Phlebotominae in peri-domestic and forest environments inhabited by Alouatta caraya in northeastern Argentina.

Multiple species of Phlebotominae are vectors of Leishmania (Protozoa: Trypanosomatidae), which causes visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). To describe the Phlebotominae (Diptera: Psychodidae) related to the environments of black and gold howler monkeys Alouatta caraya (Humbodlt, 1812) (Primates: Atelidae), potential vectors were sampled in different landscapes and vertical strata of sleeping trees. Phlebotomine captured between December 2011 and March 2012 (2365 individuals) belonged to eight species, of which Nyssomyia neivai (Pinto, 1926) (61.4%) and Migonemyia migonei (França, 1920) (18.73%) were the most abundant, and Ny. withmani was recorded for the first time in the Chaco province. In the 'peri-domestic' landscape, the phlebotomine were mainly captured in henhouses (78.7%), whereas the tree canopy in 'rural' and 'wild' landscapes yielded 31.2% and 29.1% of the phlebotomine, respectively. A significant association between the type of landscape and the species of phlebotomine was observed by multivariate analysis. Lutzomyia longipalpis (Lutz & Neiva, 1912) and Mg. migonei were associated with 'peri-domestic' landscape, and Ny. neivai was associated with the 'wild' landscape. The results of this prospective study suggest that the interaction between phlebotomine and A. caraya could be a key factor with respect to understanding the epidemiology of leishmaniasis.

Spatiotemporal dynamics of Lutzomyia longipalpis and macro-habitat characterization using satellite images in a leishmaniasis-endemic city in Argentina.

The spatiotemporal population dynamics of Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) were evaluated in a city in Argentina in which visceral leishmaniasis is endemic. Over 14 sampling sessions, 5244 specimens of five species of Phlebotominae (Diptera: Psychodidae) were captured, of which 2458 (46.87%) specimens were L. longipalpis. Generalized linear models were constructed to evaluate the associations between L. longipalpis abundance and explanatory variables derived from satellite images. The spatial variable 'stratum' and the temporal variable 'season' were also included in the models. Three variables were found to have significant associations: the normalized difference vegetation index; land surface temperature, and low urban coverage. The last two of these were associated with L. longipalpis abundance only during summer and winter, respectively. This variation between seasons supports the development of models that include temporal variables because models of distributions of the abundance of a species may show different critical variables according to the climatic period of the year. Abundance decreased gradually towards the downtown area, which suggests that L. longipalpis responds to a meta-population structure, in which rural-periurban source populations that persist over time may colonize adjacent areas. This information allows for a spatiotemporal stratification of risk, which provides public health authorities with a valuable tool to help optimize prevention measures against visceral leishmaniasis.

Co-occurrence and seasonal and environmental distributions of the sandflies Lutzomyia longipalpis and Nyssomyia whitmani in the city of Puerto Iguazú, northeastern Argentina.

The aim of this work was to study the distribution of Phlebotominae (Diptera: Psycodidade) abundance in time and space in an area in northeastern Argentina with vector transmission of visceral and tegumentary leishmaniasis. For this, 51 households were selected using a 'worst scenario' criterion where one light trap was set during two consecutive nights in peridomiciles in the transitions between the four seasons, and the environment was surveyed simultaneously. The relationships of phlebotomine assemblage structure and the most abundant species with seasonality and environmental variables were evaluated using a canonical correspondence analysis and generalized linear mixed models, respectively. A total of 5110 individuals were captured. Lutzomyia longipalpis (Lutz & Neiva, 1912) and Nyssomyia whitmani (Antunes & Coutinho, 1939) were the most abundant species captured in all samplings (98.3% of the total capture). The period of highest abundance of Lu. longipalpis was early autumn, and it was distributed in the most urbanized areas. Nyssomyia whitmani occupied mainly the less urbanized areas, showing peaks of abundance in early spring and summer. Other species were captured in low numbers and showed seasonal-spatial variations similar to those of Ny. whitmani. We confirmed Leishmania spp. vector persistence throughout the year in spatial patches of high abundance even during the less favorable season.

Leishmania infantum DNA detected in phlebotomine species from Puerto Iguazú City, Misiones province, Argentina.

In Puerto Iguazú City, Argentina, human and canine Visceral Leishmaniasis cases have been recorded since the year 2010, with Leishmania infantum as the etiological agent and Lutzomyia longipalpis as its main vector. In the present study, polymerase chain reaction and sequencing were used to detect L. infantum DNA in 3.9% of the female sandflies captured in Puerto Iguazú City. This is the first report of L. infantum DNA detection in Micropygomyia quinquefer, and the second one in Lu. longipalpis and Nyssomyia whitmani for Argentina. Although the detection of Leishmania DNA itself is not enough to determine a Phlebotomine species as a vector, these results are significant in setting the direction of further investigations of vectorial competence and capacity, necessary to define the roles of different sandflies species as specific or permissive vectors in the transmission VL cycle.

Evaluation of efficacy of impregnated curtains in experimental hen houses as a phlebotomine control tool in northeast Argentina.

The aim of this study was to evaluate the effectiveness of insecticide-impregnated curtains against the entry of phlebotomine (Diptera: Psychodidae) flies into experimental slatted hen houses in an area endemic for American cutaneous leishmaniasis (ACL). Three treatments in experimental dwellings containing three chickens each were applied using, respectively, an impregnated curtain (IC), a non-impregnated curtain (NIC) and no curtain (NC). A control site without chickens (WC) was included. The study used permethrin at 0.05 g/m2 . During each month for 1 year, each experimental hen house randomly received all treatments. Phlebotomine sandflies were captured using REDILA BL traps placed inside the hen house. Significant differences in abundances of phlebotomine flies/trap/night were observed between treatments (χ2 = 17853.58, d.f. = 3, P < 0.0001): 59.7% of phlebotomines were captured in the NC treatment, 26.3% in the NIC treatment, 8.0% in the IC treatment and 6.1% in the WC condition. Nyssomyia whitmani (Antunes & Coutinho) was the most abundant species in all collections (89.9%). These results showed a lower abundance of phlebotomines in the experimental hen house in the IC condition than in the hen house in the NC condition (P < 0.05) and that the presence of NIC represents an effective physical barrier against phlebotomines (P < 0.05). Therefore, the use of curtains may be an alternative eco-friendly method for the prevention of indoor ACL transmission in slatted dwellings, which represent a common house type in northeast Argentina.

Percutaneous dilatational tracheostomy using a tracheoscopic ventilation tube in an experimental ex vivo animal model.

The ETView(®) tube is a standard endotracheal tube with an embedded miniature video camera that permits real-time video imaging of the tracheal lumen. We evaluated its use when performing percutaneous dilatational tracheostomy (PDT) in an ex vivo animal model. The model consisted of a pig larynyx and trachea. The ETView tube was used as an alternative to bronchoscopy, to see all manoeuvres of PDT in real time. At the end of the PDT, operative time and any complications such as trauma to the cricoid cartilage, tracheal wall or tube cuff were assessed. Nine PDT procedures were performed by two experienced operators. The mean operative time was 7.1 ± 0.9 minutes. No complications were observed. Our study supports the use of the ETView tube as a suitable alternative to bronchoscopy when performing PDT, although clinical studies are required to confirm our experimental results. .

Análise microbiológica de vegetais e hortaliças minimamente processados / Microbiological analysis of vegetables and hortaliças most minimum prosecuted

Foram analisadas 10 amostras de vegetais e hortaliças minimamente processadas, comercializadas em hipermercados da cidade de Londrina-PR. As análises microbiológicas realizadas foram a determinação de coliformes totais e fecais, através da técnica do Numero Mais Provável (NMP) e presença de Escherichia coli através de análises bioquímicas de identificação. Os resultados obtidos para bactérias do grupo dos coliformes mostraram que 100 por cento das amostras estavam contaminadas por estes microrganismos, sendo que 80 por cento apresentaram-se fora do padrão estabelecido para coliformes fecais. Foi constatada a presença de E. coli em 80 por cento das amostras analisadas. Proteus e Enterobacter foram detectados em 20 por cento.