical serial collection about the images and evolution of sciences at the institution, and portrays the events and processes of the first two scientific eras. It reviews the faculty's principal investigations, development of research programs, and concomitant scientific productivity and research outcomes. The following historical sources were considered: academic and research reports, and publications in external and local venues. On findings, bacteriological investigations and studies on mycology and dermatological fungal infections characterized research during the first era. Parasitology became the hegemonic science of tropical medicine during the second scientific era, in conjunction with important studies on nutrition and streptococcal bacteriological infections. Variations of an earlier tropical medicine discourse of 'abundance of material for study' were: the socioeconomic toll of tropical diseases and a biopower exertion of induced recruitment of medical bodies. And public health field-community studies became a critical research approach at-end of periods. The evolution of science in the last and third era will be the main subject of the next article.

Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep / Relação entre genes de fatores de virulência em Staphylococcus spp. coagulase-negativos e a falha do tratamento antimicrobiano da mastite subclínica ovina

Coagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of β-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep.(AU)

Staphylococus spp. coagulase-negativos (SCN) estão entre os principais micro-organismos envolvidos na mastite ovina. O tratamento ao final da lactação pode contribuir com a cura e a prevenção de casos subclínicos durante a lactação seguinte. Todavia, fatores de virulência e mecanismos de resistência apresentados por SCN podem reduzir as taxas de cura. Os objetivos desse estudo foram identificar as espécies de SCN no leite de ovelhas com mastite com e sem tratamento antimicrobiano e investigar a presença de genes relacionados com resistência a antibióticos beta lactâmicos, formação de biofilmes, produção de enterotoxinas e produção da toxina da síndrome do choque tóxico. Casos de falhas no tratamento foram relacionados com a presença/ausência dos respectivos genes. Sessenta ovelhas foram divididas em três grupos: G1, sem tratamento; G2, animais tratados via intramamária com 100mg de cloxacilina antes da secagem; e G3, ovelhas tratadas via intramamária com 50 mg de cloxacilina nanoparticulada. Amostras de leite foram obtidas durante a secagem e 15 e 30 dias depois do parto na lactação seguinte. As análises para identificar as espécies de SCN foram conduzidas por meio da técnica de Internal transcribe spacer e a investigação dos genes responsáveis pelos fatores de virulência e resistência à oxacilina foi realizada usando a técnica reação em cadeia da polimerase. Nenhuma amostra foi positiva para o gene mecA. O único gene relacionado com a produção de enterotoxinas foi o sec. Dentre os genes relacionados com a produção de biofilme, icaD foi o único identificado nos três grupos experimentais. Staphylococcus warneri foi a principal espécie de SCN isolada durante o pré e pós-parto. As espécies que apresentaram genes relacionados com a produção de enterotoxinas e biofilmes estavam presentes nas ovelhas não curadas.(AU)

The Puerto Rico Journal of Public Health and Tropical Medicine (1925-1950): From a Health Department Bulletin to a UPR School of Tropical Medicine Scientific Journal.

This essay presents a history of the scientific journal of the University of Puerto Rico, School of Tropical Medicine (STM) under the auspices of Columbia University: The Puerto Rico Journal of Public Health and Tropical Medicine. This is the third article in a historical series about the STM, and includes supporting information relevant to the forthcoming articles on the school's scientific endeavors. This article is conceived as a history from the perspective of the literature of journal genre in the field of tropical medicine. The STM scientific journal, precursor of the Puerto Rico Health Sciences Journal, had five main stages. First (1925-1927), originated as an official bulletin of the Health Department (Porto Rico Health Review). Second (1927-1929), became a project of mutual collaboration between the Health Department and the STM, and the publication's title reflected the fields of public health and tropical medicine. Third (1929-1932), acquired a scientific focus as it changed to a quarterly science publication. Fourth (1932-1942), became a fully bilingual journal and acquired its definitive name. Fifth (1942-1950), the final phase in which the first Puerto Rican Director became the principal editor until the Journal's dissolution. The analysis of authorship and the content analysis of the topics of diseases, public health and basic sciences, clarify the history of tropical medicine during the first half of the 20th century in Puerto Rico. The article highlights major symbolic events that delve into the understanding of a collaborative exemplar of the modernity of medical science.

Influence of lactation stages and rain periods on subclinical mastitis in meat producing ewes / Influência dos estágios de lactação e ocorrência de chuvas sobre a mastite subclínica em ovelhas com aptidão para produção de carne

ABSTRACT: Mastitis negatively influences the survival and weight gain of ovines for meat production. The purpose of this study was to investigate, in sheep for meat production, the occurrence of subclinical mastitis in ewes at the end of lactation and beginning of the consecutive lactation and to assess the composition and cellular characteristics of milk as a function of different rainfall indices. Mammary halves (821) of Santa Ines (479) and Morada Nova (342) ewes were examined. Milk samples were collected in two different moments of lactation: at weaning and postpartum of the consecutive lactation. Sample collection periods were called "dry" or "rainy" according to the rainfall index in the month immediately before the month of collection. The occurrence of subclinical mastitis at weaning in the Santa Ines and Morada Nova ewes were 16.4 and 12.6% in the dry period, and 17.7 and 23.5% in the rainy period, respectively. In the consecutive lactation period, the occurrences were 26.7 and 27.7% in the dry period and 41.8 and 39.1% in the rainy period, for the Santa Ines and Morada Nova ewes, respectively. Postpartum stage was critical for the occurrence of subclinical mastitis, as compared to that at the end of the previous lactation. Occurrence of the disease negatively influenced the SCC in the milk at the beginning of lactation and changed its composition, mainly in the rainiest periods, probably due to a difficulty in maintaining hygiene in the environment where the animals remained.

RESUMO: A mastite influencia negativamente na sobrevivência e no ganho de peso em ovinos com aptidão para produção de carne. O objetivo do presente estudo foi investigar, em ovelhas para produção de carne, a ocorrência de mastite ovina subclínica, no final de lactação e no início da lactação consecutiva, bem como avaliar as características composicionais e celulares do leite em função de diferentes índices pluviométricos. Metades mamárias (821) das raças 'Santa Inês' (479) e 'Morada Nova' (342) foram analisadas. As amostras de leite foram colhidas em dois estágios diferentes de lactação: no desmame e após o parto da lactação consecutiva. Os períodos de colheita de amostras foram denominados "seco" ou "chuvoso", conforme o índice de chuva no mês imediatamente antes ao mês da colheita. A ocorrência de mastite subclínica na desmama nas raças 'Santa Inês' e 'Morada Nova' foi de 16,4 e 12,6% no período seco, e 17,7 e 23,5% no período chuvoso, respectivamente. Na lactação consecutiva, as ocorrências foram 26,7 e 27,7% no período seco e 41,8 e 39,1% no período chuvoso, nas ovelhas 'Santa Inês' e 'Morada Nova', respectivamente. O período pós-parto foi crítico para a ocorrência de mastite subclínica, comparado àquele no final da lactação anterior. A ocorrência da doença teve uma influência negativa na CCS do leite no início da lactação e alterou sua composição, principalmente nos períodos mais chuvosos, provavelmente por dificuldade em manter a higiene no ambiente onde os animais permaneceram.

Uso de antimicrobiano nanoparticulado para o tratamento da mastite subclínica de ovelhas de corte no período seco / Use of nanoparticulated antimicrobians to treat subclinical mastitis of ewes during the dry period

A inflamação da glândula mamária é uma das principais causas de prejuízo na ovinocultura. Este estudo teve como objetivo investigar as taxas de cura do tratamento da mastite subclínica após infusão intramamária de princípio ativo antimicrobiano no momento da secagem, em formulações convencional e nanoparticulada. Os rebanhos estavam localizados em São Carlos, São Paulo, Brasil. Analisou-se um total de 584 glândulas mamárias de 307 ovelhas de aptidão para produção de carne. Triagem prévia dos casos subclínicos de mastite foi efetuada por meio do California Mastitis Test (CMT) e/ou da contagem de células somáticas (CCS). Análises microbiológicas foram realizadas para confirmação da etiologia infecciosa. As glândulas mamárias com mastite subclínica foram distribuídas em três grupos: G1 (Controle; glândulas mamárias que não receberam tratamento antimicrobiano); G2 (glândulas mamárias em que foi administrado 100 mg de cloxacilina benzatina em estrutura convencional) e G3 (glândulas mamárias em que foi administrado 50 mg de cloxacilina benzatina em estrutura nanoencapsulada). O tratamento aplicado ao G3 mostrou-se mais eficiente (P=0,047) na cura de glândulas mamárias com mastite subclínica. O uso da cloxacilina nanoencapsulada no momento da secagem de ovelhas de corte auxilia no controle da mastite subclínica infecciosa e reduz os prejuízos consequentes.(AU)

Inflammation of the mammary gland is one of the main causes of losses in sheep-rearing. This study aimed to investigate the cure rates from treating subclinical mastitis after intramammary infusion of active antimicrobial agents as conventional formulations or as nanoparticles, at the time when the ewes are being dried off. A total of 584 mammary glands in 307 ewes in meat-producing herds located in São Carlos, São Paulo, Brazil, were analyzed. Prescreening of subclinical mastitis cases was done using the California mastitis test (CMT) and/or the somatic cell count (SCC). Microbiological analyses were performed to confirm the infectious etiology. The mammary glands with subclinical mastitis were distributed into three groups: G1 (control; mammary glands that did not receive any antimicrobial treatment); G2 (mammary glands to which 100mg of benzathine cloxacillin in conventional form were administered); and G3 (mammary glands to which 50mg of benzathine cloxacillin in nanoparticulate form were administered). The treatment applied to G3 was more efficient (P=0.047) in curing mammary glands with subclinical mastitis. Use of cloxacillin nanoparticles at the time when the ewes are being dried off helps to control infectious subclinical mastitis and reduces consequential losses among meat-producing herds.(AU)

Marvels and Shadows: Science and Education at the University of Puerto Rico School of Tropical Medicine under the Auspices of Columbia University: An Introduction.

This essay introduces a series of five historical articles on the scientific and educational contributions of the University of Puerto Rico School of Tropical Medicine (STM), under the auspices of Columbia University (1926-1949), to the fields of tropical medicine and public health. The articles will appear in several consecutive issues, and will address various themes as follows: 1) historical antecedents of the STM, particularly institutional precedents; 2) the educational legacy of the STM; 3) a history of the STM scientific journal ("The Puerto Rico Journal of Public Health and Tropical Medicine"); 4) the scientific practices and representations that prevailed at the institution; and, 5) a brief sociocultural history of malaria in Puerto Rico, mainly from the perspective of the STM's scientific and public health activities. The authors have systematically and comprehensively studied a wide variety of documents from different sources based on multiple archives in Puerto Rico, the United States and England. The authors treat the fluid meanings of the examined historical encounters from a research perspective that privilege complex reciprocal interactions, multiple adaptations and elaborate sociocultural constructs present in a collaborative exemplar of the modernity of medical science in a neocolonial tropical context.

The Historical Antecedents of the UPR School of Tropical Medicine.

This article deals with the historical antecedents of the University of Puerto Rico (UPR) School of Tropical Medicine (STM) under the auspices of Columbia University. It presents a general view of the social, institutional and conceptual factors that were correlated with the establishment of the STM. The authors start by examining the historical continuities and discontinuities present during the imperial transitions between Spanish colonial and U.S. military medicine at the turn of the 20th century. The clarification of these changes is important for the proper understanding of the emergence of tropical medicine in Puerto Rico, marked by the identification of the biological determinant of the so called "peasants' anemia." The essay focuses on two institutional precursor events: the Puerto Rico Anemia Commissions (1904-1908) and the Institute of Tropical Medicine and Hygiene (1912-1914). Their nature and work paved the way for the establishment of the STM. The notions of tropical medicine and diseases are considered as historical concepts. The support of the Rockefeller Foundation to several significant public health activities in Puerto Rico is also examined. Finally, the social and health conditions which prevailed at the time of the creation of the STM have been summarized. In general, the article provides a sense of historical context deemed essential to understand the emergence and evolution of the STM.

Diagnosis of subclinical mastitis in Santa Inês and Morada Nova sheep in southeastern Brazil.

The objective of this study was to evaluate different screening limits for the California mastitis test (CMT) and the somatic cell count (SCC) in previous diagnoses of subclinical mastitis in Santa Inês and Morada Nova ewes, which were reared under the same management conditions. Additionally, cutoff points were defined for SCC in accordance with the sensitivity and specificity of the test. A total of 907 mammary halves were subjected to CMT and SCC. The disease was confirmed by means of microbiological identification. Coagulase-negative staphylococci (CNS) were the microorganisms with highest occurrence. The CMT score of 1+ provided adequate sensitivity and specificity at all periods of lactation investigated. This score showed good agreement with SCC, >400,000 cells mL(-1). Higher cell counts favored higher diagnostic specificity. They can be used when producers have financial difficulties relating to treatment or culling of sheep with subclinical mastitis. However, producers should be warned about the risk of false-negative results in the flock.

Efficacy of 11 Brazilian essential oils on lethality of the cattle tick Rhipicephalus (Boophilus) microplus.

Herbal extracts have been investigated as an alternative for parasite control, aiming to slow the development of resistance and to obtain low-cost biodegradable parasiticides. The goal of this study was to evaluate the efficacy, in vitro, of 11 essential oils from Brazil on reproductive efficiency and lethality of the cattle tick Rhipicephalus (Boophilus) microplus. The effects of oils extracted from Curcuma longa, Zingiber officinale, Lippia alba, Lippia gracilis, Lippia origanoides, Lippia sidoides, Mentha arvensis, Mentha piperita, Croton cajucara (white and red), and Croton sacaquinha on ticks were investigated by the Immersion Test with Engorged Females (ITEF) and the modified Larval Packet Test (LPT). Distilled water and 2% Tween 80 were used as control treatments. Chemical analysis of the oils was done with gas chromatography coupled with mass spectrometry. Analysis of the in vitro tests using Probit (SAS program) allowed the calculation of lethal concentrations (LCs). Lower reproductive efficiency indexes and higher efficacy percentages in the ITEF were obtained with the oils extracted from C. longa (24 and 71%, respectively) and M. arvensis oils (27 and 73%, respectively). Lower LC50 was reached with C. longa (10.24 mg/mL), L. alba (10.78 mg/mL), M. arvensis (22.31 mg/mL), L. sidoides (27.67 mg/mL), and C. sacaquinha (29.88 mg/mL) oils. In the LPT, species from Zingiberaceae and Verbenaceae families caused 100% lethality at 25 mg/mL, except for L. sidoides. The most effective oils were from C. longa, L. gracilis, L. origanoides, L. alba, and Z. officinale. The LC50 and LC90 were, respectively: 0.54 and 1.80 mg/mL, 3.21 and 7.03 mg/mL, 3.10 and 8.44 mg/mL, 5.85 and 11.14 mg/mL, and 7.75 and 13.62 mg/mL. The efficacy was directly related to the major components in each essential oil, and the oils derived from Croton genus presented the worst performance, suggesting the absence of synergistic effect among its compounds. Since C. longa, containing 62% turmerone, was the one most efficient against ticks, this compound may be potentially used for tick control, but further research is needed, especially to assess toxicity of these compounds to the host. These new studies, together with the results presented here, may provide a strong rationale for designing pre-clinical and clinical studies with these agents.

Elaboración de un guiso liofilizado para montañistas / Manufacture of a freeze dried stew for mountaineers

Durante la práctica del montañismo, es un hábito ingerir una cena que proporcione gran parte de las necesidades nutricionales y cumpla con algunos requisitos que faciliten su elección. Entre estos se pueden mencionar el tener una composición adecuada, que ocupe volumen y sea liviano, que se conserve bien por un período prolongado de tiempo, que sea de preparación fácil y rápida y que sea bien aceptado por el grupo. Considerando estos requisitos, se elaboró un guiso típico y de consumo habitual en Chile a base de carne y verduras, denominado "charquicán", deshidratado por liofilización con fin de conservar sus caracteristicas nutricionales sensoriales. El producto final está constituido por porciones individuales de 60 g, en envasado al vacío en un envase primario de poliamida-polietileno coextruido, que lo protege de la humedad y del oxígeno atmosférico. Se utilizó además un envase secundario de poliester metalizado para protegerlo de la luz. Los controles de producto optimizado indicaron 6,7 por ciento de humedad y un valor aw de 0.26. La composición química es nutricionamente adecuada, aportando 45,6 por ciento de carbohidratos, 5 por ciento de lípidos y 29,5 por ciento de proteínas. Presentó una calidad sensorial muy buena (puntaje 7,5 en escala de 1 a 9 de Karlsruhe) y una calidad microbiológica buena, ya que cumple con las especificaciones reglamentarias para productos afines, estando por debajo de los límites propuestos para recuento total y hongos y levaduras. El estudio de vida útil se realizó almacenando el producto terminado en condiciones ambientales (20-25ºC y 60-65ºC de HR) controlando simultáneamente la calidad microbiológica y la sensorial. No se observaron diferencias significativas en la calidad microbiológica durante los 200 días controlados. La calidad sensorial en cambio presentó una disminución leve y paulatina hasta alcanzar un puntaje promedio de 6,7 a los 200 días. La aceptación con deportistas de alta montaña en jornadas de escalamiento mostró un escelente aceptación y la buena funcionalidad del producto

Desarrollo y optimización de un jugo isotónico para deportistas / Development and optimization of an isotonic beverage for athletes

Dentro de una línea de investigación de alimentos para deportistas se abordó el diseño, elaboración y control de bebidas isotónicas elaboradas a partir de concentrado natural de manzana. Se establecieron las condiciones de dilución del concentrado y diferentes combinaciones de sales que aportan los electrolitos que se eliminan por la transpiración y que es necesario reponer, como sodio, potacio, cloruro, magnesio y calcio en concentraciones que proporcionen una presión osmótica similar a la de la sangre. Se ensayó además la adición de una premezcla de vitaminas (B1, B2, B6, B12, C, E, ácido fólico, niacina, ácido pantoténico y biotina) en diferentes concentraciones que representaban el 100, 75, 62.5, 60, y 30 por ciento de la Ingesta Diaria Recomendada (IDR). Se seleccionó la adición de un 30 por ciento de la IDR, adicionando sólo aquellas vitaminas de mayor importancia para la actividad física (B1, B2, B6, y C). La calidad de las formulaciones, se optimizó por medio de la evaluación sensorial, considerando como variables las concentraciones del aromatizante, del colorante y de la maltodextrina. En el jugo elaborado se controló pH, acidez, sólidos totales (ºBx), densidad relativa y relación sólidos solubles - acidez, de acuerdo a las normas correspondientes. Además se controló la composición química, el valor calórico, y la calidad microbiológica y sensorial. El jugo elaborado se envasó en envases autosoportantes tipo Doy Pack, de plástico laminado coextruído, en volúmenes de 250 ml. Este producto presentó una calidad microbiológica óptima, buenas características organolépticas y un valor calórico de 101.18 Kcal/250 ml de producto. Al realizar el estudio de vida útil a temperatura ambiente (15-25ºC) y de refrigeración (3-5ºC), el factor limitante fue el sabor, definiéndose una vida útil de por lo menos 3 meses almacenado a 3-5ºC. El producto desarrollado proporcioana las concentaciones de electrolitos necesarias para caracterizarlo como bebida isotómica, recomendada para deportistas y otras actividades que se caracterizan por transpiración intensa

Desarrollo de un confite hiperproteico a base de lupino para deportistas / Development of an hyperproteic lupin-based candy bars for sportmen

Con el fin de implementar la ingesta proteica recomendada para deportistas que necesitan desarrollar mayor masa muscular, se desarrolló un confite constituido por carbohidratos, sólidos, lácteos, aislado proteico de lupino dulce: lupinus albus cv. multolupa con 87 por ciento de proteínas, lupino tostado molido, avena tostada, esencias, preservantes y antioxidantes. Para proteger los confites y mejorar su presentación y aceptabilidad se usó una cobertura de chocolate como recubrimiento. Se realizaron controles sensoriales, microbiológicos, físicos y químicos en los productos optimizados, determinando buena calidad sensorial y microbiológica. La composición química de este confite indica un aporte de 17,4 por ciento de proteínas, 9,2 por ciento de lípidos y 56,8 por ciento de carbohidratos. Su aporte calórico es de 387 kcal/100 g. Se estudió la vida útil en condiciones ambientales 20-25ªC y 55-60 por ciento HR del producto envasado en papel aluminio y con que la calidad se mantiene sin variaciones significativas durante por lo menos 60 días

Lípidos y lipoproteínas plasmáticos en mujeres entrenadas después de una competencia de triatlón / Plasmatic lipids and lipoprotein in trained women after a triathlon competition

El objetivo de este estudio fue determinar los efectos inmediatos y tardíos de una competencia de triatlón sobre lípidos y lipoproteínas plasmáticos en mujeres entrenadas. Fueron estudiadas 8 triatletas mujeres. Todas compitieron en una triatlón internacional que consistió en 1,9 km. de natación, 90 km. de ciclismo y 21 km. de carrera. Se obtuvieron muestras de plasma venoso 24 horas antes de la triatlón y a los 10 minutos, 24 horas , 48 horas y 72 horas después. Los análisis incluyeron la determinación de triglicéridos TG, glicerol G, colesterol HDL C-HDL, colesterol LDL LDL-C y colesterol total CT. No se encontraron cambios significativos inmediatos en los TG. El G plasmático aumentó alos 10 minutos después de la triatlón 65,8 a 177,6 u mol/L; p<0,006, mientras que el C-LDL disminuyó 126 a 77 mg/d L; p<0,05. Además se obtuvo un aumento tardío en los TG a las 72 horas después de la competencia 51,6 mg/dl a 70,3 mg/dl; p<0,05. Finalmente se encontró una disminución significatica en CT 196,9 a 159 mg/dl; p<0,04 a las 24 horas después de la triatlón. Estos datos demuestran que la competencia de triatlón induce modificaciones inmediatas y tardías en lípidos y lipoproteínas plasmáticos en mujeres altamente entrenadas