Early Effects of Mycorrhizal Fungal Inoculum and Fertilizer on Morphological and Physiological Variables of Nursery-Grown Nothofagus alessandrii Plants.

Nothofagus alessandrii (ruil) is an endangered relict species, endemic to the Mediterranean area of Chile, and one of the most threatened trees in the country. Its natural distribution area has been greatly reduced by the effect of human activities; the remaining fragments are mostly intervened and highly deteriorated as a habitat and refuge for the associated biodiversity. In order to produce healthy and resistant nursery plants for recovery and restoration of N. alessandrii forests, this study evaluates the early effects of mycorrhizal fungal inoculum (MFI) combined with fertilization on the cultivation of seedlings. The experiment was established under a completely randomized design with a factorial arrangement of the mycorrhizal factors (M0 = without mycorrhizal, M1 = Thelephora sp. and M2 = Hebeloma sp.) and fertilization (F1 = standard fertilization and F2 = intensive fertilization), with three replicates of each combination, for each type of plant (P1 = plants from one season and P2 = plants from two seasons). Each experimental unit corresponded to a group of 20 plants, with 720 plants in the test. The results indicate that application of fertilizer and MFI significantly affects some growth and photosynthesis parameters of ruil plants in one and two seasons. The morphological parameters obtained in the study show shoot height values ranging between 67 and 91 cm for P1 and between 96 and 111 cm for P2; while, for shoot diameter, values ranged between 7.91 and 8.24 mm for P1 and between 10.91 and 11.49 mm for P2. Although formation of fully developed mycorrhizal roots was not observed during the assay period, we conclude that inoculation of mycorrhizal fungi combined with fertilization could be an efficient strategy to produce a quality plant, in addition to maintaining a high photosynthetic capacity and, therefore, a higher percentage of survival in the field.

Improvement in the physiological and biochemical performance of strawberries under drought stress through symbiosis with Antarctic fungal endophytes.

Strawberry is one of the most widely consumed fruit, but this crop is highly susceptible to drought, a condition strongly associated with climate change, causing economic losses due to the lower product quality. In this context, plant root-associated fungi emerge as a new and novel strategy to improve crop performance under water-deficiency stress. This study aimed to investigate the supplementation of two Antarctic vascular plant-associated fungal endophytes, Penicillium brevicompactum and Penicillium chrysogenum, in strawberry plants to develop an efficient, effective, and ecologically sustainable approach for the improvement of plant performance under drought stress. The symbiotic association of fungal endophytes with strawberry roots resulted in a greater shoot and root biomass production, higher fruit number, and an enhanced plant survival rate under water-limiting conditions. Inoculation with fungal endophytes provokes higher photosynthetic efficiency, lower lipid peroxidation, a modulation in antioxidant enzymatic activity, and increased proline content in strawberry plants under drought stress. In conclusion, promoting beneficial symbiosis between plants and endophytes can be an eco-friendly strategy to cope with drought and help to mitigate the impact of diverse negative effects of climate change on crop production.

Individualization of Pinus radiata Canopy from 3D UAV Dense Point Clouds Using Color Vegetation Indices.

The location of trees and the individualization of their canopies are important parameters to estimate diameter, height, and biomass, among other variables. The very high spatial resolution of UAV imagery supports these processes. A dense 3D point cloud is generated from RGB UAV images, which is used to obtain a digital elevation model (DEM). From this DEM, a canopy height model (CHM) is derived for individual tree identification. Although the results are satisfactory, the quality of this detection is reduced if the working area has a high density of vegetation. The objective of this study was to evaluate the use of color vegetation indices (CVI) in canopy individualization processes of Pinus radiata. UAV flights were carried out, and a 3D dense point cloud and an orthomosaic were obtained. Then, a CVI was applied to 3D point cloud to differentiate between vegetation and nonvegetation classes to obtain a DEM and a CHM. Subsequently, an automatic crown identification procedure was applied to the CHM. The results were evaluated by contrasting them with results of manual individual tree identification on the UAV orthomosaic and those obtained by applying a progressive triangulated irregular network to the 3D point cloud. The results obtained indicate that the color information of 3D point clouds is an alternative to support individualizing trees under conditions of high-density vegetation.

Effect of Temperature on the Germination of Five Coastal Provenances of Nothofagus glauca (Phil.) Krasser, the Most Representative Species of the Mediterranean Forests of South America.

Temperature is one of the most important abiotic factors affecting seed germination, and it is strongly influenced by local site conditions. Seeds of Nothofagus glauca, an endemic and vulnerable species of the Mediterranean region of Chile and the most representative of the Mediterranean forests of South America, were collected. In this study, we evaluated the effect of temperature on different germinative attributes of five N. glauca provenances representative of their natural distribution. The seeds were treated at a constant temperature (i.e., 18 °C, 22 °C, 26 °C, or 30 °C) in the absence of light for 40 days. The results show that in all the provenances, the germination ratio and energy increase linearly with temperature until reaching an optimum temperature (i.e., 22 °C), above which they decrease severely. At 22 °C, the response of average germination speed and germination vigor was significantly higher than with the other temperatures (performance of germination start day was not clear). The base temperature was around 18 °C and the maximum, above 30 °C, which may be close to thermo-inhibition. Given the threat of climate change, it is necessary to increase research in terms of the possible adaptation of this species to increased temperatures and prolonged periods of drought.

Caracterización del material forestal de reproducción de cinco procedencias de nothofagus alessandrii espinosa, una especie en peligro de extinción / Characterization of forest reproductive material from five provenances of nothofagus alessandrii espinosa, an endangered species / Caracterização do material florestal de reprodução de cinco procedências de nothofagus alessandrii espinosa, uma espécie em perigo de extinção

Nothofagus alessandrii es una especie endémica de la zona mesomórfica de Chile que se encuentra en peligro de extinción y de la cual existe aún información insuficiente respecto de las diferencias entre sus poblaciones. Por ello, en este trabajo se analizan y caracterizan parámetros cualitativos y cuantitativos del material germinativo y el comportamiento en vivero de cinco procedencias representativas del área de distribución natural de la especie. Se colectaron semillas de estas procedencias en febrero 2000 y se determinó su tamaño, forma, peso y capacidad germinativa. En septiembre del mismo año se cultivaron plantas en vivero y se evaluó su crecimiento diametral y en altura. Los resultados indican que el tamaño de las semillas es, en general, homogéneo y solo se observan diferencias estadísticas en la longitud de las dímeras y ancho de las trímeras. El peso de 1000 semillas y la capacidad germinativa varió significativamente entre las procedencias. El desarrollo de las plantas en vivero fue homogéneo y no hubo diferencias en el diámetro de cuello de la raíz ni en la altura que alcanzaron las plantas después de una temporada de cultivo. N. alessandrii tiene un comportamiento diferente al de otras especies del mismo género, sin que se observe una variación de tipo clinal. Sin embargo, la procedencia más austral tiende a diferenciarse de las otras y, probablemente, corresponda a un ecotipo diferente.

Nothofagus alessandrii is an endemic and endangered species from the mesomorphic zone of Chile. Currently, differences across populations are not well known. To this end, quantitative and qualitative parameters pertaining to germinative material and nursery performance of seedlings from five provenances representing the natural distribution of the species were analyzed and characterized. Seeds were collected in February 2000 and size, form, weight, and germination capacity were determined. Seedlings were then cultivated in nursery in September of the same year, and basal diameter and height growth were quantified. Results indicate that seed size is generally homogeneous across sites, and statistical differences were limited to length of dimerous seeds and width of trimerous seeds. Seed weight and germination capacity varied significantly among the provenances. After one cultivation season, the development of the nursery plants was homogenous across provenances, with no differences in basal diameter or height. N. alessandrii differs, in relation to the variation among the studied provenances, from that of other species of the same genus, with the exception of a pronounced clinal type. However, the southern-most provenance tended, in general, to differ from the other ones and likely represents a different ecotype.

Nothofagus alessandrii é uma espécie endêmica da zona mesomórfica do Chile que se encontra em perigo de extinção e da qual existe ainda informação insuficiente em relação às diferenças entre suas populações. Por isto, neste trabalho se analisam e caracterizam parâmetros qualitativos e quantitativos do material germinativo e o comportamento em viveiro de cinco procedências representativas da área de distribuição natural da espécie. Recolheram-se sementes destas procedências em fevereiro de 2000 e se determinou seu tamanho, forma, peso e capacidade germinativa. Em setembro do mesmo ano se cultivaram plantas em viveiro e se avaliou seu crescimento diametral e em altura. Os resultados indicam que o tamanho das sementes é, em geral, homogêneo e somente se observam diferenças estatísticas no comprimento das dímeras e largura das trímeras. O peso de 1.000 sementes e a capacidade germinativa variou significativamente em relação às procedências. O desenvolvimento das plantas em viveiro foi homogêneo e não houve diferenças no diâmetro do colo da raíz nem na altura que alcançaram as plantas depois de uma temporada de cultivo. N. alessandrii tem um comportamento diferente ao de outras espécies do mesmo gênero, sem que se observe uma variação do tipo clinal. No entanto, a procedência mais austral tende a diferenciar-se das outras e, provavelmente, corresponda a um ecótipo diferente.