ABSTRACT
This work describes the first method using biochar (BC) as carbonaceous platform for immunoassay application. BC is a highly functionalized material obtained through biomass pyrolysis under controlled conditions. Due to the highly functionalized surface, covalent binding between BC and biomolecules can be performed by EDC/NHS conjugation. The application of the modified electrode was done with Hantavirus, that are etiologic agents mainly transmitted by wild rodents. Among its pathologies Hantavirus Cardiopulmonary Syndrome (HCPS) arises at Americas, caused by Hantavirus Araucária and reaches 40% lethality. The diagnostic is based on the presence of specific hantavirus nucleoprotein (Np), under viremic condition or IgG2b antibodies (Ab), during first symptoms. The results presented a device sensitivity of 5.28⯵A dec-1 and a LOD of 0.14â¯ngâ¯mL-1 to the Np detection, ranging from 5.0â¯ngâ¯mL-1 to 1.0⯵gâ¯mL-1, the Ab detection works as qualitative type sensor above 200â¯ngâ¯mL-1. Both sensors were evaluated its selectivity and serum samples; selectivity against Gumboro disease, VP2 protein, and antibody IgG2a against Yellow fever disease (YF), respectively. So, the devices here proposed are promising tool suitable for both rodent and human hantavirus clinical surveys.
Subject(s)
Charcoal/chemistry , Orthohantavirus/isolation & purification , Antibodies, Immobilized/immunology , Antibodies, Viral/immunology , Blood/virology , Electrochemical Techniques/instrumentation , Electrochemical Techniques/methods , Electrodes , Orthohantavirus/chemistry , Humans , Immunoassay/methods , Immunoglobulin G/immunology , Limit of Detection , Reproducibility of Results , Viral Proteins/immunologyABSTRACT
Targeting regions of proteins that show a high degree of structural conservation has been proposed as a method of developing immunotherapies and vaccines that may bypass the wide genetic variability of RNA viruses. Despite several attempts, a vaccine that protects evenly against the four circulating Dengue virus (DV) serotypes remains elusive. To find critical conserved amino acids in dengue viruses, 120 complete genomes of each serotype were selected at random and used to calculate conservation scores for nucleotide and amino acid sequences. The identified peptide sequences were analysed for their structural conservation and localisation using crystallographic data. The longest, surface exposed, highly conserved peptide of Envelope protein was found to correspond to amino acid residues 250 to 270. Mutation of this peptide in DV1 was lethal, since no replication of the mutant virus was detected in human cells. Antibodies against this peptide were detected in DV naturally infected patients indicating its potential antigenicity. Hence, this study has identified a highly conserved, critical peptide in DV that is a target of antibodies in infected humans.
Subject(s)
Dengue Virus/genetics , Dengue Virus/immunology , Dengue/immunology , Peptides/immunology , Viral Envelope Proteins/genetics , Amino Acid Sequence , Antibodies, Viral/metabolism , Base Sequence , Conserved Sequence , Crystallography, X-Ray , Dengue/virology , Genome, Viral , Humans , Models, Molecular , Mutation , Peptides/chemistry , Peptides/genetics , Protein Conformation , Serogroup , Viral Envelope Proteins/chemistry , Viral Envelope Proteins/immunologyABSTRACT
The Oswaldo Cruz Foundation produces most of the yellow fever (YF) vaccine prepared world wide. As part of a broader approach to determine the genetic variability in YF l7D seeds and vaccines and its relevance to viral attenuation the 17DD virus was purifed directly from chick embryo homogenates which is the source of virus used for vaccination of millions of people in Brazil and other countries for half a century. Neutralization and hemagglutination tests showed that the purified virus is similar to the original stock. Furthermore, radioimmune precipitation of 35S-methionine-labeled viral proteins using mouse hyperimmune ascitic fluid revealed identical patterns for the purified 17DD virus and the YF l7D-204 strain except for the 17DD E protein which migrated slower on SDS-PAGE. This difference is likely to be due to N-linked glycosylation. Finally, comparison by northern blot nybridization of virion RNAs of purified 17DD with two other strains of YF virus only fenome-sized molecules for all three viruses. These observations suggest that vaccine phenotype is primarily associated with the accumulation of mutations