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1.
Appl Environ Microbiol ; 78(17): 6321-6, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22773633

ABSTRACT

Bin toxin from Bacillus sphaericus acts on Culex quinquefasciatus larvae by binding to Cqm1 midgut-bound receptors, and disruption of the cqm1 gene is the major cause of resistance. The goal of this work was to screen for a laboratory-selected resistance cqm1(REC) allele in field populations in the city of Recife, Brazil, and to describe other resistance-associated polymorphisms in the cqm1 gene. The cqm1(REC) allele was detected in the four nontreated populations surveyed at frequencies from 0.001 to 0.017, and sequence analysis from these samples revealed a novel resistant allele (cqm1(REC-D16)) displaying a 16-nucletotide (nt) deletion which is distinct from the 19-nt deletion associated with cqm1(REC). Yet a third resistant allele (cqm1(REC-D25)), displaying a 25-nt deletion, was identified in samples from a treated area exposed to B. sphaericus. A comparison of the three deletion events revealed that all are located within the same 208-nt region amplified during the screening procedure. They also introduce equivalent frameshifts in the sequence and generate the same premature stop codon, leading to putative transcripts encoding truncated proteins which are unable to locate to the midgut epithelium. The populations analyzed in this study contained a variety of alleles with mutations disrupting the function of the corresponding Bin toxin receptor. Their locations reveal a hot spot that can be exploited to assess the resistance risk through DNA screening.


Subject(s)
Bacillus/pathogenicity , Bacterial Toxins/toxicity , Culex/genetics , Insect Proteins/genetics , Mutation , Receptors, Cell Surface/genetics , Alleles , Animals , Brazil , Culex/immunology , Culex/microbiology , Polymorphism, Genetic
2.
Rev. Ciênc. Méd. Biol. (Impr.) ; 6(3): 298-305, set.-dez. 2007. graf, tab
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-530618

ABSTRACT

Este trabalho visou a detectar problemas de saúde e analisar o nível de conhecimento e proteção contra os efeitos danosos da música eletronicamente amplificada (MEA) entre funcionários de danceterias de Recife. Entre março e julho de 2006, foram visitadas dez danceterias, sendo entrevistados 71 trabalhadores expostos diretamente ao barulho intenso, como seguranças, recepcionistas, garçons e caixas. Distúrbios do sono (insônia) foram citados por 33,8 por cento dos funcionários. Os problemas de saúde mais freqüentemente citados foram estresse (23,9 por cento), zumbido (22,5 por cento), sensaçãode eco (15,5 por cento) e dor de ouvido (5,6 por cento). Cerca de 75 por cento dos funcionários não utilizam proteção auditiva durante o trabalho. Esses dados sugerem que a exposição à MEA pode alterar a saúde dos trabalhadores e destacam a necessidade de maior conscientização entre funcionários e gerentes.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Hearing , Sleep Initiation and Maintenance Disorders , Noise , Noise/adverse effects , Occupational Health , Tinnitus
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