Therapeutic or lifelong training effects on pancreatic morphological and functional parameters in an animal model of aging and obesity.

AIMS: Obesity, aging, and physical training are factors influencing pancreatic functional and morphological parameters. Aiming to clarify the impact of the interaction of these factors, we analyzed the effect of therapeutic or lifelong physical training on body adiposity and pancreatic functional and morphological parameters of aged and obese rats. METHODS: 24 male Wistar rats were (initial age = 4 months and final age = 14 months) randomly divided into three aged and obese experimental groups (n = 8/group): untrained, therapeutic trained, and lifelong trained. Body adiposity, plasmatic concentration and pancreatic immunostaining of insulin, markers of tissue inflammation, lipid peroxidation, activity and immunostaining of antioxidant enzymes, and parameters of pancreatic morphology were evaluated. RESULTS: Lifelong physical training improved the body adiposity, plasmatic insulin concentration, and macrophage immunostaining in the pancreas. The animals submitted to therapeutic and lifelong training showed an increase in the density of the pancreatic islets; lower insulin, Nuclear Factor Kappa B (NF-κB), and Transforming Growth Factor beta (TGF-ß) immunostaining in the pancreatic parenchyma, as well as lower pancreatic tissue lipid peroxidation, lower fibrosis area, increased catalase and glutathione peroxidase (GPx) activity and increased heme oxygenase-1 (HO-1) immunostaining, with the greatest effect in the lifelong training group. CONCLUSION: Lifelong training promoted greater beneficial effects on the pancreatic functional and morphological parameters of aged and obese animals compared to therapeutic exercise.

Human papillomavirus detection in cervical scrapes from women attended in the Family Health Program.

OBJECTIVES: to survey the prevalence of human papillomavirus, associated risk factors and genotype distribution in women who were referred to cervical cancer screening when attended in a Family Health Program. METHOD: we conducted a cross-sectional survey, investigating 351 women. Polymerase chain reaction for DNA amplification and restriction fragment length polymorphism analysis were used to detect and typify the papillomavirus. RESULTS: virus infection was detected in 8.8% of the samples. Among the 21 different genotypes identified in this study, 14 were high risk for cervical cancer, and the type 16 was the most prevalent type. The infection was associated with women who had non-stable sexual partners. Low risk types were associated with younger women, while the high risk group was linked to altered cytology. CONCLUSION: in this sample attended a Family Health Program, we found a low rate of papillomavirus infection. Virus frequency was associated to sexual behavior. However, the broad range of genotypes detected deserves attention regarding the vaccine coverage, which includes only HPV prevalent types.

Human papillomavirus detection in cervical scrapes from women attended in the Family Health Program / Detecção do papilomavírus humano em citologias cervicais de mulheres atendidas no Programa Saúde da Família / Detección del papilomavirus humano en citologías cervicales de mujeres atendidas en el Programa de Salud de la Familia

OBJECTIVES: to survey the prevalence of human papillomavirus, associated risk factors and genotype distribution in women who were referred to cervical cancer screening when attended in a Family Health Program. METHOD: we conducted a cross-sectional survey, investigating 351 women. Polymerase chain reaction for DNA amplification and restriction fragment length polymorphism analysis were used to detect and typify the papillomavirus. RESULTS: virus infection was detected in 8.8% of the samples. Among the 21 different genotypes identified in this study, 14 were high risk for cervical cancer, and the type 16 was the most prevalent type. The infection was associated with women who had non-stable sexual partners. Low risk types were associated with younger women, while the high risk group was linked to altered cytology. CONCLUSION: in this sample attended a Family Health Program, we found a low rate of papillomavirus infection. Virus frequency was associated to sexual behavior. However, the broad range of genotypes detected deserves attention regarding the vaccine coverage, which includes only HPV prevalent types. .

OBJETIVOS: identificar a prevalência do papilomavírus humano, os fatores de risco associados e a distribuição dos genótipos nas mulheres referenciadas para rastreamento do câncer cervical, durante o atendimento no Programa de Saúde da Família. MÉTODO: foi realizado levantamento de corte transversal com 351 mulheres. A amplificação do DNA por reação da cadeia da polimerase e a análise do polimorfismo de fragmentos de restrição foram aplicadas para detectar e tipificar o papilomavírus. RESULTADOS: a infecção pelo vírus foi detectada em 8,8% das amostras. Entre os 21 genótipos diferentes identificados neste estudo, 14 foram de alto risco para o câncer cervical, e o tipo 16 foi o mais prevalente. A infecção foi associada a mulheres com parceiros sexuais diversos. Os tipos de baixo risco foram associados com mulheres mais novas, enquanto o grupo de alto risco foi vinculado a alterações citológicas. CONCLUSÃO: nesta amostra, atendida por um Programa de Saúde da Família, encontraram-se níveis baixos de infecção pelo papilomavírus. Foi detectada, também, associação entre a frequência do vírus e o comportamento sexual. Porém, a grande variedade de genótipos detectados merece atenção relacionada à cobertura vacinal, que abrange somente tipos prevalentes de HPV. .

OBJETIVOS: identificar la prevalencia del papilomavirus humano, los factores de riesgo asociados y la distribución de los genotipos en las mujeres referenciadas para rastreo del cáncer cervical durante la atención en el Programa de Salud de la Familia. MÉTODO: fue efectuado un levantamiento trasversal con 351 mujeres. La amplificación del DNA por reacción de la cadena de la polimerasa y el análisis del polimorfismo de fragmentos de restricción fueron aplicadas para detectar y tipificar el papilomavirus. RESULTADOS: la infección por el virus fue detectada en el 8,8% de las muestras. Entre los 21 genotipos diferentes identificados en este estudio, 14 fueron de alto riesgo para el cáncer cervical, y el tipo 16 fue el más prevalente. La infección fue asociada con mujeres con parejas sexuales diversas. Los tipos de bajo riesgo fueron asociados con mujeres más nuevas, mientras el grupo de alto riesgo fue simplemente vinculado a alteraciones citológicas. CONCLUSIÓN: en esta muestra atendida por un Programa de Salud de la Familia, encontramos niveles bajos de infección por el papilomavirus. Fue encontrada una asociación entre la frecuencia del virus y el comportamiento sexual. Sin embargo, la gran variedad de genotipos detectados merece atención relacionada a la cobertura de vacunación, que abarca solamente tipos prevalentes de HPV. .